Mouse Anti-CENPN Recombinant Antibody (4A5-1C11) (CBMAB-C6603-LY)

Name: Mouse Anti-CENPN Recombinant Antibody (4A5-1C11)
SKU: CBMAB-C6603-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

4A5-1C11

Application

ELISA, WB

Immunogen

Full length recombinant BM039.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Centromere Protein N

Introduction

The protein encoded by this gene forms part of the nucleosome-associated complex and is important for kinetochore assembly. It is bound to kinetochores during S phase and G2 and recruits other proteins to the centromere. Pseudogenes of this gene are located on chromosome 2. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jul 2012]

Entrez Gene ID

55839

UniProt ID

Q96H22

Alternative Names

Centromere Protein N; Interphase Centromere Complex Protein 32; C16orf60; CENP-N; ICEN32; Chromosome 16 Open Reading Frame 60; BM039;

Function

Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. CENPN is the first protein to bind specifically to CENPA nucleosomes and the direct binding of CENPA nucleosomes by CENPN is required for centromere assembly. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate.

Biological Process

CENP-A containing nucleosome assembly Source: Reactome
Chromosome segregation Source: InterPro
Kinetochore assembly Source: InterPro
Mitotic spindle organization Source: Reactome

Cellular Location

Nucleus; Kinetochore. Localizes exclusively in the kinetochore domain of centromeres. Kinetochore-bound levels decrease when cells enter mitosis and increase again when cells exit mitosis.

Wang, Q., Yu, X., Zheng, Z., Chen, F., Yang, N., & Zhou, Y. (2021). Centromere protein N may be a novel malignant prognostic biomarker for hepatocellular carcinoma. PeerJ, 9, e11342.

Wu, H., Zhou, Y., Wu, H., Xu, L., Yan, Y., Tong, X., & Yan, H. (2021). CENPN Acts as a Novel Biomarker that Correlates With the Malignant Phenotypes of Glioma Cells. Frontiers in genetics, 1899.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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