Mouse Anti-CDS1 Recombinant Antibody (2D10) (CBMAB-C0859-LY)

This product is antibody recognizes CDS1. The antibody 2D10 immunoassay techniques such as: ELISA, IF, WB.
See all CDS1 antibodies

Published Data

Fig.1 Western blot of cell lysates (50 μg protein) obtained from proliferating cells (10% FCS) and differentiated cells in either 1% FCS or 1% FCS with 1 μM retinoic acid (RA).

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

2D10

Antibody Isotype

IgG1, κ

Application

ELISA, IF, WB

Basic Information

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

CDP-diacylglycerol synthase (phosphatidate cytidylyltransferase) 1

Introduction

Breakdown products of phosphoinositides are ubiquitous second messengers that function downstream of many G protein-coupled receptors and tyrosine kinases regulating cell growth, calcium metabolism, and protein kinase C activity. This gene encodes an enzyme which regulates the amount of phosphatidylinositol available for signaling by catalyzing the conversion of phosphatidic acid to CDP-diacylglycerol. This enzyme is an integral membrane protein localized to two subcellular domains, the matrix side of the inner mitochondrial membrane where it is thought to be involved in the synthesis of phosphatidylglycerol and cardiolipin and the cytoplasmic side of the endoplasmic reticulum where it functions in phosphatidylinositol biosynthesis. Two genes encoding this enzyme have been identified in humans, one mapping to human chromosome 4q21 and a second to 20p13. [provided by RefSeq, Jul 2008]

Entrez Gene ID

1040

UniProt ID

Q92903

Alternative Names

CDP-Diacylglycerol Synthase 1; CTP:Phosphatidate Cytidylyltransferase 1; CDP-Diglyceride Pyrophosphorylase 1; CDP-Diglyceride Synthase 1; CDP-DAG Synthase 1; CDP-DG Synthase 1; EC 2.7.7.41; CDS 1;

Function

Catalyzes the conversion of phosphatidic acid (PA) to CDP-diacylglycerol (CDP-DAG), an essential intermediate in the synthesis of phosphatidylglycerol, cardiolipin and phosphatidylinositol (PubMed:9407135, PubMed:25375833).
Exhibits almost no acyl chain preference for PA, showing no discrimination for the sn-1/sn-2 acyl chain composition of PAs (PubMed:25375833).
Plays an important role in regulating the growth of lipid droplets which are storage organelles at the center of lipid and energy homeostasis (PubMed:26946540, PubMed:31548309).
Positively regulates the differentiation and development of adipocytes (By similarity).

Biological Process

CDP-diacylglycerol biosynthetic process Source: UniProtKB
Lipid droplet formation Source: UniProtKB
Phosphatidylinositol biosynthetic process Source: UniProtKB
Phototransduction Source: UniProtKB
Positive regulation of fat cell differentiation Source: UniProtKB
Signal transduction Source: UniProtKB

Cellular Location

Endoplasmic reticulum membrane

References

Sekiya, Y., Sawasato, K., & Nishiyama, K. I. (2021). Expression of Cds4/5 of Arabidopsis chloroplasts in E. coli reveals the membrane topology of the C‐terminal region of CDP‐diacylglycerol synthases. Genes to Cells, 26(9), 727-738.

Blunsom, N. J., & Cockcroft, S. (2020). CDP-diacylglycerol synthases (CDS): gateway to phosphatidylinositol and cardiolipin synthesis. Frontiers in cell and developmental biology, 8, 63.

Blunsom, N. J. (2020). Different CDP-diacylglycerol Synthase (CDS) enzymes participate in the synthesis of cardiolipin in mitochondria and phosphatidylinositol at the endoplasmic reticulum (Doctoral dissertation, UCL (University College London)).

Blunsom, N. J., Gomez-Espinosa, E., Ashlin, T. G., & Cockcroft, S. (2019). Sustained phospholipase C stimulation of H9c2 cardiomyoblasts by vasopressin induces an increase in CDP-diacylglycerol synthase 1 (CDS1) through protein kinase C and cFos. Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids, 1864(7), 1072-1082.

Blunsom, N. J., Gomez-Espinosa, E., Ashlin, T. G., & Cockcroft, S. (2018). Mitochondrial CDP-diacylglycerol synthase activity is due to the peripheral protein, TAMM41 and not due to the integral membrane protein, CDP-diacylglycerol synthase 1. Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids, 1863(3), 284-298.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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