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Mouse Anti-CENPS Recombinant Antibody (6F16) (CBMAB-A0406-LY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
6F16
Antibody Isotype
IgG2b, κ
Application
WB, ELISA

Basic Information

Immunogen
APITD1 full-length recombinant protein.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
None
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Centromere Protein S
Introduction
This gene was identified in the neuroblastoma tumour suppressor candidate region on chromosome 1p36. It contains a TFIID-31 domain, similar to that found in TATA box-binding protein-associated factor, TAF(II)31, which is required for p53-mediated transcription activation. This gene was expressed at very low levels in neuroblastoma tumours, and was shown to reduce cell growth in neuroblastoma cells, suggesting that it may have a role in a cell death pathway. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq]
Entrez Gene ID
378708
UniProt ID
Q8N2Z9
Alternative Names
CENP-S; CENPS; MGC32686
Function
DNA-binding component of the Fanconi anemia (FA) core complex. Required for the normal activation of the FA pathway, leading to monoubiquitination of the FANCI-FANCD2 complex in response to DNA damage, cellular resistance to DNA cross-linking drugs, and prevention of chromosomal breakage (PubMed:20347428, PubMed:20347429).
In complex with CENPX (MHF heterodimer), crucial cofactor for FANCM in both binding and ATP-dependent remodeling of DNA. Stabilizes FANCM (PubMed:20347428, PubMed:20347429).
In complex with CENPX and FANCM (but not other FANC proteins), rapidly recruited to blocked forks and promotes gene conversion at blocked replication forks (PubMed:20347428).
In complex with CENPT, CENPW and CENPX (CENP-T-W-S-X heterotetramer), involved in the formation of a functional kinetochore outer plate, which is essential for kinetochore-microtubule attachment and faithful mitotic progression (PubMed:19620631).
As a component of MHF and CENP-T-W-S-X complexes, binds DNA and bends it to form a nucleosome-like structure (PubMed:20347428, PubMed:22304917).
DNA-binding function is fulfilled in the presence of CENPX, with the following preference for DNA substates: Holliday junction> double-stranded> splay arm> single-stranded. Does not bind DNA on its own (PubMed:20347428, PubMed:20347429).
Biological Process
Cell division Source: UniProtKB-KW
Cellular response to DNA damage stimulus Source: UniProtKB
CENP-A containing nucleosome assembly Source: Reactome
DNA repair Source: UniProtKB
Interstrand cross-link repair Source: Reactome
Kinetochore assembly Source: InterPro
Mitotic spindle organization Source: Reactome
Positive regulation of protein ubiquitination Source: UniProtKB
Replication fork processing Source: UniProtKB
Resolution of meiotic recombination intermediates Source: UniProtKB
Cellular Location
Nucleus; Centromere; Kinetochore. Assembly of CENPS and CENPX and its partner subunits CENPT and CENPW at centromeres occurs through a dynamic exchange mechanism. Although exchange is continuous in the cell cycle, de novo assembly starts principally during mid-late S phase and is complete by G2. CENPS is more stably bound at the kinetochore than CENPX (PubMed:19620631, PubMed:24522885). During S phase, rapidly recruited to DNA interstrand cross-links that block replication (PubMed:20347428). Recruited to DNA damage sites about 20 minutes following UV irradiation, reaching a plateau after approximately 40 minutes (PubMed:24522885).
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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