Mouse Anti-CEP55 Recombinant Antibody (CBWJC-2498) (CBMAB-C3568WJ)

Name: Mouse Anti-CEP55 Recombinant Antibody (CBWJC-2498)
SKU: CBMAB-C3568WJ
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CEP55 Recombinant Antibody (CBWJC-2498) (CBMAB-C3568WJ) in Immunoblot

HT-LC3 U2-OS cells were transfected with the indicated ON-TARGETplus SMART Pool siRNAs for 48 h and subjected to immunoblotting using the indicated antibodies. ...View More

Takahashi, Y., He, H., Tang, Z., Hattori, T., Liu, Y., Young, M. M., ... & Wang, H. G. (2018). An autophagy assay reveals the ESCRT-III component CHMP2A as a regulator of phagophore closure. Nature communications, 9(1), 2855.

Distributed under Open Access license CC BY 4.0, without modification.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBWJC-2498

Application

WB, IP, IF, ELISA, IHC-P

Immunogen

Amino acids 163-462 mapping at the C-terminus of CEP55 of mouse.

Host Species

Mouse

Specificity

Mouse, Rat

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Centrosomal Protein 55

Introduction

CEP55 (Centrosomal Protein 55) is a Protein Coding gene. Diseases associated with CEP55 include Multinucleated Neurons, Anhydramnios, Renal Dysplasia, Cerebellar Hypoplasia, And Hydranencephaly and Meckel Syndrome, Type 1. Among its related pathways are DNA Damage and Cytoskeletal Signaling.

Entrez Gene ID

55165

UniProt ID

Q53EZ4

Alternative Names

Centrosomal Protein 55; Up-Regulated In Colon Cancer 6; Cancer/Testis Antigen 111; Centrosomal Protein 55kDa; C10orf3; URCC6;

Function

Plays a role in mitotic exit and cytokinesis (PubMed:16198290, PubMed:17853893).
Recruits PDCD6IP and TSG101 to midbody during cytokinesis. Required for successful completion of cytokinesis (PubMed:17853893).
Not required for microtubule nucleation (PubMed:16198290).
Plays a role in the development of the brain and kidney (PubMed:28264986).

Biological Process

Cranial skeletal system development Source: UniProtKB
Establishment of protein localization Source: UniProtKB
Midbody abscission Source: UniProtKB
Mitotic cytokinesis Source: MGI
Multicellular organism development Source: UniProtKB-KW
Regulation of phosphatidylinositol 3-kinase signaling Source: InterPro

Cellular Location

Centriole; Centrosome; Cytoplasm; Cleavage furrow; Midbody ring. Present at the centrosomes at interphase. A small portion is associated preferentially with the mother centriole, whereas the majority localizes to the pericentriolar material. During mitosis, loses affinity for the centrosome at the onset of prophase and diffuses throughout the cell. This dissociation from the centrosome is phosphorylation-dependent. May remain localized at the centrosome during mitosis in certain cell types. Appears at the cleavage furrow in late anaphase and in the midbody in cytokinesis.

Involvement in disease

Multinucleated neurons, anhydramnios, renal dysplasia, cerebellar hypoplasia and hydranencephaly (MARCH): An autosomal recessive, congenital disease characterized by severe hydranencephaly with multinucleated neurons, renal aplasia or dysplasia, and hypoplastic kidneys. Hydranencephaly is an anomaly leading to replacement of the cerebral hemispheres with a fluid-filled cyst. MARCH results in death in utero or in the perinatal period.

PTM

There is a hierachy of phosphorylation, where both Ser-425 and Ser-428 are phosphorylated at the onset of mitosis, prior to Ser-436. Phosphorylation at Ser-425 and Ser-428 is required for dissociation from the centrosome at the G2/M boundary. Phosphorylation at the 3 sites, Ser-425, Ser-428 and Ser-436, is required for protein function at the final stages of cell division to complete cytokinesis successfully.

Huang, R. H., Yang, W. K., Wu, C. M., Yeh, C. M., & Sung, W. W. (2021). Over-expression of CEP55 predicts favorable prognosis in colorectal cancer patients with lymph node involvement. Anticancer Research, 41(1), 543-547.

Tandon, D., & Banerjee, M. (2020). Centrosomal protein 55: A new paradigm in tumorigenesis. European Journal of Cell Biology, 99(5), 151086.

Rawlins, L. E., Jones, H., Wenger, O., Aye, M., Fasham, J., Harlalka, G. V., ... & Baple, E. L. (2019). An Amish founder variant consolidates disruption of CEP55 as a cause of hydranencephaly and renal dysplasia. European Journal of Human Genetics, 27(4), 657-662.

Yanagi, K., Sone, R., Ohga, R., & Kawahara, A. (2019). Involvement of the centrosomal protein 55 (cep55) gene in zebrafish head formation. Genes to Cells, 24(10), 642-649.

Li, F., Jin, D., Guan, L., Zhang, C. C., Wu, T., Wang, Y. J., & Gao, D. S. (2019). CEP55 promoted the migration, invasion and neuroshpere formation of the glioma cell line U251. Neuroscience letters, 705, 80-86.

Ma, X. P., Zhang, W., Wu, B. Q., & Qin, J. (2018). Correlations between mRNA levels of centrosomal protein 55 (CEP55) and clinical features of patients with lung cancer. Medical science monitor: international medical journal of experimental and clinical research, 24, 3093.

Qi, J., Liu, G., & Wang, F. (2018). High levels of centrosomal protein 55 expression is associated with poor clinical prognosis in patients with cervical cancer. Oncology letters, 15(6), 9347-9352.

Zhu, H., Chen, D., Tang, J., Huang, C., Lv, S., Wang, D., & Li, G. (2018). Overexpression of centrosomal protein 55 regulates the proliferation of glioma cell and mediates proliferation promoted by EGFRvIII in glioblastoma U251 cells. Oncology letters, 15(2), 2700-2706.

Peng, T., Zhou, W., Guo, F., Wu, H. S., Wang, C. Y., Wang, L., & Yang, Z. Y. (2017). Centrosomal protein 55 activates NF-κB signalling and promotes pancreatic cancer cells aggressiveness. Scientific reports, 7(1), 1-12.

Jiang, W., Wang, Z., & Jia, Y. (2017). CEP55 overexpression predicts poor prognosis in patients with locally advanced esophageal squamous cell carcinoma. Oncology letters, 13(1), 236-242.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

CEP55 Matched Antibody Pair (260) (CAT#: APMAB-260LY)

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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