Mouse Anti-CHCHD4 Recombinant Antibody (CBYY-C1959) (CBMAB-C3397-YY)

Name: Mouse Anti-CHCHD4 Recombinant Antibody (CBYY-C1959)
SKU: CBMAB-C3397-YY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYY-C1959

Application

ELISA, WB

Immunogen

CHCHD4 full-length recombinant protein.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

coiled-coil-helix-coiled-coil-helix domain containing 4

Entrez Gene ID

131474

UniProt ID

Q8N4Q1

Alternative Names

Thy-1 Cell Surface Antigen; Thy-1 Antigen; CDw90; Thy-1 Membrane Glycoprotein; Thy-1 T-Cell Antigen; CD90 Antigen; CD90;

Function

Central component of a redox-sensitive mitochondrial intermembrane space import machinery which is required for the biogenesis of respiratory chain complexes (PubMed:26004228).
Functions as chaperone and catalyzes the formation of disulfide bonds in substrate proteins, such as COX17, COX19, MICU1 and COA7 (PubMed:16185709, PubMed:26387864, PubMed:19182799, PubMed:21059946, PubMed:23186364, PubMed:23676665, PubMed:30885959).
Required for the import and folding of small cysteine-containing proteins (small Tim) in the mitochondrial intermembrane space (IMS). Required for the import of COA7 in the IMS (PubMed:30885959).
Precursor proteins to be imported into the IMS are translocated in their reduced form into the mitochondria. The oxidized form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with the reduced precursor protein, resulting in oxidation of the precursor protein that now contains an intramolecular disulfide bond and is able to undergo folding in the IMS (PubMed:16185709, PubMed:19182799, PubMed:21059946, PubMed:23676665).
Reduced CHCHD4/MIA40 is then reoxidized by GFER/ERV1 via a disulfide relay system (PubMed:23186364).
Mediates formation of disulfide bond in MICU1 in the IMS, promoting formation of the MICU1-MICU2 heterodimer that regulates mitochondrial calcium uptake (PubMed:26387864).

Biological Process

de novo' posttranslational protein folding Source: UniProtKB
Mitochondrial respiratory chain complex assembly Source: UniProtKB
Peptidyl-cysteine oxidation Source: UniProtKB
Protein import into mitochondrial intermembrane space Source: UniProtKB
Protein maturation by protein folding Source: UniProtKB

Cellular Location

Mitochondrion intermembrane space

PTM

Forms intrachain disulfide bridges, but exists in different redox states.

Al-Habib, H., & Ashcroft, M. (2021). CHCHD4 (MIA40) and the mitochondrial disulfide relay system. Biochemical Society Transactions, 49(1), 17-27.

Thomas, L. W., Stephen, J. M., Esposito, C., Hoer, S., Antrobus, R., Ahmed, A., ... & Ashcroft, M. (2019). CHCHD4 confers metabolic vulnerabilities to tumour cells through its control of the mitochondrial respiratory chain. Cancer & metabolism, 7(1), 1-18.

Thomas, L. W., Esposito, C., Stephen, J. M., Costa, A. S., Frezza, C., Blacker, T. S., ... & Ashcroft, M. (2019). CHCHD4 regulates tumour proliferation and EMT-related phenotypes, through respiratory chain-mediated metabolism. Cancer & metabolism, 7(1), 1-17.

Thomas, L. W., Esposito, C., Stephen, J. M., Costa, A. S., Frezza, C., Blacker, T. S., ... & Ashcroft, M. (2019). CHCHD4 regulates a proliferation-EMT switch in tumour cells, through respiratory complex I mediated metabolism. bioRxiv, 513531.

Thomas, L. W., Staples, O., Turmaine, M., & Ashcroft, M. (2019). Corrigendum: CHCHD4 Regulates Intracellular Oxygenation and Perinuclear Distribution of Mitochondria. Frontiers in oncology, 9, 23.

Thomas, L. W., Staples, O., Turmaine, M., & Ashcroft, M. (2017). CHCHD4 regulates intracellular oxygenation and perinuclear distribution of mitochondria. Frontiers in oncology, 7, 71.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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