Mouse Anti-CDK6 Recombinant Antibody (CBFYC-1613) (CBMAB-C1672-FY)

Name: Mouse Anti-CDK6 Recombinant Antibody (CBFYC-1613)
SKU: CBMAB-C1672-FY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CDK6 Recombinant Antibody (CBFYC-1613) (CBMAB-C1672-FY) in WB

Western blotting of cyclinD1, Cdk4, and Cdk6 protein levels. ...View More

Yun, H. M., Kwon, H. S., Lee, J. Y., & Park, K. R. (2024). Vitexicarpin Induces Apoptosis and Inhibits Metastatic Properties via the AKT-PRAS40 Pathway in Human Osteosarcoma. International Journal of Molecular Sciences, 25(7), 3582.

Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CDK6 Recombinant Antibody (CBFYC-1613) (CBMAB-C1672-FY) in WB

Western blotting, showing expression of important proteins associated with the G0-G1 phase. ...View More

Ren, G., Li, H., Hong, D., Hu, F., Jin, R., Wu, S., ... & Huang, H. (2023). LINC00955 suppresses colorectal cancer growth by acting as a molecular scaffold of TRIM25 and Sp1 to Inhibit DNMT3B-mediated methylation of the PHIP promoter. BMC cancer, 23(1), 898.

Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CDK6 Recombinant Antibody (CBFYC-1613) (CBMAB-C1672-FY) in WB

Representative blots for CDK4, CDK6, Rb, p-Rb, CDK1, Cyclin A2, and mTOR. ...View More

Wang, Y., Li, Y., Liu, D., Zheng, D., Li, X., Li, C., ... & Xu, J. (2023). A Potential Anti-Glioblastoma Compound LH20 Induces Apoptosis and Arrest of Human Glioblastoma Cells via CDK4/6 Inhibition. Molecules, 28(13), 5047.

Distributed under Open Access license CC BY 4.0, without modification.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYC-1613

Application

ELISA, WB, IP, IF, IHC-P

Immunogen

Amino acids 1-326 representing full length Cdk6 of human.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Cyclin Dependent Kinase 6

Introduction

The activation of cdk6 kinase occurs during mid-G1 in phytohemagglutinin-stimulated T cells, well prior to the activation of cdk2 kinase.

Entrez Gene ID

1021

UniProt ID

Q00534

Alternative Names

Cyclin Dependent Kinase 6; Serine/Threonine-Protein Kinase PLSTIRE; Cell Division Protein Kinase 6; EC 2.7.11.22; Cyclin-Dependent Kinase 6; EC 2.7.11; PLSTIRE; MCPH12; CDKN6

Function

Serine/threonine-protein kinase involved in the control of the cell cycle and differentiation; promotes G1/S transition. Phosphorylates pRB/RB1 and NPM1. Interacts with D-type G1 cyclins during interphase at G1 to form a pRB/RB1 kinase and controls the entrance into the cell cycle. Involved in initiation and maintenance of cell cycle exit during cell differentiation; prevents cell proliferation and regulates negatively cell differentiation, but is required for the proliferation of specific cell types (e.g. erythroid and hematopoietic cells). Essential for cell proliferation within the dentate gyrus of the hippocampus and the subventricular zone of the lateral ventricles. Required during thymocyte development. Promotes the production of newborn neurons, probably by modulating G1 length. Promotes, at least in astrocytes, changes in patterns of gene expression, changes in the actin cytoskeleton including loss of stress fibers, and enhanced motility during cell differentiation. Prevents myeloid differentiation by interfering with RUNX1 and reducing its transcription transactivation activity, but promotes proliferation of normal myeloid progenitors. Delays senescence. Promotes the proliferation of beta-cells in pancreatic islets of Langerhans. May play a role in the centrosome organization during the cell cycle phases (PubMed:23918663).

Biological Process

Astrocyte development Source: UniProtKB
Cell cycle arrest Source: UniProtKB
Cell dedifferentiation Source: BHF-UCL
Cell division Source: UniProtKB-KW
Dentate gyrus development Source: UniProtKB
G1/S transition of mitotic cell cycle Source: UniProtKB
Generation of neurons Source: UniProtKB
Gliogenesis Source: BHF-UCL
Lateral ventricle development Source: UniProtKB
Negative regulation of cell cycle Source: UniProtKB
Negative regulation of cell differentiation Source: UniProtKB
Negative regulation of cell population proliferation Source: UniProtKB
Negative regulation of cellular senescence Source: UniProtKB
Negative regulation of epithelial cell proliferation Source: BHF-UCL
Negative regulation of G1/S transition of mitotic cell cycle Source: Reactome
Negative regulation of monocyte differentiation Source: UniProtKB
Negative regulation of myeloid cell differentiation Source: UniProtKB
Negative regulation of osteoblast differentiation Source: BHF-UCL
Positive regulation of cell-matrix adhesion Source: BHF-UCL
Positive regulation of fibroblast proliferation Source: BHF-UCL
Protein phosphorylation Source: BHF-UCL
Regulation of cell cycle Source: GO_Central
Regulation of cell motility Source: UniProtKB
Regulation of erythrocyte differentiation Source: BHF-UCL
Regulation of gene expression Source: BHF-UCL
Response to virus Source: UniProtKB
Type B pancreatic cell development Source: UniProtKB

Cellular Location

Centrosome; Nucleus; Cytoplasm; Ruffle. Localized to the ruffling edge of spreading fibroblasts. Kinase activity only in nucleus. Localized to the cytosol of neurons and showed prominent staining around either side of the nucleus (By similarity). Present in the cytosol and in the nucleus in interphase cells and at the centrosome during mitosis from prophase to telophase (PubMed:23918663).

Involvement in disease

Microcephaly 12, primary, autosomal recessive (MCPH12): A form of microcephaly, a disease defined as a head circumference more than 3 standard deviations below the age-related mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small.

PTM

Thr-177 phosphorylation and Tyr-24 dephosphorylation promotes kinase activity.

Zhou, H., Jia, X., Yang, F., & Shi, P. (2021). miR-148a-3p suppresses the progression of acute myeloid leukemia via targeting cyclin-dependent kinase 6 (CDK6). Bioengineered, 12(1), 4508-4519.

Luo, X., Zhao, Y., Tang, P., Du, X., Li, F., Wang, Q., ... & He, J. (2021). Discovery of new small-molecule cyclin-dependent kinase 6 inhibitors through computational approaches. Molecular Diversity, 25(1), 367-382.

Yousuf, M., Shamsi, A., Queen, A., Shahbaaz, M., Khan, P., Hussain, A., ... & Imtaiyaz Hassan, M. (2021). Targeting cyclin‐dependent kinase 6 by vanillin inhibits proliferation of breast and lung cancer cells: Combined computational and biochemical studies. Journal of Cellular Biochemistry.

Nusantoro, Y. R., & Fadlan, A. (2021). In Silico Studies on Isatinyl-2-aminobenzoylhydrazone Transition Metal Complexes against Cyclin-dependent Kinase 6 (CDK6). Pharmacy Reports, epr1-epr1.

Yousuf, M., Shamsi, A., Khan, P., Shahbaaz, M., AlAjmi, M. F., Hussain, A., ... & Hassan, M. (2020). Ellagic acid controls cell proliferation and induces apoptosis in breast cancer cells via inhibition of cyclin-dependent kinase 6. International journal of molecular sciences, 21(10), 3526.

Duan, L., Yan, Y., Wang, G., Xing, Y. L., Sun, J., & Wang, L. L. (2020). ΜiR-182-5p functions as a tumor suppressor to sensitize human ovarian cancer cells to cisplatin through direct targeting the cyclin dependent kinase 6 (CDK6). J. BUON, 25, 2279-2286.

Li, F., Jiang, Z., Shao, X., & Zou, N. (2020). Downregulation of lncRNA NR2F2 antisense RNA 1 induces G1 arrest of colorectal cancer cells by downregulating cyclin-dependent kinase 6. Digestive diseases and sciences, 65(2), 464-469.

Jin, J., Chen, X., Chen, J., & Geng, X. (2020). Long noncoding RNA MACC1‑AS1 is a potential sponge of microRNA‑34a in cervical squamous cell carcinoma and upregulates cyclin‑dependent kinase 6. Oncology letters, 19(3), 2339-2345.

Zhang, J., Zhang, L., Xu, Y., Jiang, S., & Shao, Y. (2018). Deciphering the binding behavior of flavonoids to the cyclin dependent kinase 6/cyclin D complex. PloS one, 13(5), e0196651.

Wu, H., Tao, J., Li, X., Zhang, T., Zhao, L., Wang, Y., ... & Song, G. (2017). Microrna‐206 prevents the pathogenesis of hepatocellular carcinoma by modulating expression of met proto‐oncogene and cyclin‐dependent kinase 6 in mice. Hepatology, 66(6), 1952-1967.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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