Mouse Anti-CALY Recombinant Antibody (G-8) (CBMAB-1292-CN)

Name: Mouse Anti-CALY Recombinant Antibody (G-8)
SKU: CBMAB-1292-CN
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

G-8

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 4-178 deletion 134-151 (deletion 134-151) mapping within an internal region of Calcyon of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:100-1:1,000
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Calcyon Neuron Specific Vesicular Protein

Introduction

The protein encoded by this gene is a type II single transmembrane protein. It is required for maximal stimulated calcium release after stimulation of purinergic or muscarinic but not beta-adrenergic receptors. The encoded protein interacts with D1 dopamine receptor and may interact with other DA receptor subtypes and/or GPCRs.

Entrez Gene ID

50632

UniProt ID

Q9NYX4

Alternative Names

NSG3; DRD1IP

Function

Interacts with clathrin light chain A and stimulates clathrin self-assembly and clathrin-mediated endocytosis.

Biological Process

Anterograde axonal transport Source: Ensembl
Clathrin coat assembly Source: UniProtKB
Dopamine receptor signaling pathway Source: InterPro
Endosomal transport Source: GO_Central
Positive regulation of clathrin coat assembly Source: Ensembl
Positive regulation of endocytosis Source: UniProtKB
Positive regulation of retrograde axon cargo transport Source: Ensembl
Postsynaptic neurotransmitter receptor internalization Source: Ensembl

Cellular Location

Cell membrane; Cytoplasmic vesicle membrane

Topology

Extracellular: 1-87 aa
Helical: 88-108 aa
Cytoplasmic: 109-217 aa

PTM

Glycosylated.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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