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Mouse Anti-CDK5R1 Recombinant Antibody (CBLNP-193) (CBMAB-1342-CN)

This product is a mouse antibody that recognizes CDK5R1 of human. The antibody CBLNP-193 can be used for immunoassay techniques such as: ELISA, WB, IHC-P.
See all CDK5R1 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse
Clone
CBLNP-193
Antibody Isotype
IgG2a
Application
ELISA, WB, IHC-P

Basic Information

Immunogen
Recombinant tagged fragment: CRDVISSEVG SDHELQAVLL TCLYLSYSYM GNEISYPLKP FLVESCKEAF WDRCLSVINL MSSKMLQINA DPHYFTQVFS DLKNESGQED KKRLLLGLDR, corresponding to aa. 208-308 of Human p35
Specificity
Human, Mouse
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2
Concentration
0.5 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Cyclin Dependent Kinase 5 Regulatory Subunit 1
Introduction
The protein encoded by this gene (p35) is a neuron-specific activator of cyclin-dependent kinase 5 (CDK5); the activation of CDK5 is required for proper development of the central nervous system. The p35 form of this protein is proteolytically cleaved by calpain, generating a p25 form. P25 deregulates CDK5 activity by prolonging its activation and changing its cellular location. p35 is a neuron specific activator of CDK5. The complex p35/CDK5 is required for neurite outgrowth and cortical lamination.
Entrez Gene ID
Human8851
Mouse12569
UniProt ID
HumanQ15078
MouseP61809
Alternative Names
p23; p25; p35; CDK5R; NCK5A; CDK5P35; p35nck5a
Function
p35 is a neuron specific activator of CDK5. The complex p35/CDK5 is required for neurite outgrowth and cortical lamination. Involved in dendritic spine morphogenesis by mediating the EFNA1-EPHA4 signaling. Activator of TPKII. The complex p35/CDK5 participates in the regulation of the circadian clock by modulating the function of CLOCK protein: phosphorylates CLOCK at 'Thr-451' and 'Thr-461' and regulates the transcriptional activity of the CLOCK-ARNTL/BMAL1 heterodimer in association with altered stability and subcellular distribution.
Biological Process
Axonal fasciculation Source: UniProtKB
Axon guidance Source: UniProtKB
Brain development Source: UniProtKB
Cerebellum development Source: Ensembl
Embryo development ending in birth or egg hatching Source: Ensembl
Ephrin receptor signaling pathway Source: UniProtKB
G protein-coupled acetylcholine receptor signaling pathway Source: UniProtKB
Hippocampus development Source: Ensembl
Ionotropic glutamate receptor signaling pathway Source: UniProtKB
Layer formation in cerebral cortex Source: Ensembl
Microtubule cytoskeleton organization Source: ARUK-UCL
Negative regulation of axon extension Source: Ensembl
Negative regulation of transcription, DNA-templated Source: DFLAT
Neuron cell-cell adhesion Source: UniProtKB
Neuron differentiation Source: UniProtKB
Neuron migration Source: UniProtKB
Neuron projection development Source: UniProtKB
Peptidyl-serine phosphorylation Source: UniProtKB
Peptidyl-threonine phosphorylation Source: UniProtKB
Positive regulation of cell cycle arrest Source: Ensembl
Positive regulation of MHC class II biosynthetic process Source: ARUK-UCL
Positive regulation of microtubule polymerization Source: ARUK-UCL
Positive regulation of neuron apoptotic process Source: UniProtKB
Positive regulation of protein targeting to membrane Source: Ensembl
Positive regulation of signaling receptor activity Source: ARUK-UCL
Regulation of actin cytoskeleton organization Source: Ensembl
Regulation of cyclin-dependent protein serine/threonine kinase activity Source: ProtInc
Regulation of dendritic spine morphogenesis Source: UniProtKB
Regulation of macroautophagy Source: ParkinsonsUK-UCL
Regulation of neuron differentiation Source: UniProtKB
Regulation of signal transduction by p53 class mediator Source: Reactome
Regulation of synaptic vesicle cycle Source: Ensembl
Rhythmic process Source: UniProtKB-KW
Serine phosphorylation of STAT protein Source: Ensembl
Superior olivary nucleus maturation Source: Ensembl
Cellular Location
Cyclin-dependent kinase 5 activator 1, p35: Cell membrane; Neuron projection. In the primary cortical neurons, p35 is present in the peripheries and nerve terminals.
Cyclin-dependent kinase 5 activator 1, p25: Nucleus; Perinuclear region; Perikaryon. The conversion of p35 to p25 relocalizes the protein from the cell periphery to the cytoplasm, in nuclear and perinuclear regions. In the primary cortical neurons, p25 is primarily concentrated in the cell soma and is largely absent from neurites.
PTM
The p35 form is proteolytically cleaved by calpain, giving rise to the p25 form. P35 has a 5 to 10 fold shorter half-life compared to p25. The conversion results in deregulation of the CDK5 kinase: p25/CDK5 kinase displays an increased and altered tau phosphorylation in comparison to the p35/CDK5 kinase in vivo (By similarity).
Myristoylated. A proper myristoylation signal is essential for the proper distribution of p35.
Ubiquitinated. Degradation of p35 by proteasome results in down-regulation of CDK5 activity. During this process, CDK5 phosphorylates p35 and induces its ubiquitination and subsequent degradation.
Phosphorylation at Ser-8 and Thr-138 by CDK5 prevents calpain-mediated proteolysis.

