Mouse Anti-CCDC115 Recombinant Antibody (4E9) (CBMAB-C6895-LY)

Name: Mouse Anti-CCDC115 Recombinant Antibody (4E9)
SKU: CBMAB-C6895-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

4E9

Application

ELISA, IHC-P, WB

Immunogen

CCDC115 (1 a.a. ~ 132 a.a) full-length recombinant protein with GST tag.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IHC-P	3 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

coiled-coil domain containing 115

Introduction

The protein encoded by this gene has been observed to localize to the endoplasmic reticulum (ER)-Golgi intermediate compartment (ERGIC) and coat protein complex I (COPI) vesicles in some human cells. The encoded protein shares some homology with the yeast V-ATPase assembly factor Vma22p, and the orthologous protein in mouse promotes cell proliferation and suppresses cell death. Defects in this gene are a cause of congenital disorder of glycosylation, type IIo in humans. [provided by RefSeq, Mar 2016]

Entrez Gene ID

84317

UniProt ID

Q96NT0

Alternative Names

Coiled-Coil Domain Containing 115; Coiled-Coil Domain-Containing Protein 115; CDG2O; CCP1;

Function

Accessory component of the proton-transporting vacuolar (V)-ATPase protein pump involved in intracellular iron homeostasis. In aerobic conditions, required for intracellular iron homeostasis, thus triggering the activity of Fe2+ prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation. Necessary for endolysosomal acidification and lysosomal degradation (PubMed:28296633).
May be involved in Golgi homeostasis (PubMed:26833332).

Biological Process

Cellular iron ion homeostasis Source: UniProtKB
Cellular response to increased oxygen levels Source: UniProtKB
Lysosomal lumen acidification Source: UniProtKB
Lysosomal protein catabolic process Source: UniProtKB
Vacuolar proton-transporting V-type ATPase complex assembly Source: InterPro

Cellular Location

Endosome; Lysosome; Endoplasmic reticulum; Endoplasmic reticulum-Golgi intermediate compartment; COPI-coated vesicle

Involvement in disease

Congenital disorder of glycosylation 2O (CDG2O): A form of congenital disorder of glycosylation, a genetically heterogeneous group of autosomal recessive, multisystem disorders caused by a defect in glycoprotein biosynthesis and characterized by under-glycosylated serum glycoproteins. Congenital disorders of glycosylation result in a wide variety of clinical features, such as defects in the nervous system development, psychomotor retardation, dysmorphic features, hypotonia, coagulation disorders, and immunodeficiency. The broad spectrum of features reflects the critical role of N-glycoproteins during embryonic development, differentiation, and maintenance of cell functions. CDG2O is characterized by hepatosplenomegaly, liver failure, hypotonia, and psychomotor disability.

Vuono, E. A., Ramirez-Medina, E., Berggren, K., Rai, A., Pruitt, S., Silva, E., ... & Borca, M. V. (2020). Swine host protein coiled-coil domain-containing 115 (CCDC115) interacts with classical swine fever virus structural glycoprotein E2 during virus replication. Viruses, 12(4), 388.

Sobh, A., Loguinov, A., Zhou, J., Jenkitkasemwong, S., Zeidan, R., El Ahmadie, N., ... & Vulpe, C. D. (2020). Genetic screens reveal CCDC115 as a modulator of erythroid iron and heme trafficking. American Journal of Hematology, 95(9), 1085-1098.

Girard, M., Poujois, A., Fabre, M., Lacaille, F., Debray, D., Rio, M., ... & Bruneel, A. (2018). CCDC115-CDG: A new rare and misleading inherited cause of liver disease. Molecular genetics and metabolism, 124(3), 228-235.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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