Mouse Anti-CCT6A/B Recombinant Antibody (CBXC-0168) (CBMAB-C5570-CQ)

Name: Mouse Anti-CCT6A/B Recombinant Antibody (CBXC-0168)
SKU: CBMAB-C5570-CQ
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CCT6A/B Recombinant Antibody (CBXC-0168) (CBMAB-C5570-CQ) in WB

THOC3 expression in cells transfected with si-NC or si-CCT8/CCT6 shown by WB. ...View More

Yu, T., Zhang, Q., Yu, S. K., Nie, F. Q., Zhang, M. L., Wang, Q., & Lu, K. H. (2023). THOC3 interacts with YBX1 to promote lung squamous cell carcinoma progression through PFKFB4 mRNA modification. Cell Death & Disease, 14(7), 475. Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CCT6A/B Recombinant Antibody (CBXC-0168) (CBMAB-C5570-CQ) in IF

CCT/TRiC complex subunits beta and zeta (CCT2 and CCT6A) and proteasome subunit alpha 3 (PSMA3) interact with claudin-3 in MDCK-C7 cells. ...View More

Suarez-Artiles, L., Breiderhoff, T., Girardello, R., Gonschior, H., Rodius, S., Lesur, A., ... & Dittmar, G. (2022). Pan-claudin family interactome analysis reveals shared and specific interactions. Cell Reports, 41(6). Distributed under Open Access license CC BY 4.0, without modification.

Datasheet

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Basic Information

Host Animal

Mouse

Clone

CBXC-0168

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 342-531 mapping at the C-terminus of TCP-1 ζ of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Chaperonin Containing TCP1 Subunit 6B

Introduction

CCT6B (Chaperonin Containing TCP1 Subunit 6B) is a Protein Coding gene. Diseases associated with CCT6B include Pelvic Varices. Among its related pathways are Chaperonin-mediated protein folding and Cooperation of Prefoldin and TriC/CCT in actin and tubulin folding. Gene Ontology (GO) annotations related to this gene include unfolded protein binding and protein transporter activity. An important paralog of this gene is CCT6A.

Entrez Gene ID

10693

UniProt ID

Q92526

Function

Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis.

Biological Process

Protein folding Source: FlyBase
Toxin transport Source: Ensembl

Cellular Location

Cytoplasm

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Related Products

Mouse Anti-CCT6A/B Recombinant Antibody (CBYJT-1080) (CAT#: CBMAB-T0090-YJ)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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