Mouse Anti-CALCOCO1 Recombinant Antibody (CBXC-1732) (CBMAB-C5836-CQ)

Name: Mouse Anti-CALCOCO1 Recombinant Antibody (CBXC-1732)
SKU: CBMAB-C5836-CQ
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CALCOCO1 Recombinant Antibody (CBXC-1732) (CBMAB-C5836-CQ) in IF

Representative confocal images of HeLa CALCOCO1 KO cells transiently co-transfected with mCherry-CALCOCO1, Myc-VAPA, and EGFP-LAMP1, and then treated as indicated before immunostaining with anti-Myc antibody. Arrows indicate co-localization. ...View More

Nthiga, T. M., Kumar Shrestha, B., Sjøttem, E., Bruun, J. A., Bowitz Larsen, K., Bhujabal, Z., ... & Johansen, T. (2020). CALCOCO 1 acts with VAMP-associated proteins to mediate ER-phagy. The EMBO Journal, 39(15), e103649.

Mouse Anti-CALCOCO1 Recombinant Antibody (CBXC-1732) (CBMAB-C5836-CQ) in IF

WT or CALCOCO1 KO HeLa cells were transiently transfected with mCherry-EGFP-VAPA or mCherry-EGFP-FAM134B as indicated and 24 h after transfection, the cells were treated or not with HBSS as indicated. ...View More

Mouse Anti-CALCOCO1 Recombinant Antibody (CBXC-1732) (CBMAB-C5836-CQ) in Immunoblot

Immunoblot analysis of HeLa CALCOCO1 KO cell lines reconstituted with EGFP-CALCOCO1 Δ145-513. Expression of EGFP-CALCOCO1 Δ145-513 was induced or not with tetracycline, and the cells were treated with MG132, HBSS, or Baf A1 as indicated. ...View More

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBXC-1732

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 118-343 mapping within an internal region of CALCOCO1 of human origin.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1,

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:100-1:1,000
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Calcium Binding And Coiled-Coil Domain 1

Introduction

CALCOCO1 (Calcium Binding And Coiled-Coil Domain 1) is a Protein Coding gene. Gene Ontology (GO) annotations related to this gene include sequence-specific DNA binding and transcription coactivator activity. An important paralog of this gene is TAX1BP1.

Entrez Gene ID

57658

UniProt ID

Q9P1Z2

Alternative Names

Calcium Binding And Coiled-Coil Domain 1; Coiled-Coil Leucine Zipper Coactivator 1; Inorganic Pyrophosphatase Activator; Coiled-Coil Coactivator Protein; Sarcoma Antigen NY-SAR-3; Calphoglin;

Function

Functions as a coactivator for aryl hydrocarbon and nuclear receptors (NR). Recruited to promoters through its contact with the N-terminal basic helix-loop-helix-Per-Arnt-Sim (PAS) domain of transcription factors or coactivators, such as NCOA2. During ER-activation acts synergistically in combination with other NCOA2-binding proteins, such as EP300, CREBBP and CARM1. Involved in the transcriptional activation of target genes in the Wnt/CTNNB1 pathway. Functions as a secondary coactivator in LEF1-mediated transcriptional activation via its interaction with CTNNB1. Coactivator function for nuclear receptors and LEF1/CTNNB1 involves differential utilization of two different activation regions (By similarity).
In association with CCAR1 enhances GATA1- and MED1-mediated transcriptional activation from the gamma-globin promoter during erythroid differentiation of K562 erythroleukemia cells (PubMed:24245781).
Seems to enhance inorganic pyrophosphatase thus activating phosphogluomutase (PMG). Probably functions as component of the calphoglin complex, which is involved in linking cellular metabolism (phosphate and glucose metabolism) with other core functions including protein synthesis and degradation, calcium signaling and cell growth.

Biological Process

Intracellular steroid hormone receptor signaling pathway Source: HGNC-UCL
Positive regulation of gene expression Source: AgBase
Positive regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: HGNC-UCL
Signal transduction Source: HGNC-UCL
Wnt signaling pathway Source: UniProtKB-KW

Cellular Location

Cytoplasm; Nucleus. Shuttles between nucleus and cytoplasm.

Nthiga, T. M., Shrestha, B. K., Bruun, J. A., Larsen, K. B., Lamark, T., & Johansen, T. (2021). Regulation of Golgi turnover by CALCOCO1-mediated selective autophagy. Journal of Cell Biology, 220(6), e202006128.

Stefely, J. A., Zhang, Y., Freiberger, E. C., Kwiecien, N. W., Thomas, H. E., Davis, A. M., ... & Mercer, C. A. (2020). Mass spectrometry proteomics reveals a function for mammalian CALCOCO1 in MTOR-regulated selective autophagy. Autophagy, 16(12), 2219-2237.

Nthiga, T. M., Shrestha, B. K., Lamark, T., & Johansen, T. (2020). CALCOCO1 is a soluble reticulophagy receptor. Autophagy, 16(9), 1729-1731.

Yu, G., Xiong, D., Liu, Z., Li, Y., Chen, K., & Tang, H. (2019). Long noncoding RNA LINC00052 inhibits colorectal cancer metastasis by sponging microRNA‐574‐5p to modulate CALCOCO1 expression. Journal of cellular biochemistry, 120(10), 17258-17272.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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