Mouse Anti-CCAR2 Recombinant Antibody (CBYCD-125) (CBMAB-D0036-YC)

cell cycle and apoptosis regulator 2

CCAR2 (Cell Cycle And Apoptosis Regulator 2) is a protein coding gene. Diseases associated with CCAR2 include Estrogen-Receptor Negative Breast Cancer. Among its related pathways are Circadian rythm related genes and Cellular response to heat stress.

Cell Cycle And Apoptosis Regulator 2; KIAA1967; Cell Division Cycle And Apoptosis Regulator Protein 2; P30 DBC; DBC-1; NET35; DBC1; Cell Cycle And Apoptosis Regulator Protein 2;

Core component of the DBIRD complex, a multiprotein complex that acts at the interface between core mRNP particles and RNA polymerase II (RNAPII) and integrates transcript elongation with the regulation of alternative splicing: the DBIRD complex affects local transcript elongation rates and alternative splicing of a large set of exons embedded in (A + T)-rich DNA regions (PubMed:22446626).
Inhibits SIRT1 deacetylase activity leading to increasing levels of p53/TP53 acetylation and p53-mediated apoptosis (PubMed:18235501, PubMed:18235502, PubMed:23352644).
Inhibits SUV39H1 methyltransferase activity (PubMed:19218236).
Mediates ligand-dependent transcriptional activation by nuclear hormone receptors (PubMed:19131338).
Plays a critical role in maintaining genomic stability and cellular integrity following UV-induced genotoxic stress (PubMed:23398316).
Regulates the circadian expression of the core clock components NR1D1 and ARNTL/BMAL1 (PubMed:23398316).
Enhances the transcriptional repressor activity of NR1D1 through stabilization of NR1D1 protein levels by preventing its ubiquitination and subsequent degradation (PubMed:23398316).
Represses the ligand-dependent transcriptional activation function of ESR2 (PubMed:20074560).
Acts as a regulator of PCK1 expression and gluconeogenesis by a mechanism that involves, at least in part, both NR1D1 and SIRT1 (PubMed:24415752).
Negatively regulates the deacetylase activity of HDAC3 and can alter its subcellular localization (PubMed:21030595).
Positively regulates the beta-catenin pathway (canonical Wnt signaling pathway) and is required for MCC-mediated repression of the beta-catenin pathway (PubMed:24824780).
Represses ligand-dependent transcriptional activation function of NR1H2 and NR1H3 and inhibits the interaction of SIRT1 with NR1H3 (PubMed:25661920).
Plays an important role in tumor suppression through p53/TP53 regulation; stabilizes p53/TP53 by affecting its interaction with ubiquitin ligase MDM2 (PubMed:25732823).
Represses the transcriptional activator activity of BRCA1 (PubMed:20160719).
Inhibits SIRT1 in a CHEK2 and PSEM3-dependent manner and inhibits the activity of CHEK2 in vitro (PubMed:25361978).

Cell cycle Source: UniProtKB-KW
Cellular response to DNA damage stimulus Source: UniProtKB-KW
Mitochondrial fragmentation involved in apoptotic process Source: UniProtKB
mRNA processing Source: UniProtKB-KW
Negative regulation of catalytic activity Source: UniProtKB
Negative regulation of cell growth Source: UniProtKB
Negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage Source: UniProtKB
Negative regulation of proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
Negative regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of apoptotic process Source: UniProtKB
Positive regulation of canonical Wnt signaling pathway Source: UniProtKB
Positive regulation of DNA damage checkpoint Source: UniProtKB
Regulation of cellular response to heat Source: Reactome
Regulation of circadian rhythm Source: UniProtKB
Regulation of DNA-templated transcription, elongation Source: UniProtKB
Regulation of protein deacetylation Source: UniProtKB
Regulation of protein stability Source: UniProtKB
Regulation of transcription, DNA-templated Source: GO_Central
Response to UV Source: UniProtKB
Rhythmic process Source: UniProtKB-KW
RNA splicing Source: UniProtKB
Wnt signaling pathway Source: UniProtKB-KW

Spindle; Cytoplasm; Nucleus. Recruited to chromatin, post-UV irradiation. Sequestered to the cytoplasm in the presence of MCC. Translocated to the cytoplasm during UV-induced apoptosis.

ATM/ATR-mediated phosphorylation at Thr-454 upon DNA damage promotes binding to SIRT1. Phosphorylation at Thr-454 promotes its sumoylation by switching the binding partner of CCAR2 from SENP1 to PIAS3.
Acetylation at Lys-112 and Lys-215 by KAT8 prevents inhibitory binding to SIRT1 and increases its deacetylase activity.
Genotoxic stress induces its sumoylation and sumoylation promotes the SIRT1-CCAR2 interaction which in turn inhibits SIRT1-mediated deacetylation of p53/TP53. Sumoylation leads to transcriptional activation of p53/TP53 by sequestering SIRT1 from p53/TP53. Desumoylated by SENP1.