Mouse Anti-CENPF Recombinant Antibody (14C10/1D8) (CBMAB-C0335-LY)

Name: Mouse Anti-CENPF Recombinant Antibody (14C10/1D8)
SKU: CBMAB-C0335-LY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CENPF Recombinant Antibody (14C10/1D8) (CBMAB-C0335-LY) in IF and FC

MPP1 expression is increased in G2/M cells. The upper panels show confocal images of indirect immunofluorescence of HeLa cells stained with the anti-MPP1 (red) and anti-mitosin (green) antibodies. Lower panels, FACS analysis of HeLa cells stained with the same antibodies together with Hoechst and processed as described under "Experimental Procedures." ...View More

Abaza, A., Soleilhac, J. M., Westendorf, J., Piel, M., Crevel, I., Roux, A., & Pirollet, F. (2003). M phase phosphoprotein 1 is a human plus-end-directed kinesin-related protein required for cytokinesis. Journal of Biological Chemistry, 278(30), 27844-27852.

Distributed under Open Access license CC BY 4.0, without modification.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

14C10/1D8

Application

WB, IP, IHC-P, FC, ICC/IF

Immunogen

Protein fragment expressed in E. coli corresponding to amino acids 1759-2093.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

1 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Centromere Protein F

Introduction

This gene encodes a protein that associates with the centromere-kinetochore complex. The protein is a component of the nuclear matrix during the G2 phase of interphase. In late G2 the protein associates with the kinetochore and maintains this association through early anaphase. It localizes to the spindle midzone and the intracellular bridge in late anaphase and telophase, respectively, and is thought to be subsequently degraded. The localization of this protein suggests that it may play a role in chromosome segregation during mitotis. It is thought to form either a homodimer or heterodimer. Autoantibodies against this protein have been found in patients with cancer or graft versus host disease. [provided by RefSeq, Jul 2008]

Entrez Gene ID

1063

UniProt ID

P49454

Alternative Names

Centromere Protein F; Mitosin; Centromere Protein F, 350/400kDa; Kinetochore Protein CENPF; AH Antigen; Centromere Protein F, 350/400kDa (Mitosin); Cell-Cycle-Dependent 350K Nuclear Protein; CENP-F Kinetochore Protein;

Function

Required for kinetochore function and chromosome segregation in mitosis. Required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1. Regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation. May play a regulatory or permissive role in the normal embryonic cardiomyocyte cell cycle and in promoting continued mitosis in transformed, abnormally dividing neonatal cardiomyocytes. Interaction with RB directs embryonic stem cells toward a cardiac lineage. Involved in the regulation of DNA synthesis and hence cell cycle progression, via its C-terminus. Has a potential role regulating skeletal myogenesis and in cell differentiation in embryogenesis. Involved in dendritic cell regulation of T-cell immunity against chlamydia.

Biological Process

Cell differentiation Source: UniProtKB-KW
Cell division Source: UniProtKB-KW
Chromosome segregation Source: UniProtKB
DNA biosynthetic process Source: UniProtKB-KW
Kidney development Source: GO_Central
Kinetochore assembly Source: UniProtKB
Metaphase plate congression Source: UniProtKB
Mitotic cell cycle Source: UniProtKB
Mitotic spindle assembly checkpoint Source: UniProtKB
Mitotic spindle organization Source: Reactome
Muscle organ development Source: UniProtKB-KW
Negative regulation of transcription, DNA-templated Source: UniProtKB
Protein transport Source: UniProtKB
Regulation of cell cycle Source: Reactome
Regulation of G2/M transition of mitotic cell cycle Source: UniProtKB
Regulation of striated muscle tissue development Source: UniProtKB
Response to drug Source: UniProtKB
Ventricular system development Source: GO_Central

Cellular Location

Spindle; Nucleus matrix; Perinuclear region; Kinetochore. Relocalizes to the kinetochore/centromere (coronal surface of the outer plate) and the spindle during mitosis. Observed in nucleus during interphase but not in the nucleolus. At metaphase becomes localized to areas including kinetochore and mitotic apparatus as well as cytoplasm. By telophase, is concentrated within the intracellular bridge at either side of the mid-body.

Involvement in disease

Stromme syndrome (STROMS): An autosomal recessive congenital disorder characterized by intestinal atresia, ocular anomalies, microcephaly, and renal and cardiac abnormalities in some patients. The disease has features of a ciliopathy, and lethality in early childhood is observed in severe cases.

PTM

Hyperphosphorylated during mitosis.

Huang, Y., Chen, X., Wang, L., Wang, T., Tang, X., & Su, X. (2021). Centromere Protein F (CENPF) Serves as a Potential Prognostic Biomarker and Target for Human Hepatocellular Carcinoma. Journal of Cancer, 12(10), 2933.

Chen, H., Wu, F., Xu, H., Wei, G., Ding, M., Xu, F., ... & Xia, H. (2021). Centromere protein F promotes progression of hepatocellular carcinoma through ERK and cell cycle-associated pathways. Cancer gene therapy, 1-10.

Chen, H., Wang, X., Wu, F., Mo, X., Hu, C., Wang, M., ... & Lan, L. (2021). Centromere protein F is identified as a novel therapeutic target by genomics profile and contributing to the progression of pancreatic cancer. Genomics, 113(1), 1087-1095.

Li, R., Wang, X., Zhao, X., Zhang, X., Chen, H., Ma, Y., & Liu, Y. (2020). Centromere protein F and Forkhead box M1 correlation with prognosis of non‑small cell lung cancer. Oncology letters, 19(2), 1368-1374.

Lourido, L., Ruiz-Romero, C., Picchi, F., Diz-Rosales, N., Vilaboa-Galán, S., Fernández-López, C., ... & Blanco, F. J. (2020, October). Association of serum anti-centromere protein F antibodies with clinical response to infliximab in patients with rheumatoid arthritis: A prospective study. In Seminars in Arthritis and Rheumatism (Vol. 50, No. 5, pp. 1101-1108). WB Saunders.

Yang, X., Miao, B. S., Wei, C. Y., Dong, R. Z., Gao, P. T., Zhang, X. Y., ... & Cai, J. B. (2019). Lymphoid‐specific helicase promotes the growth and invasion of hepatocellular carcinoma by transcriptional regulation of centromere protein F expression. Cancer science, 110(7), 2133-2144.

Hur, K., Jearn, L. H., & Kim, T. Y. (2019). Centromere protein-F-like pattern in a patient with rheumatoid arthritis. Annals of laboratory medicine, 39(2), 227-228.

Göbel, C., Özden, C., Schroeder, C., Hube-Magg, C., Kluth, M., Möller-Koop, C., ... & Luebke, A. M. (2018). Upregulation of centromere protein F is linked to aggressive prostate cancers. Cancer management and research, 10, 5491.

Shahid, M., Lee, M. Y., Piplani, H., Andres, A. M., Zhou, B., Yeon, A., ... & Kim, J. (2018). Centromere protein F (CENPF), a microtubule binding protein, modulates cancer metabolism by regulating pyruvate kinase M2 phosphorylation signaling. Cell Cycle, 17(24), 2802-2818.

Li, S., Li, X., Xu, A., Zhang, B., He, X., Chen, H., & Huang, J. (2018). Screening and clinical evaluation of dominant peptides of centromere protein F antigen for early diagnosis of hepatocellular carcinoma. Molecular medicine reports, 17(3), 4720-4728.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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