Mouse Anti-CAPN7 Recombinant Antibody (CBFYC-0800) (CBMAB-C0855-FY)

Name: Mouse Anti-CAPN7 Recombinant Antibody (CBFYC-0800)
SKU: CBMAB-C0855-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYC-0800

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 1-300 mapping at the N-terminus of Calpain 7 of human origin.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:100-1:1,000
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Calpain 7

Introduction

CAPN7 (Calpain 7) is a Protein Coding gene. Among its related pathways are Integrin Pathway and Apoptosis Pathway. Gene Ontology (GO) annotations related to this gene include endopeptidase activity and MIT domain binding. An important paralog of this gene is CAPN15.

Entrez Gene ID

23473

UniProt ID

Q9Y6W3

Alternative Names

Calpain 7; Homolog Of Aspergillus Nidulans PALB; Calpain Like Protease; PalB Homolog; PALBH; EC 3.4.22.-; Calpain-7; EC 3.4.22; CALPAIN7

Function

Calcium-regulated non-lysosomal thiol-protease.

Biological Process

Positive regulation of epithelial cell migration Source: UniProtKB
Self proteolysis Source: UniProtKB

Cellular Location

Nucleus

Maki, M. (2020). Structures and functions of penta-EF-hand calcium-binding proteins and their interacting partners: Enigmatic relationships between ALG-2 and calpain-7. Bioscience, biotechnology, and biochemistry, 84(4), 651-660.

Yan, Q., Jiang, R., Huang, C., Yan, G., & Sun, H. (2017). CAPN7 negatively regulates embryo adhesion via cleaving hoxa10. Fertility and Sterility, 108(3), e213.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Mouse Anti-CAPN7 Recombinant Antibody (CBYY-C0938) (CAT#: CBMAB-C2375-YY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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