Mouse Anti-CEP97 Recombinant Antibody (CBWJC-2503) (CBMAB-C3573WJ)

Name: Mouse Anti-CEP97 Recombinant Antibody (CBWJC-2503)
SKU: CBMAB-C3573WJ
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBWJC-2503

Antibody Isotype

IgM, κ

Application

WB, IP, IF, ELISA

Basic Information

Immunogen

Amino acids 823-842 near the C-terminus of CEP97 of human.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgM, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Centrosomal Protein 97

Introduction

CEP97 (Centrosomal Protein 97) is a Protein Coding gene. Diseases associated with CEP97 include Spinocerebellar Ataxia 11 and Townes-Brocks Syndrome. Among its related pathways are Organelle biogenesis and maintenance and Regulation of PLK1 Activity at G2/M Transition. Gene Ontology (GO) annotations related to this gene include calmodulin binding.

Entrez Gene ID

79598

UniProt ID

Q8IW35

Alternative Names

Centrosomal Protein 97; Leucine-Rich Repeat And IQ Domain-Containing Protein 2; Leucine-Rich Repeats And IQ Motif Containing 2; LRRIQ2; Centrosomal Protein Of 97 KDa; Centrosomal Protein 97kDa; 2810403B08Rik; Cep97;

Function

Acts as a key negative regulator of ciliogenesis in collaboration with CCP110 by capping the mother centriole thereby preventing cilia formation. Required for recruitment of CCP110 to the centrosome.

Biological Process

Ciliary basal body-plasma membrane docking Source: Reactome
Negative regulation of cilium assembly Source: UniProtKB
Regulation of mitotic spindle assembly Source: UniProtKB

Cellular Location

Centrosome

More Infomation

References

Lee, M., Nagashima, K., Yoon, J., Sun, J., Wang, Z., Carpenter, C., ... & Daar, I. O. (2021). CEP97 phosphorylation by Dyrk1a is critical for centriole separation during multiciliogenesis. Journal of Cell Biology, 221(1), e202102110.

Prosser, S. L., Tkach, J., Gheiratmand, L., Morrison, C. G., & Pelletier, L. (2020). The CP110-CEP97-CEP290 module orchestrates a centriolar satellite dependent response to proteotoxic stress. bioRxiv.

Nagai, T., Mukoyama, S., Kagiwada, H., Goshima, N., & Mizuno, K. (2018). Cullin-3–KCTD10-mediated CEP97 degradation promotes primary cilium formation. Journal of cell science, 131(24), jcs219527.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
Request for COA

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Second antibody and isotype control

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