Mouse Anti-CDKAL1 Recombinant Antibody (EG617) (CBMAB-EN744-LY)

Name: Mouse Anti-CDKAL1 Recombinant Antibody (EG617)
SKU: CBMAB-EN744-LY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CDKAL1 Recombinant Antibody (EG617) (CBMAB-EN744-LY) in IF

Immunofluorescent analysis using anti-CDKAL1 and anti-CD133 antibodies in RD (left) and RH30 (right) cells. Scale bars, 50 μm. ...View More

Huang, R., Yamamoto, T., Nakata, E., Ozaki, T., Kurozumi, K., Wei, F., ... & Fujimura, A. (2023). CDKAL1 Drives the Maintenance of Cancer Stem-Like Cells by Assembling the eIF4F Translation Initiation Complex. Advanced Science, 10(12), 2206542.

Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CDKAL1 Recombinant Antibody (EG617) (CBMAB-EN744-LY) in WB

Transformed C2C12 cells showed elevated expression levels of CDKAL1. ...View More

Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CDKAL1 Recombinant Antibody (EG617) (CBMAB-EN744-LY) in IF

Immunofluorescent analysis of normal and pathological specimens using anti-CDKAL1 and anti-SALL2 antibodies. Note that CDKAL1high tumor cells exhibit SALL2high properties in RMS. Scale bars, 100 μm. ...View More

Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CDKAL1 Recombinant Antibody (EG617) (CBMAB-EN744-LY) in WB

Insulin B protein levels in control LF and GFP esiRNA-treated cells stimulated and not stimulated to produce insulin and in cdkal1 esiRNA (70 nM) knockdown cells. C. Mouse antibodies were used in Western blots against Insulin B and Cdkal1 or GAPDH. Insulin production was induced by low and high glucose, Tolbutamide, Theophyllin and KCl. Protein levels in cell lysates in Western blots were quantitated with Image J software and normalized to GAPDH levels and expressed as fold-change from control unstimulated (SF) cell lysates. LF - Lipofectamine (1ul); Control GFP-esiRNA 70 nM; cdkal1-esiRNA 70 nM. Protein (250 μg) was loaded on SDS-PAGE gels and immune blotted using mouse antibodies against Insulin B, Cdkal1, or GAPDH. GAPDH was used as a loading control. ...View More

Narendran, A., Vangaveti, S., Ranganathan, S. V., Eruysal, E., Craft, M., Alrifai, O., ... & Agris, P. F. (2021). Silencing of the tRNA Modification Enzyme Cdkal1 Effects Functional Insulin Synthesis in NIT-1 Cells: tRNALys3 Lacking ms2-(ms2t6A37) is Unable to Establish Sufficient Anticodon: Codon Interactions to Decode the Wobble Codon AAG. Frontiers in molecular biosciences, 7, 584228.

Distributed under Open Access license CC BY 4.0, without modification.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

EG617

Application

ELISA, WB, IP, IF

Immunogen

Amino acids 1-300 mapping at the N-terminus of CDKAL1 of human.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, λ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

CDK5 Regulatory Subunit Associated Protein 1 Like 1

Entrez Gene ID

Human	54901
Rat	361243

UniProt ID

Human	Q5VV42
Rat	K9N0M9

Function

Catalyzes the methylthiolation of N6-threonylcarbamoyladenosine (t6A), leading to the formation of 2-methylthio-N6-threonylcarbamoyladenosine (ms2t6A) at position 37 in tRNAs that read codons beginning with adenine.

Biological Process

Maintenance of translational fidelity Source: Ensembl
tRNA methylthiolation Source: GO_Central
tRNA modification Source: Reactome

Cellular Location

Endoplasmic reticulum membrane. Is a tail-anchored protein that exploits the TCR40 pathway for insertion into the endoplasmic reticulum.

Involvement in disease

Diabetes mellitus, non-insulin-dependent (NIDDM): A multifactorial disorder of glucose homeostasis caused by a lack of sensitivity to the body's own insulin. Affected individuals usually have an obese body habitus and manifestations of a metabolic syndrome characterized by diabetes, insulin resistance, hypertension and hypertriglyceridemia. The disease results in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.

Topology

Helical: 556-578

Yu, X. Y., Song, L. P., Wei, S. D., Wen, X. L., & Liu, D. B. (2021). CDK5 Regulatory Subunit-Associated Protein 1-Like 1 Gene Polymorphisms and Gestational Diabetes Mellitus Risk: A Trial Sequential Meta-Analysis of 13,306 Subjects. Frontiers in Endocrinology, 12.

Take, K., Waki, H., Sun, W., Wada, T., Yu, J., Nakamura, M., ... & Kadowaki, T. (2017). CDK5 regulatory subunit-associated protein 1-like 1 negatively regulates adipocyte differentiation through activation of Wnt signaling pathway. Scientific reports, 7(1), 1-11.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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