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Rat Anti-CHD5 Recombinant Antibody (5A10) (CBMAB-C7255-LY)

This product is antibody recognizes CHD5. The antibody 5A10 immunoassay techniques such as: ICC, IF, WB.
See all CHD5 antibodies

Summary

Host Animal
Rat
Specificity
Human
Clone
5A10
Antibody Isotype
IgG2a
Application
ICC, IF, WB

Basic Information

Immunogen
This antibody was raised against a peptide within the C-terminal region of mouse Chd5
Specificity
Human
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Concentration
1 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Chromodomain Helicase DNA Binding Protein 5
Introduction
This gene encodes a member of the chromodomain helicase DNA-binding protein family. Members of this family are characterized by a chromodomain, a helicase ATP-binding domain and an additional functional domain. This gene encodes a neuron-specific protein that may function in chromatin remodeling and gene transcription. This gene is a potential tumor suppressor gene that may play a role in the development of neuroblastoma. [provided by RefSeq, Feb 2012]
Entrez Gene ID
26038
UniProt ID
Q8TDI0
Alternative Names
Chromodomain Helicase DNA Binding Protein 5; ATP-Dependent Helicase CHD5; CHD-5; Chromodomain-Helicase-DNA-Binding Protein 5; EC 3.6.4.12; KIAA0444; EC 3.6.1;
Function
Chromatin-remodeling protein that binds DNA through histones and regulates gene transcription. May specifically recognize and bind trimethylated 'Lys-27' (H3K27me3) and non-methylated 'Lys-4' of histone H3. Plays a role in the development of the nervous system by activating the expression of genes promoting neuron terminal differentiation. In parallel, it may also positively regulate the trimethylation of histone H3 at 'Lys-27' thereby specifically repressing genes that promote the differentiation into non-neuronal cell lineages. Tumor suppressor, it regulates the expression of genes involved in cell proliferation and differentiation. Downstream activated genes may include CDKN2A that positively regulates the p53/TP53 pathway, which in turn, prevents cell proliferation. In spermatogenesis, it probably regulates histone hyperacetylation and the replacement of histones by transition proteins in chromatin, a crucial step in the condensation of spermatid chromatin and the production of functional spermatozoa.
Biological Process
Cerebral cortex neuron differentiation Source: UniProtKB
Histone H3-K27 trimethylation Source: UniProtKB
Histone H4 acetylation Source: UniProtKB
Negative regulation of cell population proliferation Source: UniProtKB
Positive regulation of signal transduction by p53 class mediator Source: UniProtKB
Regulation of transcription by RNA polymerase II Source: GO_Central
Spermatogenesis, exchange of chromosomal proteins Source: UniProtKB
Cellular Location
Nucleus. Associates with heterochromatin.
Involvement in disease
Defects in CHD5 may be a cause of the development of cancers from epithelial, neural and hematopoietic origin. CHD5 is one of the missing genes in the del(1p36), a deletion which is extremely common in this type of cancers. A decrease of its expression, results in increased susceptibility of cells to Ras-mediated transformation in vitro and in vivo (PubMed:17289567).
PTM
Methylated at Gln-1390 by N6AMT1.

Parenti, I., Lehalle, D., Nava, C., Torti, E., Leitão, E., Person, R., ... & Mignot, C. (2021). Missense and truncating variants in CHD5 in a dominant neurodevelopmental disorder with intellectual disability, behavioral disturbances, and epilepsy. Human genetics, 1-12.

Huang, S., Yan, Q., Xiong, S., Peng, Y., Zhao, R., & Liu, C. (2020). Chromodomain Helicase DNA-Binding Protein 5 Inhibits Renal Cell Carcinoma Tumorigenesis by Activation of the p53 and RB Pathways. BioMed Research International, 2020.

Hashimoto, T., Kurokawa, Y., Wada, N., Takahashi, T., Miyazaki, Y., Tanaka, K., ... & Doki, Y. (2020). Clinical significance of chromatin remodeling factor CHD5 expression in gastric cancer. Oncology letters, 19(1), 1066-1073.

Zhou, Y., Qiu, Y. E., Qin, S. L., Luo, Y., Cui, R., Qin, J., ... & Shi, X. Y. (2018). Expression of CHD5 may serve as an independent biomarker of prognosis in colorectal cancer via immunohistochemical staining. International journal of clinical and experimental pathology, 11(5), 2792.

Zhu, X., Yu, H., Xiao, Q., Ke, J., Li, H., Chen, Z., ... & Luo, H. (2018). Genetic variations in chromodomain helicase DNA-binding protein 5, gene-environment interactions and risk of sporadic Alzheimer’s disease in Chinese population. Oncotarget, 9(38), 24872.

Liu, Z., Su, D., Qi, X., & Ma, J. (2018). MiR‑500a‑5p promotes glioblastoma cell proliferation, migration and invasion by targeting chromodomain helicase DNA binding protein 5. Molecular medicine reports, 18(3), 2689-2696.

Zhu, X., Kong, Q., Xie, L., Chen, Z., Li, H., Zhu, Z., ... & Luo, H. (2018). The single-nucleotide polymorphisms in CHD5 affect the prognosis of patients with hepatocellular carcinoma. Oncotarget, 9(17), 13222.

Pisansky, M. T., Young, A. E., O'connor, M. B., Gottesman, I. I., Bagchi, A., & Gewirtz, J. C. (2017). Mice lacking the chromodomain helicase DNA-binding 5 chromatin remodeler display autism-like characteristics. Translational psychiatry, 7(6), e1152-e1152.

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For research use only. Not intended for any clinical use.

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