Mouse Anti-CDT1 Recombinant Antibody (CP0272) (CBMAB-CP0272-LY)

Name: Mouse Anti-CDT1 Recombinant Antibody (CP0272)
SKU: CBMAB-CP0272-LY
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Published Data

Mouse Anti-CDT1 Recombinant Antibody (CP0272) (CBMAB-CP0272-LY) in WB

Western analysis (upper) and quantitation (lower) of CDT1 in nuclei of SKI-overexpressing (0 or 340ng·mL−1 plasmid) and SKI-knockdown (0 or 10nm siRNA) OZ cells (n=3). ...View More

Kawamura, E., Matsubara, T., Daikoku, A., Deguchi, S., Kinoshita, M., Yuasa, H., ... & Kawada, N. (2022). Suppression of intrahepatic cholangiocarcinoma cell growth by SKI via upregulation of the CDK inhibitor p21. FEBS Open bio, 12(12), 2122-2135.

Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CDT1 Recombinant Antibody (CP0272) (CBMAB-CP0272-LY) in WB

A673 cells were subjected to a dTB and released into S-phase in the presence of either 0.1% DMSO or 300 nM TAK-931 for the indicated timepoints and protein was collected. ...View More

Martin, J. C., Sims, J. R., Gupta, A., Hagoel, T. J., Gao, L., Lynch, M. L., ... & Ohm, J. E. (2022). CDC7 kinase (DDK) inhibition disrupts DNA replication leading to mitotic catastrophe in Ewing sarcoma. Cell Death Discovery, 8(1), 85.

Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CDT1 Recombinant Antibody (CP0272) (CBMAB-CP0272-LY) in WB

BPH1 and DU145 EV or SPOP F133V cells are treated with or without 2 mM HU for 4 h and harvested for WB with indicated antibodies. ...View More

Ma, J., Shi, Q., Cui, G., Sheng, H., Botuyan, M. V., Zhou, Y., ... & Huang, H. (2021). SPOP mutation induces replication over-firing by impairing Geminin ubiquitination and triggers replication catastrophe upon ATR inhibition. Nature communications, 12(1), 5779.

Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CDT1 Recombinant Antibody (CP0272) (CBMAB-CP0272-LY) in WB

Western blotting of siRNA-treated cells for Ddb1, Cdt1, cyclin E1, cyclin E2 and GAPDH. ...View More

Lee, C., Fernandez, K. J., Alexandrou, S., Sergio, C. M., Deng, N., Rogers, S., ... & Caldon, C. E. (2020). Cyclin E2 promotes whole genome doubling in breast cancer. Cancers, 12(8), 2268.

Distributed under Open Access license CC BY 4.0, without modification.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CP0272

Application

ELISA, WB, IF, IP, IHC-P

Immunogen

Amino acids 247-546 mapping at the C-terminus of Cdt1 of human.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Chromatin Licensing And DNA Replication Factor 1

Introduction

The protein encoded by this gene is involved in the formation of the pre-replication complex that is necessary for DNA replication. The encoded protein can bind geminin, which prevents replication and may function to prevent this protein from initiating replication at inappropriate origins. Phosphorylation of this protein by cyclin A-dependent kinases results in degradation of the protein. [provided by RefSeq, Mar 2011]

Entrez Gene ID

Human	81620
Monkey	711530

UniProt ID

Human	Q9H211
Monkey	H2QBQ6

Alternative Names

Chromatin Licensing And DNA Replication Factor 1; DUP; Double Parked; Drosophila; Homolog Of; DNA Replication Factor Cdt1; Double Parked Homolog; RIS2;

Function

Required for both DNA replication and mitosis (PubMed:11125146, PubMed:22581055, PubMed:21856198, PubMed:14993212, PubMed:26842564).
DNA replication licensing factor, required for pre-replication complex assembly. Cooperates with CDC6 and the origin recognition complex (ORC) during G1 phase of the cell cycle to promote the loading of the mini-chromosome maintenance (MCM) complex onto DNA to generate pre-replication complexes (pre-RC)(PubMed:14672932).
Required also for mitosis by promoting stable kinetochore-microtubule attachments (PubMed:22581055).
Potential oncogene (By similarity).

