Mouse Anti-CD46 Recombinant Antibody (CBT2832) (V2LY-0625-LY1046)

Name: Mouse Anti-CD46 Recombinant Antibody (CBT2832)
SKU: V2LY-0625-LY1046
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-CD46 Recombinant Antibody (CBT2832) (V2LY-0625-LY1046) in ELISA

Mouse Anti-CD46 Recombinant Antibody (CBT2832) (V2LY-0625-LY1046) in WB

Mouse Anti-CD46 Recombinant Antibody (CBT2832) (V2LY-0625-LY1046) in FC

Mouse Anti-CD46 Recombinant Antibody (CBT2832) (V2LY-0625-LY1046) in IHC

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBT2832

Application

IHC, FC

Immunogen

Purified recombinant fragment of human CD46 (AA: extra 35-179) expressed in E. Coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
IHC-P	1:200-1:1,000
FC	1:200-1:400
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

CD46 Molecule

Entrez Gene ID

4179

UniProt ID

P15529

Function

Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Also acts as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity.
(Microbial infection) A number of viral and bacterial pathogens seem to bind MCP in order to exploit its immune regulation property and directly induce an immunosuppressive phenotype in T-cells.
(Microbial infection) Acts as a receptor for Adenovirus subgroup B2 and Ad3.
(Microbial infection) Acts as a receptor for cultured Measles virus.
(Microbial infection) Acts as a receptor for Herpesvirus 6/HHV-6.
(Microbial infection) May act as a receptor for pathogenic bacteria Neisseria and Streptococcus pyogenes (PubMed:7708671, PubMed:9379894, PubMed:11260136, PubMed:11971006).

Biological Process

Adaptive immune response Source: UniProtKB
Complement activation, classical pathway Source: UniProtKB-KW
Innate immune response Source: UniProtKB-KW
Negative regulation of gene expression Source: UniProtKB
Positive regulation of gene expression Source: UniProtKB
Positive regulation of interleukin-10 production Source: UniProtKB
Positive regulation of memory T cell differentiation Source: UniProtKB
Positive regulation of regulatory T cell differentiation Source: UniProtKB
Positive regulation of T cell proliferation Source: UniProtKB
Positive regulation of transforming growth factor beta production Source: UniProtKB
Regulation of complement activation Source: Reactome
Regulation of Notch signaling pathway Source: UniProtKB
Sequestering of extracellular ligand from receptor Source: UniProtKB
Single fertilization Source: UniProtKB-KW
T cell mediated immunity Source: UniProtKB

Cellular Location

Acrosome inner membrane. Inner acrosomal membrane of spermatozoa. Internalized upon binding of Measles virus, Herpesvirus 6 or Neisseria gonorrhoeae, which results in an increased susceptibility of infected cells to complement-mediated injury. In cancer cells or cells infected by Neisseria, shedding leads to a soluble peptide.

Involvement in disease

Hemolytic uremic syndrome atypical 2 (AHUS2): Disease susceptibility is associated with variants affecting the gene represented in this entry. Other genes may play a role in modifying the phenotype. Patients with CD46 mutations seem to have an overall better prognosis compared to patients carrying CFH mutations. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease.

Topology

Extracellular: 35-343
Helical: 344-366
Cytoplasmic: 367-392

PTM

N-glycosylated on Asn-83; Asn-114 and Asn-273 in most tissues, but probably less N-glycosylated in testis. N-glycosylation on Asn-114 and Asn-273 is required for cytoprotective function. N-glycosylation on Asn-114 is required for Measles virus binding. N-glycosylation on Asn-273 is required for Neisseria binding. N-glycosylation is not required for human adenovirus binding.
Extensively O-glycosylated in the Ser/Thr-rich domain. O-glycosylation is required for Neisseria binding but not for Measles virus or human adenovirus binding.
In epithelial cells, isoforms B/D/F/H/J/L/3 are phosphorylated by YES1 in response to infection by Neisseria gonorrhoeae; which promotes infectivity. In T-cells, these isoforms may be phosphorylated by LCK.

Wang, S., Li, J., Hua, J., Su, Y., Beckford-Vera, D. R., Zhao, W., ... & He, J. (2021). Molecular imaging of prostate cancer targeting CD46 using immunoPET. Clinical Cancer Research, 27(5), 1305-1315.

Wang, S., Su, Y., Escobar, B. P., Steri, V., Hann, B., Tang, R., ... & Flavell, R. (2021). Molecular Imaging of Multiple Myeloma Targeting CD46 Using ImmunoPET.

Muñoz-Alía, M. Á., Nace, R. A., Tischer, A., Zhang, L., Bah, E. S., Auton, M., & Russell, S. J. (2021). MeV-Stealth: A CD46-specific oncolytic measles virus resistant to neutralization by measles-immune human serum. PLoS pathogens, 17(2), e1009283.

Schack, V. R., Rossen, L. S., Ekebjærg, C. C., Thuesen, K. K. H., Bundgaard, B., & Höllsberg, P. (2021). The tetraspanin protein CD9 modulates infection with human herpesvirus 6A and 6B in a CD46-dependent manner. Journal of Virology, 95(8), e02259-20.

Feng, Y., Yi, C., Liu, X., Qu, L., Su, W., Shu, T., ... & Feng, L. (2020). Human desmoglein-2 and human CD46 mediate human adenovirus type 55 infection, but human desmoglein-2 plays the major roles. Journal of virology, 94(17), e00747-20.

Riedel, C., Chen, H. W., Reichart, U., Lamp, B., Laketa, V., & Rümenapf, T. (2020). Real time analysis of bovine viral diarrhea virus (BVDV) infection and its dependence on bovine CD46. Viruses, 12(1), 116.

Sajjadi, S., Shirode, A., Vaidya, S. R., & Cherian, S. S. (2019). Molecular mechanism by which residues at position 481 and 546 of measles virus hemagglutinin protein define CD46 receptor binding using a molecular docking approach. Computational biology and chemistry, 80, 384-389.

Liszewski, M. K., & Kemper, C. (2019). Complement in motion: the evolution of CD46 from a complement regulator to an orchestrator of normal cell physiology. Journal of immunology (Baltimore, Md.: 1950), 203(1), 3.

Ni Choileain, S., Hay, J., Thomas, J., Williams, A., Vermeren, M. M., Benezech, C., ... & Astier, A. L. (2017). TCR-stimulated changes in cell surface CD46 expression generate type 1 regulatory T cells. Science signaling, 10(502), eaah6163.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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