Mouse Anti-CEMIP Recombinant Antibody (K0001) (CBMAB-K0001-LY)

Name: Mouse Anti-CEMIP Recombinant Antibody (K0001)
SKU: CBMAB-K0001-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

K0001

Application

WB, ELISA, IHC

Immunogen

VTLDTEDHKA peptide.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
ELISA	1:100-1:2,000
IHC	1:10-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

0.02% sodium azide

Concentration

1 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Cell Migration Inducing Hyaluronan Binding Protein

Introduction

CEMIP (Cell Migration Inducing Hyaluronan Binding Protein) is a Protein Coding gene. Diseases associated with CEMIP include Nonsyndromic Deafness. Among its related pathways are Glycosaminoglycan metabolism and Metabolism. Gene Ontology (GO) annotations related to this gene include hyaluronic acid binding and clathrin heavy chain binding. An important paralog of this gene is TMEM2.

Entrez Gene ID

57214

UniProt ID

Q8WUJ3

Alternative Names

Cell Migration Inducing Hyaluronan Binding Protein; KIAA1199; Hyaluronan-Binding Protein Involved In Hyaluronan Depolymerization; Cell Migration Inducing Protein; Hyaluronan Binding; Cell Migration-Inducing And Hyaluronan-Binding Protein; Colon Cancer Secreted Protein 1;

Function

Mediates depolymerization of hyaluronic acid (HA) via the cell membrane-associated clathrin-coated pit endocytic pathway. Binds to hyaluronic acid. Hydrolyzes high molecular weight hyaluronic acid to produce an intermediate-sized product, a process that may occur through rapid vesicle endocytosis and recycling without intracytoplasmic accumulation or digestion in lysosomes. Involved in hyaluronan catabolism in the dermis of the skin and arthritic synovium. Positively regulates epithelial-mesenchymal transition (EMT), and hence tumor cell growth, invasion and cancer dissemination. In collaboration with HSPA5/BIP, promotes cancer cell migration in a calcium and PKC-dependent manner. May be involved in hearing.

Biological Process

Hyaluronan biosynthetic process Source: Reactome
Hyaluronan catabolic process Source: UniProtKB
Positive regulation of cell migration Source: UniProtKB
Positive regulation of peptidyl-threonine phosphorylation Source: UniProtKB
Positive regulation of protein kinase C activity Source: UniProtKB
Positive regulation of protein targeting to membrane Source: UniProtKB
Positive regulation of release of sequestered calcium ion into cytosol Source: UniProtKB
Sensory perception of sound Source: UniProtKB

Cellular Location

Secreted; Nucleus; Cytoplasm; Cell membrane; Endoplasmic reticulum; Clathrin-coated pit. Retained in the endoplasmic reticulum (ER) in a HSPA5/BIP-dependent manner. Colocalized with clathrin heavy chain/CLTC in clathrin-coated vesicles. Strongly detected in the cytoplasm of breast carcinoma cells, whereas poorly detected in adjacent normal epithelial cells, stromal cells, or benign breast tissues. Localized in the nucleus and cytoplasm of colon adenocarcinomas.

PTM

N-glycosylated; glycosylation is not necessary for HA-binding.

Chen, Y., Zhou, H., Jiang, W. J., Wang, J. F., Tian, Y., Jiang, Y., & Xia, B. R. (2022). The role of CEMIP in tumors: An update based on cellular and molecular insights. Biomedicine & Pharmacotherapy, 146, 112504.

Chen, Y., Li, L., & Zhang, J. (2021). Cell migration inducing hyaluronidase 1 (CEMIP) activates STAT3 pathway to facilitate cell proliferation and migration in breast cancer. Journal of Receptors and Signal Transduction, 41(2), 145-152.

Song, M., Liu, J., Zheng, X., Zhou, X., Feng, Z., & Hu, W. (2021). MiR-148a-3p targets CEMIP to suppress the genesis of gastric cancer cells. Biochemical and Biophysical Research Communications, 575, 42-49.

Sato, S., Mizutani, Y., Yoshino, Y., Masuda, M., Miyazaki, M., Hara, H., & Inoue, S. (2021). Pro-inflammatory cytokines suppress HYBID (hyaluronan (HA)-binding protein involved in HA depolymerization/KIAA1199/CEMIP)-mediated HA metabolism in human skin fibroblasts. Biochemical and Biophysical Research Communications, 539, 77-82.

Ohtsuki, T., Hatipoglu, O. F., Asano, K., Inagaki, J., Nishida, K., & Hirohata, S. (2020). Induction of CEMIP in chondrocytes by inflammatory cytokines: underlying mechanisms and potential involvement in osteoarthritis. International journal of molecular sciences, 21(9), 3140.

Dokoshi, T., Zhang, L. J., Li, F., Nakatsuji, T., Butcher, A., Yoshida, H., ... & Gallo, R. L. (2020). Hyaluronan degradation by Cemip regulates host defense against staphylococcus aureus skin infection. Cell reports, 30(1), 61-68.

Hu, R., & Lu, Z. (2020). Long non‑coding RNA HCP5 promotes prostate cancer cell proliferation by acting as the sponge of miR‑4656 to modulate CEMIP expression. Oncology reports, 43(1), 328-336.

Li, L., Pan, Y., Mo, X., Wei, T., Song, J., Luo, M., ... & Yang, J. (2020). A novel metastatic promoter CEMIP and its downstream molecular targets and signaling pathway of cellular migration and invasion in SCLC cells based on proteome analysis. Journal of Cancer Research and Clinical Oncology, 146(10), 2519-2534.

Liang, G., Fang, X., Yang, Y., & Song, Y. (2018). Knockdown of CEMIP suppresses proliferation and induces apoptosis in colorectal cancer cells: downregulation of GRP78 and attenuation of unfolded protein response. Biochemistry and Cell Biology, 96(3), 332-341.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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