Biological Process

Attachment of mitotic spindle microtubules to kinetochore Source: UniProtKB
Cell division Source: CAFA
Chromosome segregation Source: UniProtKB
Deactivation of mitotic spindle assembly checkpoint Source: CAFA
DNA replication checkpoint Source: UniProtKB
DNA replication initiation Source: Reactome
DNA replication preinitiation complex assembly Source: CAFA
Kinetochore organization Source: CAFA
Mitotic cell cycle Source: UniProtKB
Negative regulation of protein localization to kinetochore Source: CAFA
Positive regulation of chromatin binding Source: CAFA
Positive regulation of DNA-dependent DNA replication Source: CAFA
Positive regulation of DNA replication Source: UniProtKB
Positive regulation of protein-containing complex assembly Source: CAFA
Positive regulation of protein localization to kinetochore Source: CAFA
Pre-replicative complex assembly Source: Reactome
Regulation of chromosome organization Source: CAFA
Regulation of DNA-dependent DNA replication initiation Source: UniProtKB
Regulation of DNA replication origin binding Source: CAFA
Regulation of nuclear cell cycle DNA replication Source: Ensembl
Regulation of transcription involved in G1/S transition of mitotic cell cycle Source: Reactome
Response to sorbitol Source: CAFA

Cellular Location

Nucleus; Kinetochore. Transiently localizes to kinetochores during prometaphase and metaphase.

Involvement in disease

Meier-Gorlin syndrome 4 (MGORS4): A syndrome characterized by bilateral microtia, aplasia/hypoplasia of the patellae, and severe intrauterine and postnatal growth retardation with short stature and poor weight gain. Additional clinical findings include anomalies of cranial sutures, microcephaly, apparently low-set and simple ears, microstomia, full lips, highly arched or cleft palate, micrognathia, genitourinary tract anomalies, and various skeletal anomalies. While almost all cases have primordial dwarfism with substantial prenatal and postnatal growth retardation, not all cases have microcephaly, and microtia and absent/hypoplastic patella are absent in some. Despite the presence of microcephaly, intellect is usually normal.

PTM

Two independent E3 ubiquitin ligase complexes, SCF(SKP2) and the DCX(DTL) complex, mediated CDT1 degradation in S phase. Ubiquitinated by the DCX(DTL) complex, in response to DNA damage, leading to its degradation. Ubiquitination by the DCX(DTL) complex is necessary to ensure proper cell cycle regulation and is PCNA-dependent: interacts with PCNA via its PIP-box, while the presence of the containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to its degradation. Phosphorylation at Thr-29 by CDK2 targets CDT1 for ubiquitination by SCF(SKP2) E3 ubiquitin ligase and subsequent degradation (PubMed:14993212). The interaction with GMNN protects it against ubiquitination. Deubiquitinated by USP37 (PubMed:27296872).
Phosphorylation by cyclin A-dependent kinases at Thr-29 targets CDT1 for ubiquitynation by SCF(SKP2) E3 ubiquitin ligase and subsequent degradation (PubMed:14993212). Phosphorylated at Thr-29 by MAPK8/JNK1, which blocks replication licensing in response to stress (PubMed:21856198). Binding to GMNN is not affected by phosphorylation.

Zhang, H. (2021). Regulation of DNA Replication Licensing and Re-Replication by Cdt1. International Journal of Molecular Sciences, 22(10), 5195.

Kanellou, A., Giakoumakis, N. N., Panagopoulos, A., Tsaniras, S. C., & Lygerou, Z. (2020). The licensing factor Cdt1 links cell cycle progression to the DNA damage response. Anticancer research, 40(5), 2449-2456.

Siril, Y. J., Kouketsu, A., Oikawa, M., Takahashi, T., & Kumamoto, H. (2019). Immunohistochemical assessment of chromatin licensing and DNA replication factor 1, geminin, and γ‐H2A. X in oral epithelial precursor lesions and squamous cell carcinoma. Journal of Oral Pathology & Medicine, 48(10), 888-896.

Mughal, M. J., Mahadevappa, R., & Kwok, H. F. (2019, October). DNA replication licensing proteins: Saints and sinners in cancer. In Seminars in cancer biology (Vol. 58, pp. 11-21). Academic Press.

Qian, J., Chen, Y., Xu, Y., Zhang, X., Kang, Z., Jiao, J., & Zhao, J. (2019). Interactional similarities and differences in the protein complex of PCNA and DNA replication factor C between rice and Arabidopsis. BMC plant biology, 19(1), 1-15.

Leng, F., Saxena, L., Hoang, N., Zhang, C., Lee, L., Li, W., ... & Zhang, H. (2018). Proliferating cell nuclear antigen interacts with the CRL4 ubiquitin ligase subunit CDT2 in DNA synthesis–induced degradation of CDT1. Journal of Biological Chemistry, 293(49), 18879-18889.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

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