Sign in or Register   Sign in or Register
  |  

Mouse Anti-CHCHD4 Recombinant Antibody (CBYY-C1959) (CBMAB-C3397-YY)

This product is mouse antibody that recognizes CHCHD4. The antibody CBYY-C1959 can be used for immunoassay techniques such as: ELISA, WB
See all CHCHD4 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYY-C1959
Antibody Isotype
IgG2a, κ
Application
ELISA, WB

Basic Information

Immunogen
CHCHD4 (AAH33775.1, 1 a.a. ~ 142 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Preservative
PBS
Concentration
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
coiled-coil-helix-coiled-coil-helix domain containing 4
Entrez Gene ID
131474
UniProt ID
Q8N4Q1
Alternative Names
Thy-1 Cell Surface Antigen; Thy-1 Antigen; CDw90; Thy-1 Membrane Glycoprotein; Thy-1 T-Cell Antigen; CD90 Antigen; CD90;
Function
Central component of a redox-sensitive mitochondrial intermembrane space import machinery which is required for the biogenesis of respiratory chain complexes (PubMed:26004228).
Functions as chaperone and catalyzes the formation of disulfide bonds in substrate proteins, such as COX17, COX19, MICU1 and COA7 (PubMed:16185709, PubMed:26387864, PubMed:19182799, PubMed:21059946, PubMed:23186364, PubMed:23676665, PubMed:30885959).
Required for the import and folding of small cysteine-containing proteins (small Tim) in the mitochondrial intermembrane space (IMS). Required for the import of COA7 in the IMS (PubMed:30885959).
Precursor proteins to be imported into the IMS are translocated in their reduced form into the mitochondria. The oxidized form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with the reduced precursor protein, resulting in oxidation of the precursor protein that now contains an intramolecular disulfide bond and is able to undergo folding in the IMS (PubMed:16185709, PubMed:19182799, PubMed:21059946, PubMed:23676665).
Reduced CHCHD4/MIA40 is then reoxidized by GFER/ERV1 via a disulfide relay system (PubMed:23186364).
Mediates formation of disulfide bond in MICU1 in the IMS, promoting formation of the MICU1-MICU2 heterodimer that regulates mitochondrial calcium uptake (PubMed:26387864).
Biological Process
de novo' posttranslational protein folding Source: UniProtKB
Mitochondrial respiratory chain complex assembly Source: UniProtKB
Peptidyl-cysteine oxidation Source: UniProtKB
Protein import into mitochondrial intermembrane space Source: UniProtKB
Protein maturation by protein folding Source: UniProtKB
Cellular Location
Mitochondrion intermembrane space
PTM
Forms intrachain disulfide bridges, but exists in different redox states.
Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-CHCHD4 Recombinant Antibody (CBYY-C1959)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare