Mouse Anti-CCNT1 Recombinant Antibody (CBXC-1770) (CBMAB-C3725-CQ)

Name: Mouse Anti-CCNT1 Recombinant Antibody (CBXC-1770)
SKU: CBMAB-C3725-CQ
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CCNT1 Recombinant Antibody (CBXC-1770) (CBMAB-C3725-CQ) in WB

The interactions of Myc-tagged chimeric proteins with the P-TEFb components CycT1 and CDK9. Each Myc-tagged protein in the cell lysate was pulled down by Myc antibody, and the interaction with P-TEFb was verified. ...View More

Kim, H. I., Kim, G. N., Yu, K. L., Park, S. H., & You, J. C. (2022). Identification of Novel Nucleocapsid Chimeric Proteins Inhibiting HIV-1 Replication. International Journal of Molecular Sciences, 23(20), 12340.

Mouse Anti-CCNT1 Recombinant Antibody (CBXC-1770) (CBMAB-C3725-CQ) in IF

Images of MDM nuclei showing INsfGFP fluorescence (green), EdU/vDNA (white), and CycT1 staining (red). Arrows point to the IN spots colocalized with EdU and P-TEFb complex; dashed circles indicate IN clusters that do not colocalize with EdU, P-TEFb, or both. ...View More

Francis, A. C., Marin, M., Prellberg, M. J., Palermino-Rowland, K., & Melikyan, G. B. (2020). HIV-1 uncoating and nuclear import precede the completion of reverse transcription in cell lines and in primary macrophages. Viruses, 12(11), 1234.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBXC-1770

Application

WB, IP, IF, ELISA, IHC-P

Immunogen

Amino acids 671-708 near the C-terminus of cyclin T1 of human origin.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:100-1:1,000
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Cyclin T1

Introduction

CCNT1 (Cyclin T1) is a Protein Coding gene. Diseases associated with CCNT1 include Human Immunodeficiency Virus Type 1. Among its related pathways are Formation of HIV elongation complex in the absence of HIV Tat and Gene Expression. Gene Ontology (GO) annotations related to this gene include chromatin binding and transcription regulatory region DNA binding. An important paralog of this gene is CCNT2.

Entrez Gene ID

904

UniProt ID

O60563

Alternative Names

Cyclin T1; Human Immunodeficiency Virus Type 1 (HIV-1) Expression (Elevated) 1; CYCT1; CDK9-Associated C-Type Protein; Cyclin C-Related Protein; MLLT10/CCNT1 Fusion;

Function

Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to productive elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II).
(Microbial infection) In case of HIV or SIV infections, binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). Serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes.

Biological Process

Cell cycle Source: UniProtKB-KW
Cell division Source: UniProtKB-KW
Negative regulation of mRNA polyadenylation Source: UniProtKB
Positive regulation by host of viral transcription Source: ParkinsonsUK-UCL
Positive regulation of DNA-templated transcription, elongation Source: GO_Central
Positive regulation of transcription by RNA polymerase II Source: UniProtKB
Positive regulation of viral transcription Source: Reactome
Protein phosphorylation Source: MGI
Regulation of cyclin-dependent protein serine/threonine kinase activity Source: ProtInc
Regulation of transcription by RNA polymerase II Source: GO_Central
snRNA transcription by RNA polymerase II Source: Reactome
Transcription by RNA polymerase II Source: Reactome
Transcription elongation from RNA polymerase II promoter Source: Reactome
Viral process Source: UniProtKB-KW

Cellular Location

Nucleus

Shiozaki, Y., Okamura, K., Kohno, S., Keenan, A. L., Williams, K., Zhao, X., ... & Miyazaki, M. (2018). The CDK9–cyclin T1 complex mediates saturated fatty acid–induced vascular calcification by inducing expression of the transcription factor CHOP. Journal of Biological Chemistry, 293(44), 17008-17020.

Zhou, J., Gao, G., Hou, P., Li, C. M., & Guo, D. (2017). Regulation of the Alternative Splicing and Function of Cyclin T1 by the Serine‐Arginine‐Rich Protein ASF/SF2. Journal of cellular biochemistry, 118(11), 4020-4032.

Forlani, G., Tosi, G., Turrini, F., Poli, G., Vicenzi, E., & Accolla, R. S. (2017). Tripartite motif-containing protein 22 interacts with class II transactivator and orchestrates its recruitment in nuclear bodies containing TRIM19/PML and cyclin T1. Frontiers in immunology, 8, 564.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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