Mouse Anti-CERCAM Recombinant Antibody (CBWJC-2505) (CBMAB-C3576WJ)

Name: Mouse Anti-CERCAM Recombinant Antibody (CBWJC-2505)
SKU: CBMAB-C3576WJ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBWJC-2505

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 186-207 within an internal region of CERCAM of human.

Host Species

Mouse

Specificity

Mouse, Rat, Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Cerebral Endothelial Cell Adhesion Molecule

Introduction

CERCAM (Cerebral Endothelial Cell Adhesion Molecule) is a Protein Coding gene. An important paralog of this gene is COLGALT1.

Entrez Gene ID

Human	51148
Mouse	99151
Rat	296616

UniProt ID

Human	Q5T4B2
Mouse	A3KGW5
Rat	Q5U309

Alternative Names

Cerebral Endothelial Cell Adhesion Molecule; Glycosyltransferase 25 Domain Containing 3; Cerebral Cell Adhesion Molecule; CEECAM1; GLT25D3; Probable Inactive Glycosyltransferase 25 Family Member 3;

Function

Probable cell adhesion protein involved in leukocyte transmigration across the blood-brain barrier. Does not express any beta-galactosyltransferase activity in vitro.

Biological Process

Cell adhesion Source: MGI
Leukocyte cell-cell adhesion Source: ProtInc
Protein phosphopantetheinylation Source: GOC

Cellular Location

Endoplasmic reticulum lumen

Zuo, Y., Xu, X., Chen, M., & Qi, L. (2021). The oncogenic role of the cerebral endothelial cell adhesion molecule (CERCAM) in bladder cancer cells in vitro and in vivo. Cancer Medicine.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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