Mouse Anti-CDK5RAP1 Recombinant Antibody (CBLNC-247) (CBMAB-1344-CN)

Name: Mouse Anti-CDK5RAP1 Recombinant Antibody (CBLNC-247)
SKU: CBMAB-1344-CN
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBLNC-247

Application

ELISA, WB, IP, IF

Immunogen

Amino acids 200-497 mapping at the C-terminus of CDK5RAP1 of human.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

CDK5 Regulatory Subunit Associated Protein 1

Introduction

This gene encodes a regulator of cyclin-dependent kinase 5 activity. The protein has also been reported to modify RNA by adding a methylthio-group and may thus have a dual function as an RNA methylthiotransferase and as an inhibitor of cyclin-dependent kinase 5 activity. Specifically inhibits CDK5 activation by CDK5R1.

Entrez Gene ID

51654

UniProt ID

Q96SZ6

Alternative Names

C42; CGI-05; HSPC167; C20orf34

Function

Methylthiotransferase that catalyzes the conversion of N6-(dimethylallyl)adenosine (i6A) to 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 (adjacent to the 3'-end of the anticodon) of four mitochondrial DNA-encoded tRNAs (Ser(UCN), Phe, Tyr and Trp) (PubMed:22422838, PubMed:25738458, PubMed:28981754).
Essential for efficient and highly accurate protein translation by the ribosome (PubMed:22422838, PubMed:25738458, PubMed:28981754).
Specifically inhibits CDK5 activation by CDK5R1 (PubMed:11882646).
Essential for efficient mitochondrial protein synthesis and respiratory chain; shows pathological consequences in mitochondrial disease (PubMed:25738458).

Biological Process

Brain development Source: UniProtKB
Mitochondrial tRNA modification Source: Ensembl
Negative regulation of cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB
Positive regulation of cyclin-dependent protein serine/threonine kinase activity Source: Ensembl
Positive regulation of mitochondrial translation Source: Ensembl
Positive regulation of translational fidelity Source: Ensembl
Regulation of neuron differentiation Source: UniProtKB

Cellular Location

Mitochondrion

Miwa, T., Wei, F. Y., & Tomizawa, K. (2021). Cdk5 regulatory subunit-associated protein 1 knockout mice show hearing loss phenotypically similar to age-related hearing loss. Molecular brain, 14(1), 1-16.

Yamamoto, T., Fujimura, A., Fan-Yan, W., Kai, K., Takezaki, T., Ohta, K., ... & Mukasa, A. (2019). BOT-02 2-METHYLTHIO MODIFICATION OF N6-ISOPENTENYLADENOSINE IN MITOCHONDRIAL TRNAS BY CDK5RAP1 PROMOTES THE MAINTENANCE OF GLIOMA-INITIATING CELLS. Neuro-oncology Advances, 1(Suppl 2), ii12.

Yamamoto, T., Fujimura, A., Wei, F. Y., Shinojima, N., Kuroda, J. I., Mukasa, A., & Tomizawa, K. (2019). 2-methylthio conversion of N6-isopentenyladenosine in mitochondrial tRNAs by CDK5RAP1 promotes the maintenance of glioma-initiating cells. Iscience, 21, 42-56.

Zhang, C., Sha, H., Peng, Y., Wang, Y., Liu, C., & Zhou, X. (2019). PiRNA-DQ541777 contributes to neuropathic pain via targeting Cdk5rap1. Journal of Neuroscience, 39(45), 9028-9039.

Xiong, J., Wang, Y., Gu, Y., Xue, Y., Dang, L., & Li, Y. (2018). CDK5RAP1 targeting NF-κB signaling pathway in human malignant melanoma A375 cell apoptosis. Oncology letters, 15(4), 4767-4774.

Fakruddin, M., Wei, F. Y., Emura, S., Matsuda, S., Yasukawa, T., Kang, D., & Tomizawa, K. (2017). Cdk5rap1-mediated 2-methylthio-N 6-isopentenyladenosine modification is absent from nuclear-derived RNA species. Nucleic acids research, 45(20), 11954-11961.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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