Mouse Anti-CASQ2 Recombinant Antibody (1C6) (CBMAB-C0492-LY)

Name: Mouse Anti-CASQ2 Recombinant Antibody (1C6)
SKU: CBMAB-C0492-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

1C6

Application

ELISA, IHC, WB

Immunogen

CASQ2 (20aa-399aa) full-length recombinant protein with GST tag.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IHC	3 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Calsequestrin 2

Introduction

The protein encoded by this gene specifies the cardiac muscle family member of the calsequestrin family. Calsequestrin is localized to the sarcoplasmic reticulum in cardiac and slow skeletal muscle cells. The protein is a calcium binding protein that stores calcium for muscle function. Mutations in this gene cause stress-induced polymorphic ventricular tachycardia, also referred to as catecholaminergic polymorphic ventricular tachycardia 2 (CPVT2), a disease characterized by bidirectional ventricular tachycardia that may lead to cardiac arrest. [provided by RefSeq, Jul 2008]

Entrez Gene ID

845

UniProt ID

O14958

Alternative Names

Calsequestrin 2; Calsequestrin, Cardiac Muscle Isoform; Calsequestrin 2 (Cardiac Muscle); Calsequestrin 2, Fast-Twitch, Cardiac Muscle; Calsequestrin-2; PDIB2;

Function

Calsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle. Calcium ions are bound by clusters of acidic residues at the protein surface, especially at the interface between subunits. Can bind around 60 Ca2+ ions. Regulates the release of lumenal Ca2+ via the calcium release channel RYR2; this plays an important role in triggering muscle contraction. Plays a role in excitation-contraction coupling in the heart and in regulating the rate of heart beats.

Biological Process

Cardiac muscle contraction Source: BHF-UCL
Cellular response to caffeine Source: BHF-UCL
Detection of calcium ion Source: BHF-UCL
Ion transmembrane transport Source: Reactome
Negative regulation of potassium ion transmembrane transporter activity Source: BHF-UCL
Negative regulation of potassium ion transport Source: BHF-UCL
Negative regulation of ryanodine-sensitive calcium-release channel activity Source: BHF-UCL
Protein polymerization Source: UniProtKB
Purkinje myocyte to ventricular cardiac muscle cell signaling Source: BHF-UCL
Regulation of cardiac conduction Source: Reactome
Regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion Source: BHF-UCL
Regulation of cell communication by electrical coupling Source: BHF-UCL
Regulation of heart rate Source: UniProtKB
Regulation of membrane repolarization Source: BHF-UCL
Regulation of release of sequestered calcium ion into cytosol Source: GO_Central
Regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum Source: BHF-UCL
Sequestering of calcium ion Source: BHF-UCL
Striated muscle contraction Source: ProtInc

Cellular Location

Sarcoplasmic reticulum lumen. This isoform of calsequestrin occurs in the sarcoplasmic reticulum's terminal cisternae luminal spaces of cardiac and slow skeletal muscle cells.

Involvement in disease

Ventricular tachycardia, catecholaminergic polymorphic, 2 (CPVT2): An arrhythmogenic disorder characterized by stress-induced, bidirectional ventricular tachycardia that may degenerate into cardiac arrest and cause sudden death. Patients present with recurrent syncope, seizures, or sudden death after physical activity or emotional stress. CPVT2 inheritance is autosomal recessive.

PTM

Phosphorylation in the C-terminus, probably by CK2, moderately increases calcium buffering capacity.
N-glycosylated.

Kim, J. H., Lee, E. S., Yun, J., Ryu, H. S., Kim, H. K., Ju, Y. W., ... & Moon, H. G. (2021). Calsequestrin 2 overexpression in breast cancer increases tumorigenesis and metastasis by modulating the tumor microenvironment. Molecular Oncology.

Valle, G., Arad, M., & Volpe, P. (2020). Molecular adaptation to calsequestrin 2 (CASQ2) point mutations leading to catecholaminergic polymorphic ventricular tachycardia (CPVT): comparative analysis of R33Q and D307H mutants. Journal of muscle research and cell motility, 41(2), 251-258.

Ross, S., Holliday, M., Lim, S., & Semsarian, C. (2019). Characterization of the first induced pluripotent stem cell line generated from a patient with autosomal dominant catecholaminergic polymorphic ventricular tachycardia due to a heterozygous mutation in cardiac calsequestrin-2. Stem cell research, 37, 101450.

Kim, J. H., sil Hong, B., Heo, W., Han, J. M., Han, W., Noh, D. Y., & Moon, H. G. (2018). Calsequestrin 2 regulates proliferation, migration, and invasion in triple-negative breast cancer cells.

Rebrova, T. Y., Muslimova, E. F., Kondratieva, D. S., Budnikova, O. V., Ahmedov, S. D., Afanasiev, S. A., & Popov, S. V. (2018). The Role of Ca 2+-ATPase 2a (ATP2A2), Ryanodine Receptors (RYR2), and Calsequestrin (CASQ2) Gene Polymorphisms in the Development of Heart Failure. Russian Journal of Genetics, 54(6), 604-608.

Josephs, K., Patel, K., Janson, C. M., Montagna, C., & McDonald, T. V. (2017). Compound heterozygous CASQ 2 mutations and long‐term course of catecholaminergic polymorphic ventricular tachycardia. Molecular genetics & genomic medicine, 5(6), 788-794.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

CASQ2 Matched Antibody Pair (184) (CAT#: APMAB-184LY)

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Mouse Anti-CASQ2 Recombinant Antibody (4E10) (CAT#: CBMAB-C1863-LY)

CASQ2 Matched Antibody Pair (184) (CAT#: APMAB-184LY)

Isotype control

For research use only. Not intended for any clinical use.

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