Mouse Anti-CARF Recombinant Antibody (CBXC-1533) (CBMAB-C5524-CQ)

Name: Mouse Anti-CARF Recombinant Antibody (CBXC-1533)
SKU: CBMAB-C5524-CQ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBXC-1533

Application

ELISA, WB

Immunogen

CARF (1aa-579aa) Full length recombinant protein with GST tag.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Calcium Responsive Transcription Factor

Introduction

CARF (Calcium Responsive Transcription Factor) is a Protein Coding gene. Gene Ontology (GO) annotations related to this gene include DNA binding transcription factor activity and transcriptional activator activity, RNA polymerase II proximal promoter sequence-specific DNA binding.

Entrez Gene ID

79800

UniProt ID

Q8N187

Alternative Names

Calcium Responsive Transcription Factor; Calcium-Response Factor; Amyotrophic Lateral Sclerosis 2 Chromosomal Region Candidate Gene 8 Protein; Amyotrophic Lateral Sclerosis 2 (Juvenile) Chromosome Region, Candidate 8; Testis Development Protein NYD-SP24; ALS2CR8; Calcium-Responsive Transcription Factor; NYD-SP24; CaRF;

Function

Acts as a transcriptional activator that mediates the calcium- and neuron-selective induction of BDNF exon III transcription. Binds to the consensus calcium-response element CaRE1 5'-CTATTTCGAG-3' sequence.

Biological Process

Cellular response to calcium ion Source: UniProtKB
Cellular response to potassium ion Source: UniProtKB
Positive regulation of transcription from RNA polymerase II promoter in response to calcium ion Source: UniProtKB
Regulation of DNA-binding transcription factor activity Source: Ensembl
Regulation of transcription by RNA polymerase II Source: GO_Central

Cellular Location

Nucleus

Dong, W., Cao, Z., Pang, Y., Feng, T., & Tian, H. (2019). Carf, as an oncogene, promotes colorectal cancer stemness by activating erbb signaling pathway. OncoTargets and therapy, 12, 9041.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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