Mouse Anti-CA4 Recombinant Antibody (CBFYC-0696) (CBMAB-C0750-FY)

Name: Mouse Anti-CA4 Recombinant Antibody (CBFYC-0696)
SKU: CBMAB-C0750-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYC-0696

Application

E, WB, IF, IP

Immunogen

Amino acids 1-50 of CA3 of mouse origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a, κ

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

0.09% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Carbonic Anhydrase 4

Introduction

This gene encodes a glycosylphosphatidyl-inositol-anchored membrane isozyme expressed on the luminal surfaces of pulmonary (and certain other) capillaries and proximal renal tubules.

Entrez Gene ID

762

UniProt ID

P22748

Alternative Names

CAIV; Car4; RP17

Function

Reversible hydration of carbon dioxide. May stimulate the sodium/bicarbonate transporter activity of SLC4A4 that acts in pH homeostasis. It is essential for acid overload removal from the retina and retina epithelium, and acid release in the choriocapillaris in the choroid.

Biological Process

Bicarbonate transport Source: DFLAT
One-carbon metabolic process Source: GO_Central

Cellular Location

Cell membrane

Involvement in disease

Retinitis pigmentosa 17 (RP17): The disease is caused by variants affecting the gene represented in this entry. Defective acid overload removal from retina and retinal epithelium, due to mutant CA4, is responsible for photoreceptor degeneration, indicating that impaired pH homeostasis is the most likely cause underlying the RP17 phenotype. A retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.

Xu, Y., Xu, W. H., Shi, S. N., Yang, X. L., Ren, Y. R., Zhuang, X. Y., ... & Zhang, X. F. (2020). Carbonic Anhydrase 4 serves as a Clinicopathological Biomarker for Outcomes and Immune Infiltration in Renal Cell Carcinoma, Lower Grade Glioma, Lung Adenocarcinoma and Uveal Melanoma. Journal of Cancer, 11(20), 6101.

Rudenko, N. N., Fedorchuk, T. P., Terentyev, V. V., Dymova, O. V., Naydov, I. A., Golovko, T. K., ... & Ivanov, B. N. (2020). The role of carbonic anhydrase α-CA4 in the adaptive reactions of photosynthetic apparatus: the study with α-CA4 knockout plants. Protoplasma, 257(2), 489-499.

Wang, B., Jiang, H., Wan, X., Wang, Y., Zheng, X., Li, P., ... & Song, H. (2020). Carbonic anhydrase IV inhibits cell proliferation in gastric cancer by regulating the cell cycle. Oncology Letters, 20(4), 1-1.

Zhou, Y., Vroegop-Vos, I. A., Van Dijken, A. J., Van der Does, D., Zipfel, C., Pieterse, C. M., & Van Wees, S. C. (2020). Carbonic anhydrases CA1 and CA4 function in atmospheric CO 2-modulated disease resistance. Planta, 251(4), 1-14.

Chew, S. F., Koh, C. Z., Hiong, K. C., Choo, C. Y., Wong, W. P., Neo, M. L., & Ip, Y. K. (2019). Light-enhanced expression of Carbonic Anhydrase 4-like supports shell formation in the fluted giant clam Tridacna squamosa. Gene, 683, 101-112.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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