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Mouse Anti-CH25H Recombinant Antibody (1G8) (CBMAB-C4201-LY)

This product is antibody recognizes CH25H. The antibody 1G8 immunoassay techniques such as: WB, IP, ELISA.
See all CH25H antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
1G8
Antibody Isotype
IgG
Application
WB, IP, ELISA

Basic Information

Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
cholesterol 25-hydroxylase
Introduction
This is an intronless gene that is involved in cholesterol and lipid metabolism. The encoded protein is a membrane protein and contains clusters of histidine residues essential for catalytic activity. Unlike most other sterol hydroxylases, this enzyme is a member of a small family of enzymes that utilize diiron cofactors to catalyze the hydroxylation of hydrophobic substrates. [provided by RefSeq, Jul 2008]
Entrez Gene ID
UniProt ID
Alternative Names
Cholesterol 25-Hydroxylase; Cholesterol 25-Monooxygenase; EC 1.14.99.38; H25OH; C25H;
Function
Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes (PubMed:9852097).
Plays a key role in cell positioning and movement in lymphoid tissues: 25-hydroxycholesterol is an intermediate in biosynthesis of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC), an oxysterol that acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for a number of lymphoid cells. May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. In testis, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation.
Biological Process
B cell chemotaxis Source: UniProtKB
Bile acid biosynthetic process Source: Reactome
Cholesterol metabolic process Source: GO_Central
Lipid metabolic process Source: ProtInc
Sterol biosynthetic process Source: GO_Central
Cellular Location
Endoplasmic reticulum membrane
PTM
N-glycosylated.

Madenspacher, J. H., Morrell, E. D., Gowdy, K. M., McDonald, J. G., Thompson, B. M., Muse, G., ... & Fessler, M. B. (2020). Cholesterol 25-hydroxylase promotes efferocytosis and resolution of lung inflammation. JCI insight, 5(11).

Russo, L., Muir, L., Geletka, L., Delproposto, J., Baker, N., Flesher, C., ... & Lumeng, C. N. (2020). Cholesterol 25-hydroxylase (CH25H) as a promoter of adipose tissue inflammation in obesity and diabetes. Molecular metabolism, 39, 100983.

Sheng, N., Ma, Z., Zhou, Y., Xu, J., Gao, Y., & Fu, X. Y. (2020). Cholesterol 25-hydroxylase protects against experimental colitis in mice by modulating epithelial gut barrier function. Scientific reports, 10(1), 1-12.

Yuan, Y., Wang, Z., Tian, B., Zhou, M., Fu, Z. F., & Zhao, L. (2019). Cholesterol 25-hydroxylase suppresses rabies virus infection by inhibiting viral entry. Archives of virology, 164(12), 2963-2974.

Zhang, Y., Song, Z., Wang, M., Lan, M., Zhang, K., Jiang, P., ... & Wang, X. (2019). Cholesterol 25-hydroxylase negatively regulates porcine intestinal coronavirus replication by the production of 25-hydroxycholesterol. Veterinary microbiology, 231, 129-138.

Liu, Y., Wei, Z., Ma, X., Yang, X., Chen, Y., Sun, L., ... & Duan, Y. (2018). 25-Hydroxycholesterol activates the expression of cholesterol 25-hydroxylase in an LXR-dependent mechanism [S]. Journal of lipid research, 59(3), 439-451.

Wang, J., Zeng, L., Zhang, L., Guo, Z. Z., Lu, S. F., Ming, S. L., ... & Chu, B. B. (2017). Cholesterol 25-hydroxylase acts as a host restriction factor on pseudorabies virus replication. Journal of General Virology, 98(6), 1467-1476.

Ke, W., Fang, L., Jing, H., Tao, R., Wang, T., Li, Y., ... & Xiao, S. (2017). Cholesterol 25-hydroxylase inhibits porcine reproductive and respiratory syndrome virus replication through enzyme activity-dependent and-independent mechanisms. Journal of virology, 91(19), e00827-17.

Li, Z., Martin, M., Zhang, J., Huang, H. Y., Bai, L., Zhang, J., ... & Shyy, J. Y. J. (2017). Krüppel-like factor 4 regulation of cholesterol-25-hydroxylase and liver X receptor mitigates atherosclerosis susceptibility. Circulation, 136(14), 1315-1330.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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