Zhou, J., Chow, H. M., Liu, Y., Wu, D., Shi, M., Li, J., ... & Zhang, J. (2020). Cyclin-Dependent Kinase 5–Dependent BAG3 Degradation Modulates Synaptic Protein Turnover. Biological psychiatry, 87(8), 756-769.

Man, A., Slevin, M., Petcu, E., & Fraefel, C. (2019). The cyclin-dependent kinase 5 inhibitor peptide inhibits herpes simplex virus type 1 replication. Scientific reports, 9(1), 1-8.

Ruiz de Porras, V., Bystrup, S., Cabrero-de Las Heras, S., Musulén, E., Palomero, L., Alonso, M. H., ... & Martinez-Balibrea, E. (2019). Tumor expression of cyclin-dependent kinase 5 (Cdk5) is a prognostic biomarker and predicts outcome of oxaliplatin-treated metastatic colorectal cancer patients. Cancers, 11(10), 1540.

Spreafico, M., Grillo, B., Rusconi, F., Battaglioli, E., & Venturin, M. (2018). Multiple layers of CDK5R1 regulation in Alzheimer’s disease implicate long non-coding RNAs. International journal of molecular sciences, 19(7), 2022.

Lintas, C., Sacco, R., Tabolacci, C., Brogna, C., Canali, M., Picinelli, C., ... & Persico, A. M. (2018). An Interstitial 17q11. 2 de novo Deletion Involving the CDK5R1 Gene in a High-Functioning Autistic Patient. Molecular syndromology, 9(5), 247-252.

Wei, J., Marangoni, R. G., Fang, F., Wang, W., Huang, J., Distler, J. H., & Varga, J. (2018). The non-neuronal cyclin-dependent kinase 5 is a fibrotic mediator potentially implicated in systemic sclerosis and a novel therapeutic target. Oncotarget, 9(12), 10294.

Qian, T., Wang, X., Wang, Y., Wang, P., Liu, Q., Liu, J., & Yi, S. (2018). Novel miR-sc4 regulates the proliferation and migration of Schwann cells by targeting Cdk5r1. Molecular and cellular biochemistry, 447(1), 209-215.

Farina, F. M., Inguscio, A., Kunderfranco, P., Cortesi, A., Elia, L., & Quintavalle, M. (2017). MicroRNA-26a/cyclin-dependent kinase 5 axis controls proliferation, apoptosis and in vivo tumor growth of diffuse large B-cell lymphoma cell lines. Cell death & disease, 8(6), e2890-e2890.

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For research use only. Not intended for any clinical use.

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