Mouse Anti-CA7 Recombinant Antibody (CBLC044-LY) (CBMAB-C9591-LY)

Name: Mouse Anti-CA7 Recombinant Antibody (CBLC044-LY)
SKU: CBMAB-C9591-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBLC044-LY

Application

E, WB, IF, IP

Immunogen

Amino acids 1-90 mapping at the N-terminus of CA VII of human origin.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

0.09% sodium azide

Concentration

0.2 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

carbonic anhydrase VII

Introduction

Carbonic anhydrases are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. The cytosolic protein encoded by this gene is predominantly expressed in the brain and contributes to bicarbonate driven GABAergic neuron excitation. Alternative splicing in the coding region results in multiple transcript variants encoding different isoforms.

Entrez Gene ID

766

UniProt ID

P43166

Alternative Names

CA-VII, CAVII

Function

Reversible hydration of carbon dioxide.

Biological Process

Bicarbonate transport Source: Reactome
One-carbon metabolic process Source: GO_Central
Positive regulation of cellular pH reduction Source: Ensembl
Positive regulation of synaptic transmission, GABAergic Source: Ensembl
Regulation of chloride transport Source: Ensembl
Regulation of intracellular pH Source: GO_Central

Cellular Location

Cytoplasm

Sumi, K. R., Kim, S. C., Howlader, J., Sharker, M. R., Choi, K. S., Choi, S. K., ... & Kho, K. H. (2019). Molecular identification and expression analysis of carbonic anhydrase VII in Pufferfish (Takifugu rubripes). Ocean Science Journal, 54(3), 363-374.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Mouse Anti-CA7 Recombinant Antibody (H4) (CAT#: CBMAB-C5745-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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