Rat Anti-CD300E Recombinant Antibody (233810) (CBMAB-C0694-LY)

Name: Rat Anti-CD300E Recombinant Antibody (233810)
SKU: CBMAB-C0694-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rat

Clone

233810

Application

WB, FC

Immunogen

Y3 rat myeloid cell line transfected with human CD300e/LMIR6.

Host Species

Rat

Specificity

Human

Antibody Isotype

IgG2a

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1 µg/ml
FC	2.5 µg/10^6 cells

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

PBS, Trehalose

Preservative

None

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

CD300e Molecule

Introduction

CD300E (CD300e Molecule) is a Protein Coding gene.
Diseases associated with CD300E include Tungiasis.
Among its related pathways are Innate Immune System and Class I MHC mediated antigen processing and presentation.

An important paralog of this gene is CD300C.

Entrez Gene ID

342510

UniProt ID

Q496F6

Function

Probably acts as an activating receptor.

Biological Process

Innate immune response Source: Reactome
Regulation of immune response Source: Reactome

Cellular Location

Cell membrane

Topology

Extracellular: 18-173
Helical: 174-194
Cytoplasmic: 195-205

PTM

N-glycosylated.

Chen, K., Silveira, P., Lo, T. H., Hart, D., & Clark, G. (2018). Characterizing the function of the CD300e immunoregulatory molecule.

Pagliari, M., Munari, F., Toffoletto, M., Lonardi, S., Chemello, F., Codolo, G., ... & Cagnin, S. (2017). Helicobacter pylori affects the antigen presentation activity of macrophages modulating the expression of the immune receptor CD300E through miR-4270. Frontiers in immunology, 8, 1288.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Human Anti-CD300E Recombinant Antibody (CBFYC-0455) (CAT#: CBMAB-C0501-FY)

Human Recombinant CD300E protein, hFc Tag (CAT#: V2LY-0526-LY2794)

Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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