Mouse Anti-CARD10 Recombinant Antibody (EG478) (CBMAB-EN542-LY)

Name: Mouse Anti-CARD10 Recombinant Antibody (EG478)
SKU: CBMAB-EN542-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

EG478

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 857-877 near the C-terminus of CARD 10 of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat, Pig

Antibody Isotype

IgG3, κ

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:100-1:1,000
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Caspase Recruitment Domain Family Member 10

Introduction

The caspase recruitment domain (CARD) is a protein module that consists of 6 or 7 antiparallel alpha helices. It participates in apoptosis signaling through highly specific protein-protein homophilic interactions. Like several other CARD proteins, CARD10 belongs to the membrane-associated guanylate kinase (MAGUK) family and activates NF-kappa-B (NFKB; see MIM 164011) through BCL10 (MIM 603517) (Wang et al., 2001 [PubMed 11259443]).[supplied by OMIM, Mar 2008]

Entrez Gene ID

Human	29775
Mouse	105844

UniProt ID

Human	Q9BWT7
Mouse	P58660

Alternative Names

Caspase Recruitment Domain Family Member 10; CARD-Containing MAGUK Protein 3; Carma 3; CARMA3; Caspase Recruitment Domain-Containing Protein 10; Caspase Recruitment Domain Family, Member 10; Bcl10 Binding Protein And Activator Of NFKB; CARD-Containing MAGUK 3 Protein; BIMP1;

Function

Activates NF-kappa-B via BCL10 and IKK.

Biological Process

Activation of NF-kappaB-inducing kinase activity Source: UniProtKB
Negative regulation of cell migration involved in sprouting angiogenesis Source: BHF-UCL
Positive regulation of protein localization to nucleus Source: BHF-UCL
Protein-containing complex assembly Source: UniProtKB
Regulation of apoptotic process Source: InterPro

Cellular Location

Cytoplasm

Israël, L., Glück, A., Berger, M., Coral, M., Ceci, M., Unterreiner, A., ... & Bornancin, F. (2021). CARD10 cleavage by MALT1 restricts lung carcinoma growth in vivo. Oncogenesis, 10(4), 1-11.

Yang, D. H., Guo, T., Yuan, Z. Z., Lei, C., Ding, S. Z., Yang, Y. F., ... & Luo, H. (2020). Mutant CARD10 in a family with progressive immunodeficiency and autoimmunity. Cellular & molecular immunology, 17(7), 782-784.

Zhang, C., Wang, H., & Yang, B. (2020). miR-146a regulates inflammation and development in patients with abdominal aortic aneurysms by targeting CARD10. International angiology: a journal of the International Union of Angiology.

Peng, L., He, K., Cao, Z., Bi, L., Yu, D., Wang, Q., & Wang, J. (2020). CARD10 promotes the progression of renal cell carcinoma by regulating the NF‑κB signaling pathway. Molecular medicine reports, 21(1), 329-337.

Motawi, T. K., El-Maraghy, S. A., Sharaf, S. A., & Said, S. E. (2019). Association of CARD10 rs6000782 and TNF rs1799724 variants with paediatric-onset autoimmune hepatitis. Journal of advanced research, 15, 103-110.

Liu, X., Zhang, X., Bi, J., Li, Z., Zhang, Z., & Kong, C. (2019). Caspase recruitment domain family member 10 regulates carbamoyl phosphate synthase 1 and promotes cancer growth in bladder cancer cells. Journal of cellular and molecular medicine, 23(12), 8128-8138.

Israël, L., Bardet, M., Huppertz, A., Mercado, N., Ginster, S., Unterreiner, A., ... & Bornancin, F. (2018). A CARD10-dependent tonic signalosome activates MALT1 paracaspase and regulates IL-17/TNF-α-driven keratinocyte inflammation. The Journal of investigative dermatology, 138(9), 2075-2079.

Yamamoto-Furusho, J. K., Fonseca-Camarillo, G., Furuzawa-Carballeda, J., Sarmiento-Aguilar, A., Barreto-Zuñiga, R., Martínez-Benitez, B., & Lara-Velazquez, M. A. (2018). Caspase recruitment domain (CARD) family (CARD9, CARD10, CARD11, CARD14 and CARD15) are increased during active inflammation in patients with inflammatory bowel disease. Journal of Inflammation, 15(1), 1-12.

Zou, Y., Zhou, J. Y., Wang, F., Zhang, Z. Y., Liu, F. Y., Luo, Y., ... & Huang, O. P. (2018). Analysis of CARD10 and CARD11 somatic mutations in patients with ovarian endometriosis. Oncology letters, 16(1), 491-496.

Shyamsunder, P., Sankar, H., Mayakonda, A., Han, L., Nordin, H. B. M., Woon, T. W., ... & Koeffler, H. P. (2018). CARD10, a CEBPE target involved in granulocytic differentiation. haematologica, 103(8), 1269.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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