Rabbit Anti-CCNC Recombinant Antibody (EG515) (CBMAB-EN585-LY)

Name: Rabbit Anti-CCNC Recombinant Antibody (EG515)
SKU: CBMAB-EN585-LY
Rating: 5 (1 reviews)

See all CCNC antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

EG515

Application

WB, IP, IF, FC

Immunogen

Synthetic peptide corresponding to residues surrounding Pro263 of human cyclin C protein.

Host Species

Rabbit

Specificity

Human, Mouse

Antibody Isotype

IgG

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:1,000
IP	1:100
IF(ICC)	1:1,600-1:6,400
FC	1:100-1:400

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

HEPES, pH 7.5, 100 µg/ml BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Cyclin C

Introduction

CCNC (Cyclin C) is a Protein Coding gene. Among its related pathways are Signaling by NOTCH1 and Gene Expression. Gene Ontology (GO) annotations related to this gene include protein kinase binding. An important paralog of this gene is CCNK.

Entrez Gene ID

Human	892
Mouse	51813
Rat	114839

UniProt ID

Human	P24863
Mouse	Q62447
Rat	P39947

Alternative Names

Cyclin C; SRB11 Homolog; HSRB11; Cyclin-C; SRB11; CycC;

Function

Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Binds to and activates cyclin-dependent kinase CDK8 that phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex.

Biological Process

Positive regulation of transcription by RNA polymerase II Source: GO_Central
Regulation of transcription by RNA polymerase II Source: GO_Central
Regulation of transcription initiation from RNA polymerase II promoter Source: Reactome

Cellular Location

Nucleus

Tang, M., Pei, G., Su, D., Wang, C., Feng, X., Srivastava, M., ... & Chen, J. (2021). Genome-wide CRISPR screens reveal cyclin C as synthetic survival target of BRCA2. Nucleic acids research, 49(13), 7476-7491.

Lloyd, R. L., Urban, V., Muñoz-Martínez, F., Ayestaran, I., Thomas, J. C., de Renty, C., ... & Jackson, S. P. (2021). Loss of Cyclin C or CDK8 provides ATR inhibitor resistance by suppressing transcription-associated replication stress. Nucleic acids research, 49(15), 8665-8683.

Ponce, J. M., Coen, G., Spitler, K. M., Dragisic, N., Martins, I., Hinton Jr, A., ... & Grueter, C. E. (2020). Stress‐Induced Cyclin C Translocation Regulates Cardiac Mitochondrial Dynamics. Journal of the American Heart Association, 9(7), e014366.

Stieg, D. C., Cooper, K. F., & Strich, R. (2020). The extent of cyclin C promoter occupancy directs changes in stress-dependent transcription. Journal of Biological Chemistry, 295(48), 16280-16291.

Jezek, J., Wang, K., Yan, R., Di Cristofano, A., Cooper, K. F., & Strich, R. (2019). Synergistic repression of thyroid hyperplasia by cyclin C and Pten. Journal of cell science, 132(16), jcs230029.

Ježek, J., Smethurst, D. G., Stieg, D. C., Kiss, Z. A. C., Hanley, S. E., Ganesan, V., ... & Strich, R. (2019). Cyclin C: The story of a non-cycling cyclin. Biology, 8(1), 3.

Jezek, J., Chang, K. T., Joshi, A. M., & Strich, R. (2019). Mitochondrial translocation of cyclin C stimulates intrinsic apoptosis through Bax recruitment. EMBO reports, 20(9), e47425.

Stieg, D. C., Chang, K. T., Cooper, K. F., & Strich, R. (2019). Cyclin C regulated oxidative stress responsive transcriptome in Mus musculus embryonic fibroblasts. G3: Genes, Genomes, Genetics, 9(6), 1901-1908.

Ganesan, V., Willis, S. D., Chang, K. T., Beluch, S., Cooper, K. F., & Strich, R. (2019). Cyclin C directly stimulates Drp1 GTP affinity to mediate stress-induced mitochondrial hyperfission. Molecular biology of the cell, 30(3), 302-311.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Rabbit Anti-CCNC Recombinant Antibody (EG515)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry