Mouse Anti-CDK2AP1 Recombinant Antibody (CBXC-1742) (CBMAB-C3642-CQ)

Name: Mouse Anti-CDK2AP1 Recombinant Antibody (CBXC-1742)
SKU: CBMAB-C3642-CQ
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CDK2AP1 Recombinant Antibody (CBXC-1742) (CBMAB-C3642-CQ) in WB

Western blot analysis of the loss-of-function consequences of miR-21-5p inhibition on the CDK2AP1-deficient cell line panel. The SCCs cell lines were transfected with the MIRZIP lentiviral vectors targeting either miR-21-5p (mirZip-21) or a scrambled control sequence (miR-Zip-SCR). Experiments were performed in triplicate and β-actin (BACT) was employed as loading control. ...View More

Stabile, R., Cabezas, M. R., Verhagen, M. P., Tucci, F. A., van den Bosch, T. P., De Herdt, M. J., ... & Fodde, R. (2023). The deleted in oral cancer (DOC1 aka CDK2AP1) tumor suppressor gene is downregulated in oral squamous cell carcinoma by multiple microRNAs. Cell Death & Disease, 14(5), 337.

Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CDK2AP1 Recombinant Antibody (CBXC-1742) (CBMAB-C3642-CQ) in IF

Representative images of CDK2AP1-positive (left) and -negative (right) TMA tumor cores featuring low and high expression of miR-21-5p, respectively. Next to the microscope images, digital reconstructions with tumor cells as red dots, and stromal cells as gray dots are depicted. In the lower panels, higher magnifications of the corresponding inlets are shown. ...View More

Distributed under Open Access license CC BY 4.0, without modification.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBXC-1742

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 20-76 mapping within an internal region of CDK2AP1 of human.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Cyclin Dependent Kinase 2 Associated Protein 1

Introduction

CDK2AP1 (Cyclin Dependent Kinase 2 Associated Protein 1) is a Protein Coding gene. Diseases associated with CDK2AP1 include Oral Cancer. Gene Ontology (GO) annotations related to this gene include DNA polymerase binding. An important paralog of this gene is CDK2AP2.

Entrez Gene ID

8099

UniProt ID

O14519

Alternative Names

Cyclin Dependent Kinase 2 Associated Protein 1; CDK2-Associated Protein 1; Putative Oral Cancer Suppressor; Deleted In Oral Cancer 1; Doc-1; DOC1; Cyclin-Dependent Kinase 2-Associated Protein 1;

Function

Specific inhibitor of the cell-cycle kinase CDK2.

Biological Process

Cell cycle Source: UniProtKB-KW
DNA-dependent DNA replication Source: UniProtKB
Positive regulation of protein phosphorylation Source: UniProtKB

Cellular Location

Cytosol; Nucleoplasm; Nucleus; Perinuclear region of cytoplasm

PTM

Phosphorylated in vitro by IKBKE at Ser-46.

Sahu, S. N., Satpathy, S. S., Mohanty, C., & Pattanayak, S. K. (2021). Computational study to evaluate the potency of phytochemicals in Boerhavia diffusa and the impact of point mutation on cyclin-dependent kinase 2-associated protein 1. Journal of Biomolecular Structure and Dynamics, 1-15.

Modzelewski, A. J., Shao, W., Chen, J., Lee, A., Qi, X., Noon, M., ... & He, L. (2021). A mouse-specific retrotransposon drives a conserved Cdk2ap1 isoform essential for development. Cell, 184(22), 5541-5558.

Li, Q. S., & De Muynck, L. (2021). Differentially expressed genes in Alzheimer’s disease highlighting the roles of microglia genes including OLR1 and astrocyte gene CDK2AP1. Brain, Behavior, & Immunity-Health, 13, 100227.

Wang, Z., Xiang, Z., Zhu, T., Chen, J., Zhong, M. Z., Huang, J., ... & Zhou, W. B. (2020). Cathepsin L interacts with CDK2‑AP1 as a potential predictor of prognosis in patients with breast cancer. Oncology letters, 19(1), 167-176.

Gera, R., Mokbel, L., Jiang, W. G., & Mokbel, K. (2018). mRNA expression of CDK2AP1 in human breast cancer: Correlation with clinical and pathological parameters. Cancer genomics & proteomics, 15(6), 447-452.

Alsayegh, K. N., Sheridan, S. D., Iyer, S., & Rao, R. R. (2018). Knockdown of CDK2AP1 in human embryonic stem cells reduces the threshold of differentiation. PloS one, 13(5), e0196817.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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