Mouse Anti-CH25H Recombinant Antibody (1G8) (CBMAB-C4201-LY)

Name: Mouse Anti-CH25H Recombinant Antibody (1G8)
SKU: CBMAB-C4201-LY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CH25H Recombinant Antibody (1G8) (CBMAB-C4201-LY) in WB

CH25H expression was detected by western blotting after UV irradiation. ...View More

Wang, Z., Lv, J., Yu, P., Qu, Y., Zhou, Y., Zhou, L., ... & Huang, B. (2022). SARS-CoV-2 treatment effects induced by ACE2-expressing microparticles are explained by the oxidized cholesterol-increased endosomal pH of alveolar macrophages. Cellular & Molecular Immunology, 19(2), 210-221.

Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CH25H Recombinant Antibody (1G8) (CBMAB-C4201-LY) in WB

The relative protein expression of CH25H was significantly upregulated (p < 0.05) but that of CYP7B1 was unaffected (p> 0.05) by metformin in the liver of DIO mice. ...View More

Guo, W. R., Liu, J., Cheng, L. D., Liu, Z. Y., Zheng, X. B., Liang, H., & Xu, F. (2021). Metformin alleviates steatohepatitis in diet-induced obese mice in a SIRT1-dependent way. Frontiers in Pharmacology, 12, 704112.

Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CH25H Recombinant Antibody (1G8) (CBMAB-C4201-LY) in WB

CH25H, CYP7B1, and RORα expression in catilage were analyzed via western blotting. The band intensity was quantified by normalizing to β-actin (n = 3). ...View More

Li, X., Zhang, L., Shi, X., Liao, T., Zhang, N., Gao, Y., ... & Wang, P. (2021). MicroRNA-10a-3p improves cartilage degeneration by regulating CH25H-CYP7B1-RORα mediated cholesterol metabolism in knee osteoarthritis rats. Frontiers in Pharmacology, 12, 690181.

Distributed under Open Access license CC BY 4.0, without modification.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

1G8

Application

WB, IP, ELISA

Immunogen

Amino acids 142-247 of CH25H of human.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.1 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

cholesterol 25-hydroxylase

Introduction

This is an intronless gene that is involved in cholesterol and lipid metabolism. The encoded protein is a membrane protein and contains clusters of histidine residues essential for catalytic activity. Unlike most other sterol hydroxylases, this enzyme is a member of a small family of enzymes that utilize diiron cofactors to catalyze the hydroxylation of hydrophobic substrates. [provided by RefSeq, Jul 2008]

Entrez Gene ID

9023

UniProt ID

O95992

Alternative Names

Cholesterol 25-Hydroxylase; Cholesterol 25-Monooxygenase; EC 1.14.99.38; H25OH; C25H;

Function

Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes (PubMed:9852097).
Plays a key role in cell positioning and movement in lymphoid tissues: 25-hydroxycholesterol is an intermediate in biosynthesis of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC), an oxysterol that acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for a number of lymphoid cells. May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. In testis, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation.

Biological Process

B cell chemotaxis Source: UniProtKB
Bile acid biosynthetic process Source: Reactome
Cholesterol metabolic process Source: GO_Central
Lipid metabolic process Source: ProtInc
Sterol biosynthetic process Source: GO_Central

Cellular Location

Endoplasmic reticulum membrane

PTM

N-glycosylated.

Madenspacher, J. H., Morrell, E. D., Gowdy, K. M., McDonald, J. G., Thompson, B. M., Muse, G., ... & Fessler, M. B. (2020). Cholesterol 25-hydroxylase promotes efferocytosis and resolution of lung inflammation. JCI insight, 5(11).

Russo, L., Muir, L., Geletka, L., Delproposto, J., Baker, N., Flesher, C., ... & Lumeng, C. N. (2020). Cholesterol 25-hydroxylase (CH25H) as a promoter of adipose tissue inflammation in obesity and diabetes. Molecular metabolism, 39, 100983.

Sheng, N., Ma, Z., Zhou, Y., Xu, J., Gao, Y., & Fu, X. Y. (2020). Cholesterol 25-hydroxylase protects against experimental colitis in mice by modulating epithelial gut barrier function. Scientific reports, 10(1), 1-12.

Yuan, Y., Wang, Z., Tian, B., Zhou, M., Fu, Z. F., & Zhao, L. (2019). Cholesterol 25-hydroxylase suppresses rabies virus infection by inhibiting viral entry. Archives of virology, 164(12), 2963-2974.

Zhang, Y., Song, Z., Wang, M., Lan, M., Zhang, K., Jiang, P., ... & Wang, X. (2019). Cholesterol 25-hydroxylase negatively regulates porcine intestinal coronavirus replication by the production of 25-hydroxycholesterol. Veterinary microbiology, 231, 129-138.

Liu, Y., Wei, Z., Ma, X., Yang, X., Chen, Y., Sun, L., ... & Duan, Y. (2018). 25-Hydroxycholesterol activates the expression of cholesterol 25-hydroxylase in an LXR-dependent mechanism [S]. Journal of lipid research, 59(3), 439-451.

Wang, J., Zeng, L., Zhang, L., Guo, Z. Z., Lu, S. F., Ming, S. L., ... & Chu, B. B. (2017). Cholesterol 25-hydroxylase acts as a host restriction factor on pseudorabies virus replication. Journal of General Virology, 98(6), 1467-1476.

Ke, W., Fang, L., Jing, H., Tao, R., Wang, T., Li, Y., ... & Xiao, S. (2017). Cholesterol 25-hydroxylase inhibits porcine reproductive and respiratory syndrome virus replication through enzyme activity-dependent and-independent mechanisms. Journal of virology, 91(19), e00827-17.

Li, Z., Martin, M., Zhang, J., Huang, H. Y., Bai, L., Zhang, J., ... & Shyy, J. Y. J. (2017). Krüppel-like factor 4 regulation of cholesterol-25-hydroxylase and liver X receptor mitigates atherosclerosis susceptibility. Circulation, 136(14), 1315-1330.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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