Mouse Anti-CELSR1 Recombinant Antibody (CBWJC-2474) (CBMAB-C3543WJ)

Name: Mouse Anti-CELSR1 Recombinant Antibody (CBWJC-2474)
SKU: CBMAB-C3543WJ
Rating: 5 (1 reviews)

See all CELSR1 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBWJC-2474

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 2684-2809 mapping near the C-terminus of CELSR1 of human.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Cadherin EGF LAG Seven-Pass G-Type Receptor 1

Introduction

The protein encoded by this gene is a member of the flamingo subfamily, part of the cadherin superfamily. The flamingo subfamily consists of nonclassic-type cadherins; a subpopulation that does not interact with catenins. The flamingo cadherins are located at the plasma membrane and have nine cadherin domains, seven epidermal growth factor-like repeats and two laminin A G-type repeats in their ectodomain. They also have seven transmembrane domains, a characteristic unique to this subfamily. It is postulated that these proteins are receptors involved in contact-mediated communication, with cadherin domains acting as homophilic binding regions and the EGF-like domains involved in cell adhesion and receptor-ligand interactions. This particular member is a developmentally regulated, neural-specific gene which plays an unspecified role in early embryogenesis. [provided by RefSeq, Jul 2008]

Entrez Gene ID

9620

UniProt ID

Q9NYQ6

Alternative Names

Cadherin EGF LAG Seven-Pass G-Type Receptor 1; Adhesion G Protein-Coupled Receptor C1; Cadherin Family Member 9; Flamingo Homolog 2; HFMI2; CDHF9; FMI2; Cadherin, EGF LAG Seven-Pass G-Type Receptor 1, Flamingo (Drosophila) Homolog;

Function

Receptor that may have an important role in cell/cell signaling during nervous system formation.

Biological Process

Apical protein localization Source: UniProtKB
Cell-cell adhesion Source: GO_Central
Central nervous system development Source: UniProtKB
Establishment of body hair planar orientation Source: UniProtKB
Establishment of planar polarity Source: UniProtKB
Establishment of planar polarity of embryonic epithelium Source: UniProtKB
Homophilic cell adhesion via plasma membrane adhesion molecules Source: InterPro
Lateral sprouting involved in lung morphogenesis Source: UniProtKB
Neural tube closure Source: UniProtKB
Neuron migration Source: UniProtKB
Orthogonal dichotomous subdivision of terminal units involved in lung branching morphogenesis Source: UniProtKB
Planar dichotomous subdivision of terminal units involved in lung branching morphogenesis Source: UniProtKB
Protein localization involved in establishment of planar polarity Source: UniProtKB
Regulation of actin cytoskeleton organization Source: UniProtKB
Rho protein signal transduction Source: UniProtKB
Wnt signaling pathway, planar cell polarity pathway Source: ParkinsonsUK-UCL

Cellular Location

Cell membrane

Involvement in disease

Neural tube defects (NTD): Congenital malformations of the central nervous system and adjacent structures related to defective neural tube closure during the first trimester of pregnancy. Failure of neural tube closure can occur at any level of the embryonic axis. Common NTD forms include anencephaly, myelomeningocele and spina bifida, which result from the failure of fusion in the cranial and spinal region of the neural tube. NTDs have a multifactorial etiology encompassing both genetic and environmental components.

Topology

Extracellular: 22-2469
Helical: 2470-2490
Cytoplasmic: 2491-2501
Helical: 2502-2522
Extracellular: 2523-2527
Helical: 2528-2548
Cytoplasmic: 2549-2572
Helical: 2573-2593
Extracellular: 2594-2611
Helical: 2612-2632
Cytoplasmic: 2633-2655
Helical: 2656-2676
Extracellular: 2677-2683
Helical: 2684-2704
Cytoplasmic: 2705-3014

PTM

The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.

Usami, F. M., Arata, M., Shi, D., Oka, S., Higuchi, Y., Tissir, F., ... & Fujimori, T. (2021). Intercellular and intracellular cilia orientation is coordinated by CELSR1 and CAMSAP3 in oviduct multi-ciliated cells. Journal of Cell Science, 134(4), jcs257006.

Wang, L. H., Zhang, G. L., Liu, X. Y., Peng, A., Ren, H. Y., Huang, S. H., ... & Wang, X. J. (2020). CELSR1 promotes neuroprotection in cerebral ischemic injury mainly through the Wnt/PKC signaling pathway. International journal of molecular sciences, 21(4), 1267.

Goffinet, A. M., & Tissir, F. (2017, September). Seven pass cadherins CELSR1-3. In Seminars in cell & developmental biology (Vol. 69, pp. 102-110). Academic Press.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-CELSR1 Recombinant Antibody (CBWJC-2474)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry