Mouse Anti-CD3 Recombinant Antibody (HIT3a) (CBMAB-BC0047-LY)

Name: Mouse Anti-CD3 Recombinant Antibody (HIT3a)
SKU: CBMAB-BC0047-LY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CD3 Recombinant Antibody (HIT3a) (CBMAB-BC0047-LY) in FC

Flow cytometry analysis of decidual leukocytes. Comparison of typical subset-markers of NK cells (CD3-, CD56+, CD16+/-) and the non-typical dNK cells marker (CD9+). A-D) Representative FACS for two-color staining for decidual leukocytes. E-G) Representative FACS for three-color staining for decidual leukocytes. H-K) Representative FACS two-color staining for peripheral blood mononuclear cells (PBMC). L-M) Representative FACS three-color staining for PBMC. A, E, H, L) negative cells; B, I) upper left quadrants (UL): CD56+ CD3- cells; C, J) UL: CD56+ CD16- cells; upper right quadrants (UR): CD56+ CD16+ cells; D, K) UR: CD56+CD9+ cells. F, M) R1 and R2, gate for CD3- cells; G, N) CD56+CD9+/- expression on CD3- cells. ...View More

Sánchez-Rodríguez, E. N., Nava-Salazar, S., Mendoza-Rodríguez, C. A., Moran, C., Romero-Arauz, J. F., Ortega, E., ... & Cerbón, M. (2011). Persistence of decidual NK cells and KIR genotypes in healthy pregnant and preeclamptic women: a case-control study in the third trimester of gestation. Reproductive Biology and Endocrinology, 9, 1-9. Distributed under Open Access license CC BY 2.0, without modification.

Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

HIT3a

Application

IF, FC

Immunogen

CD3 and CD3/TCR complex of human origin.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Cluster Of Differentiation 3

Alternative Names

CD3

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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