Mouse Anti-CDH18 Recombinant Antibody (6F7) (CBMAB-C8285-LY)

Name: Mouse Anti-CDH18 Recombinant Antibody (6F7)
SKU: CBMAB-C8285-LY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CDH18 Recombinant Antibody (6F7) (CBMAB-C8285-LY) in IF

The responder cell line LS8817 was unmanipulated (control), transduced with a PDLIM7 knockdown lentiviral vector (shP2), or a vector containing Cas9 and a vector containing a guide RNA against CDH18 (KO1 and KO2). PDLIM7 and pan-cadherin were visualized by co-immunofluorescence (n = 2). ...View More

Klein, M. E., Dickson, M. A., Antonescu, C., Qin, L. X., Dooley, S. J., Barlas, A., ... & Tap, W. D. (2018). PDLIM7 and CDH18 regulate the turnover of MDM2 during CDK4/6 inhibitor therapy-induced senescence. Oncogene, 37(37), 5066-5078.

Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CDH18 Recombinant Antibody (6F7) (CBMAB-C8285-LY) in IHC

Patient tumor samples were obtained during surgery and formalin fixed and paraffin embedded. After antigen retrieval, IHC was performed using a CDH18-specific antibody. Hematoxylin and eosin was used as a counterstain. Samples were blinded during analysis. Representative images are shown from two patients with dedifferentiated histologies, a CDH18-negative tumor (left) and a CDH18-positive tumor (right). ...View More

Distributed under Open Access license CC BY 4.0, without modification.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

6F7

Application

ELISA, IHC, WB

Immunogen

Partial recombinant corresponding to aa467-576 from human CDH18.

Host Species

Mouse

Specificity

Human, Mouse

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IHC	3 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Cadherin 18

Introduction

CDH18 (Cadherin 18) is a Protein Coding gene. Among its related pathways are ERK Signaling and CDK-mediated phosphorylation and removal of Cdc6. Gene Ontology (GO) annotations related to this gene include calcium ion binding. An important paralog of this gene is CDH7.

Entrez Gene ID

1016

UniProt ID

Q13634

Alternative Names

Cadherin 18; Cadherin 18, Type 2; Cadherin-14; CDH14; Cadherin-Like 24; Cadherin-18; Ey-Cadherin; CDH14L; CDH24;

Function

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.

Biological Process

Adherens junction organization Source: GO_Central
Calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules Source: GO_Central
Cell-cell adhesion via plasma-membrane adhesion molecules Source: GO_Central
Cell-cell junction assembly Source: GO_Central
Cell morphogenesis Source: GO_Central
Homophilic cell adhesion via plasma membrane adhesion molecules Source: InterPro
Multicellular organism development Source: GO_Central

Cellular Location

Cell membrane

Topology

Extracellular: 54-608
Helical: 609-636
Cytoplasmic: 637-790

Mamoor, S. (2020). Cadherin 18 (Cadherin 14) is differentially expressed in the tumors of breast cancer patients treated with trastuzumab.

Schlicht, K., Nyczka, P., Caliebe, A., Freitag-Wolf, S., Claringbould, A., Franke, L., ... & Krawczak, M. (2019). The metabolic network coherence of human transcriptomes is associated with genetic variation at the cadherin 18 locus. Human genetics, 138(4), 375-388.

Bai, Y. H., Zhan, Y. B., Yu, B., Wang, W. W., Wang, L., Zhou, J. Q., ... & Liu, X. Z. (2018). A novel tumor-suppressor, CDH18, inhibits glioma cell invasiveness via UQCRC2 and correlates with the prognosis of glioma patients. Cellular Physiology and Biochemistry, 48(4), 1755-1770.

Chen, C. P., Chang, S. Y., Lin, C. J., Chern, S. R., Wu, P. S., Chen, S. W., ... & Wang, W. (2018). Prenatal diagnosis of a familial 5p14. 3-p14. 1 deletion encompassing CDH18, CDH12, PMCHL1, PRDM9 and CDH10 in a fetus with congenital heart disease on prenatal ultrasound. Taiwanese Journal of Obstetrics and Gynecology, 57(5), 734-738.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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