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Mouse Anti-CALCOCO1 Recombinant Antibody (CBXC-1732) (CBMAB-C5836-CQ)


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Published Data
CBMAB-C5836-CQ-1
Mouse Anti-CALCOCO1 Recombinant Antibody (CBXC-1732) (CBMAB-C5836-CQ) in IF
Representative confocal images of HeLa CALCOCO1 KO cells transiently co-transfected with mCherry-CALCOCO1, Myc-VAPA, and EGFP-LAMP1, and then treated as indicated before immunostaining with anti-Myc antibody. Arrows indicate co-localization. ...View More
Nthiga, T. M., Kumar Shrestha, B., Sjøttem, E., Bruun, J. A., Bowitz Larsen, K., Bhujabal, Z., ... & Johansen, T. (2020). CALCOCO 1 acts with VAMP-associated proteins to mediate ER-phagy. The EMBO Journal, 39(15), e103649.
CBMAB-C5836-CQ-2
Mouse Anti-CALCOCO1 Recombinant Antibody (CBXC-1732) (CBMAB-C5836-CQ) in IF
WT or CALCOCO1 KO HeLa cells were transiently transfected with mCherry-EGFP-VAPA or mCherry-EGFP-FAM134B as indicated and 24 h after transfection, the cells were treated or not with HBSS as indicated. ...View More
Nthiga, T. M., Kumar Shrestha, B., Sjøttem, E., Bruun, J. A., Bowitz Larsen, K., Bhujabal, Z., ... & Johansen, T. (2020). CALCOCO 1 acts with VAMP-associated proteins to mediate ER-phagy. The EMBO Journal, 39(15), e103649.
CBMAB-C5836-CQ-3
Mouse Anti-CALCOCO1 Recombinant Antibody (CBXC-1732) (CBMAB-C5836-CQ) in Immunoblot
Immunoblot analysis of HeLa CALCOCO1 KO cell lines reconstituted with EGFP-CALCOCO1 Δ145-513. Expression of EGFP-CALCOCO1 Δ145-513 was induced or not with tetracycline, and the cells were treated with MG132, HBSS, or Baf A1 as indicated. ...View More
Nthiga, T. M., Kumar Shrestha, B., Sjøttem, E., Bruun, J. A., Bowitz Larsen, K., Bhujabal, Z., ... & Johansen, T. (2020). CALCOCO 1 acts with VAMP-associated proteins to mediate ER-phagy. The EMBO Journal, 39(15), e103649.

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBXC-1732
Antibody Isotype
IgG1,
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 118-343 mapping within an internal region of CALCOCO1 of human origin.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1,
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA1:100-1:1,000
IF(ICC)1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Calcium Binding And Coiled-Coil Domain 1
Introduction
CALCOCO1 (Calcium Binding And Coiled-Coil Domain 1) is a Protein Coding gene. Gene Ontology (GO) annotations related to this gene include sequence-specific DNA binding and transcription coactivator activity. An important paralog of this gene is TAX1BP1.
Entrez Gene ID
57658
UniProt ID
Q9P1Z2
Alternative Names
Calcium Binding And Coiled-Coil Domain 1; Coiled-Coil Leucine Zipper Coactivator 1; Inorganic Pyrophosphatase Activator; Coiled-Coil Coactivator Protein; Sarcoma Antigen NY-SAR-3; Calphoglin;
Function
Functions as a coactivator for aryl hydrocarbon and nuclear receptors (NR). Recruited to promoters through its contact with the N-terminal basic helix-loop-helix-Per-Arnt-Sim (PAS) domain of transcription factors or coactivators, such as NCOA2. During ER-activation acts synergistically in combination with other NCOA2-binding proteins, such as EP300, CREBBP and CARM1. Involved in the transcriptional activation of target genes in the Wnt/CTNNB1 pathway. Functions as a secondary coactivator in LEF1-mediated transcriptional activation via its interaction with CTNNB1. Coactivator function for nuclear receptors and LEF1/CTNNB1 involves differential utilization of two different activation regions (By similarity).
In association with CCAR1 enhances GATA1- and MED1-mediated transcriptional activation from the gamma-globin promoter during erythroid differentiation of K562 erythroleukemia cells (PubMed:24245781).
Seems to enhance inorganic pyrophosphatase thus activating phosphogluomutase (PMG). Probably functions as component of the calphoglin complex, which is involved in linking cellular metabolism (phosphate and glucose metabolism) with other core functions including protein synthesis and degradation, calcium signaling and cell growth.
Biological Process
Intracellular steroid hormone receptor signaling pathway Source: HGNC-UCL
Positive regulation of gene expression Source: AgBase
Positive regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: HGNC-UCL
Signal transduction Source: HGNC-UCL
Wnt signaling pathway Source: UniProtKB-KW
Cellular Location
Cytoplasm; Nucleus. Shuttles between nucleus and cytoplasm.
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For research use only. Not intended for any clinical use.

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