Mouse Anti-CHERP Recombinant Antibody (CBFYC-1821) (CBMAB-C1884-FY)

Name: Mouse Anti-CHERP Recombinant Antibody (CBFYC-1821)
SKU: CBMAB-C1884-FY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CHERP Recombinant Antibody (CBFYC-1821) (CBMAB-C1884-FY) in WB and IP

Co-immunoprecipitation (IP) of endogenous CHERP and ALG-2. CHERP was extracted from the P0.6 fraction by brief sonication with lysis buffer T (50 mm Tris-HCl, pH 7.5, 150 mm NaCl, 1.5 mm MgCl2, and 0.2% Triton X-100 containing protease inhibitors). ...View More

Sasaki-Osugi, K., Imoto, C., Takahara, T., Shibata, H., & Maki, M. (2013). Nuclear ALG-2 protein interacts with Ca2+ homeostasis endoplasmic reticulum protein (CHERP) Ca2+-dependently and participates in regulation of alternative splicing of inositol trisphosphate receptor type 1 (IP3R1) pre-mRNA. Journal of Biological Chemistry, 288(46), 33361-33375.

Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CHERP Recombinant Antibody (CBFYC-1821) (CBMAB-C1884-FY) in IF

Localization of endogenous CHERP at nuclear speckles by immunofluorescence microscopic analysis. HeLa cells were cultured on coverslips, fixed, and processed for pre-permeabilization with 50 μg/ml digitonin in buffer D (20 mm HEPES-KOH, pH 7.6, 50 mm NaCl, 2 mm MgCl2, 250 mm sucrose, 1 mm DTT) (e-h) on ice for 10 min and washed with buffer D or unprocessed for pre-permeabilization (a-d), fixed in 4% paraformaldehyde in PBS, and permeabilized with 0.1% Triton X-100. Then, the cells were triple-immunostained with goat anti-ALG-2 pAb (green), rabbit anti-CHERP pAb (red), and mouse anti-SC35 mAb (blue). The fluorescence signals were analyzed with a confocal laser-scanning microscope and are represented in black and white. Merged images are shown in d and h in color. The small boxed areas are magnified in the respective large boxed areas. Bars, 5 μm. ...View More

Distributed under Open Access license CC BY 4.0, without modification.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYC-1821

Application

ELISA, WB

Immunogen

CHERP (795 a.a. ~ 883 a.a) partial recombinant protein.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 795-883

More Infomation

Target

Full Name

calcium homeostasis endoplasmic reticulum protein

Introduction

CHERP (Calcium Homeostasis Endoplasmic Reticulum Protein) is a Protein Coding gene. Diseases associated with CHERP include Autosomal Dominant Cerebellar Ataxia. Among its related pathways are Gene Expression and mRNA Splicing-Major Pathway. Gene Ontology (GO) annotations related to this gene include nucleic acid binding and RNA binding.

Entrez Gene ID

10523

UniProt ID

Q8IWX8

Alternative Names

Calcium Homeostasis Endoplasmic Reticulum Protein; ERPROT 213-21; SCAF6; SR-Related CTD Associated Factor 6; SR-Related CTD-Associated Factor 6; Protein With Polyglutamine Repeat

Function

Involved in calcium homeostasis, growth and proliferation.

Biological Process

Cellular calcium ion homeostasis Source: MGI
mRNA splicing, via spliceosome Source: Reactome
Negative regulation of cell population proliferation Source: MGI
Nervous system development Source: ProtInc
Positive regulation of calcineurin-NFAT signaling cascade Source: UniProtKB
Release of sequestered calcium ion into cytosol Source: UniProtKB

Cellular Location

Endoplasmic reticulum; Cytoplasm; Perinuclear region. Distributed throughout the cytoplasm and also localizes to the perinuclear region of both human erythroleukemia (HEL) cells and Jurkat cells. Colocalizes with ITPR1.

Wang, Q., Wang, Y., Liu, Y., Zhang, C., Luo, Y., Guo, R., ... & Feng, Y. (2019). U2‐related proteins CHERP and SR140 contribute to colorectal tumorigenesis via alternative splicing regulation. International journal of cancer, 145(10), 2728-2739.

Pitts, M. W., & Hoffmann, P. R. (2018). Endoplasmic reticulum-resident selenoproteins as regulators of calcium signaling and homeostasis. Cell Calcium, 70, 76-86.

Zhang, D., Wang, F., Pang, Y., Ke, X. X., Zhu, S., Zhao, E., ... & Cui, H. (2017). Down-regulation of CHERP inhibits neuroblastoma cell proliferation and induces apoptosis through ER stress induction. Oncotarget, 8(46), 80956.

Wang, C., Li, R., Huang, Y., Wang, M., Yang, F., Huang, D., ... & Cheng, J. (2017). Selenoprotein K modulate intracellular free Ca2+ by regulating expression of calcium homoeostasis endoplasmic reticulum protein. Biochemical and biophysical research communications, 484(4), 734-739.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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