Mouse Anti-CEP170 Recombinant Antibody (0443) (CBMAB-0443-CN)

Name: Mouse Anti-CEP170 Recombinant Antibody (0443)
SKU: CBMAB-0443-CN
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CEP170 Recombinant Antibody (0443) (CBMAB-0443-CN) in WB

Westerns blots of centrosomal fractions purified from asynchronous (AS) or from mitotic (M) HeLa cells synchronized with nocodazole; centrosomal markers: CEP170, g-tubulin; calreticulin and HSPB5: negative controls; Total: proteins levels in total cell extracts. ...View More

Fuchs, M., Luthold, C., Guilbert, S. M., Varlet, A. A., Lambert, H., Jetté, A., ... & Lavoie, J. N. (2015). A role for the chaperone complex BAG3-HSPB8 in actin dynamics, spindle orientation and proper chromosome segregation during mitosis. PLoS genetics, 11(10), e1005582.

Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CEP170 Recombinant Antibody (0443) (CBMAB-0443-CN) in WB

Western blots for pTBK1, TBK1, pPLK1, PLK1, CEP170, γ-tubulin and alpha tubulin from different fractions collected during centrosome isolation from H460 and A549 cells. pTBK1 and TBK1 are enriched in the centrosomal fraction (Fraction-4). Purity of the centrosomal fraction is indicated by high levels of γ-Tubulin in Fraction-4. CF indicates the centrosomal fraction. Lysate lane represents whole cell lysate prepared from asynchronously growing H460 or A549 cells. Experiments were repeated three times and representative images are presented. ...View More

Pillai, S., Nguyen, J., Johnson, J., Haura, E., Coppola, D., & Chellappan, S. (2015). Tank binding kinase 1 is a centrosome-associated kinase necessary for microtubule dynamics and mitosis. Nature communications, 6(1), 10072.

Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CEP170 Recombinant Antibody (0443) (CBMAB-0443-CN) in IF

Cells were stained with anti-cep170 (green) and anti-centrin-2 (red) Abs. DAPI staining (blue) was used to visualize DNA. Representative images of different siCDKL5 phenotypes are shown on the left. The percentage of cells with the indicated phenotypes is reported to the right as mean ± S.E.M (n = 2; *p < 0.05; unpaired t-test; approximately 200 cells were analyzed per condition). n.s. = not significant. Scale bar, 10 µm. ...View More

Barbiero, I., Valente, D., Chandola, C., Magi, F., Bergo, A., Monteonofrio, L., ... & Kilstrup-Nielsen, C. (2017). CDKL5 localizes at the centrosome and midbody and is required for faithful cell division. Scientific reports, 7(1), 6228.

Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CEP170 Recombinant Antibody (0443) (CBMAB-0443-CN) in ICC

Cells were co-stained with DAPI to visualize the nuclei and analyzed by confocal microscopy. ...View More

Mukhopadhyay, A., Sehgal, L., Bose, A., Gulvady, A., Senapati, P., Thorat, R., ... & Dalal, S. N. (2016). 14-3-3γ prevents centrosome amplification and neoplastic progression. Scientific reports, 6(1), 26580.

Distributed under Open Access license CC BY 4.0, without modification.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

0443

Application

FC, IF, IP, WB

Immunogen

Recombinant protein derived from the N-terminal region of the human Cep170 protein.

Host Species

Mouse

Specificity

Human, Mouse

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IF(ICC)	2 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Preservative

0.1% sodium azide

Concentration

0.5 mg/ml

Purity

>95%, as determined by SDS-PAGE analysis

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Centrosomal Protein 170

Introduction

The product of this gene is a component of the centrosome, a non-membraneous organelle that functions as the major microtubule-organizing center in animal cells. This protein plays a role in microtubule organization. It's required for centriole subdistal appendage assembly.

Entrez Gene ID

9859

UniProt ID

Q5SW79

Alternative Names

KAB; FAM68A; KIAA0470

Function

Plays a role in microtubule organization (PubMed:15616186).
Required for centriole subdistal appendage assembly (PubMed:28422092).

Cellular Location

Centrosome; Centriole; Spindle pole. Associated with the mature mother centriole. Associated with spindle microtubules during mitosis. Localizes to the distal appendage region of the centriole (PubMed:31789463). Localizes at the centriole proximal ends (PubMed:31789463).

PTM

Phosphorylated; probably by PLK1.

Qin, Y., Zhou, Y., Shen, Z., Xu, B., Chen, M., Li, Y., ... & Gao, F. (2019). WDR62 is involved in spindle assembly by interacting with CEP170 in spermatogenesis. Development, 146(20), dev174128.

Bärenz, F., Kschonsak, Y. T., Meyer, A., Jafarpour, A., Lorenz, H., & Hoffmann, I. (2018). Ccdc61 controls centrosomal localization of Cep170 and is required for spindle assembly and symmetry. Molecular biology of the cell, 29(26), 3105-3118.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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