Mouse Anti-CEP68 Recombinant Antibody (CBFYC-1726) (CBMAB-C1788-FY)

Name: Mouse Anti-CEP68 Recombinant Antibody (CBFYC-1726)
SKU: CBMAB-C1788-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYC-1726

Application

Immunogen

Recombinant human CRTAC1 Ser28-Cys661.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2b

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IF(ICC)	8-25 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

PBS, Trehalose

Preservative

None

Concentration

1 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Centrosomal Protein 68

Introduction

CEP68 (Centrosomal Protein 68) is a Protein Coding gene. Diseases associated with CEP68 include Retinitis Pigmentosa 28. Gene Ontology (GO) annotations related to this gene include protein kinase binding and protein domain specific binding. An important paralog of this gene is AKAP6.

Entrez Gene ID

23177

UniProt ID

Q76N32

Alternative Names

Centrosomal Protein 68; KIAA0582; Centrosomal Protein 68kDa; Centrosomal Protein Of 68 KDa; Cep68

Function

Involved in maintenance of centrosome cohesion, probably as part of a linker structure which prevents centrosome splitting (PubMed:18042621).
Required for localization of CDK5RAP2 to the centrosome during interphase (PubMed:24554434, PubMed:25503564).

Biological Process

Centriole-centriole cohesion Source: UniProtKB
Centrosome cycle Source: UniProtKB
Protein localization to organelle Source: UniProtKB

Cellular Location

Centrosome. Localizes to thin fibers protruding away from the proximal ends of the two centrioles. Dissociates from interphase centrosomes at the onset of mitosis.

PTM

Phosphorylation by PLK1 is required for binding to BTRC in prometaphase (PubMed:25503564). Phosphorylated directly or indirectly by NEK2 (PubMed:24554434). NEK2-mediated phosphorylation promotes CEP68 dissociation from the centrosome and its degradation at the onset of mitosis (PubMed:25704143).
Ubiquitinated and targeted for proteasomal degradation in early mitosis by the SCF(BTRC) and/or SCF(FBXW11) E3 ubiquitin-protein ligase complexes (PubMed:25704143, PubMed:25503564). Degradation is complete by prometaphase and is required for removal of CDK5RAP2 from the peripheral pericentriolar material and subsequent centriole separation (PubMed:25503564).

Perkins, J. R., Acosta-Herrera, M., Plaza-Seron, M. C., Jurado-Escobar, R., Dona, I., Garcia-Martin, E., ... & Cornejo-García, J. A. (2019). Polymorphisms in CEP68 gene associated with risk of immediate selective reactions to non-steroidal anti-inflammatory drugs. The pharmacogenomics journal, 19(2), 191-199.

Vlijm, R., Li, X., Panic, M., Rüthnick, D., Hata, S., Herrmannsdörfer, F., ... & Schiebel, E. (2018). STED nanoscopy of the centrosome linker reveals a CEP68-organized, periodic rootletin network anchored to a C-Nap1 ring at centrioles. Proceedings of the National Academy of Sciences, 115(10), E2246-E2253.

Kong, L., Yin, H., & Yuan, L. (2017). Centrosomal MCM7 strengthens the Cep68-VHL interaction and excessive MCM7 leads to centrosome splitting resulting from increase in Cep68 ubiquitination and proteasomal degradation. Biochemical and biophysical research communications, 489(4), 497-502.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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