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Mouse Anti-CHERP Recombinant Antibody (CBFYC-1821) (CBMAB-C1884-FY)


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Published Data
CBMAB-C1884-FY-1
Mouse Anti-CHERP Recombinant Antibody (CBFYC-1821) (CBMAB-C1884-FY) in WB and IP
Co-immunoprecipitation (IP) of endogenous CHERP and ALG-2. CHERP was extracted from the P0.6 fraction by brief sonication with lysis buffer T (50 mm Tris-HCl, pH 7.5, 150 mm NaCl, 1.5 mm MgCl2, and 0.2% Triton X-100 containing protease inhibitors). ...View More
Sasaki-Osugi, K., Imoto, C., Takahara, T., Shibata, H., & Maki, M. (2013). Nuclear ALG-2 protein interacts with Ca2+ homeostasis endoplasmic reticulum protein (CHERP) Ca2+-dependently and participates in regulation of alternative splicing of inositol trisphosphate receptor type 1 (IP3R1) pre-mRNA. Journal of Biological Chemistry, 288(46), 33361-33375.
Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-C1884-FY-2
Mouse Anti-CHERP Recombinant Antibody (CBFYC-1821) (CBMAB-C1884-FY) in IF
Localization of endogenous CHERP at nuclear speckles by immunofluorescence microscopic analysis. HeLa cells were cultured on coverslips, fixed, and processed for pre-permeabilization with 50 μg/ml digitonin in buffer D (20 mm HEPES-KOH, pH 7.6, 50 mm NaCl, 2 mm MgCl2, 250 mm sucrose, 1 mm DTT) (e-h) on ice for 10 min and washed with buffer D or unprocessed for pre-permeabilization (a-d), fixed in 4% paraformaldehyde in PBS, and permeabilized with 0.1% Triton X-100. Then, the cells were triple-immunostained with goat anti-ALG-2 pAb (green), rabbit anti-CHERP pAb (red), and mouse anti-SC35 mAb (blue). The fluorescence signals were analyzed with a confocal laser-scanning microscope and are represented in black and white. Merged images are shown in d and h in color. The small boxed areas are magnified in the respective large boxed areas. Bars, 5 μm. ...View More
Sasaki-Osugi, K., Imoto, C., Takahara, T., Shibata, H., & Maki, M. (2013). Nuclear ALG-2 protein interacts with Ca2+ homeostasis endoplasmic reticulum protein (CHERP) Ca2+-dependently and participates in regulation of alternative splicing of inositol trisphosphate receptor type 1 (IP3R1) pre-mRNA. Journal of Biological Chemistry, 288(46), 33361-33375.
Distributed under Open Access license CC BY 4.0, without modification.

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYC-1821
Antibody Isotype
IgG2b, κ
Application
ELISA, WB

Basic Information

Immunogen
CHERP (795 a.a. ~ 883 a.a) partial recombinant protein.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
None
Concentration
Batch dependent
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 795-883

Target

Full Name
calcium homeostasis endoplasmic reticulum protein
Introduction
CHERP (Calcium Homeostasis Endoplasmic Reticulum Protein) is a Protein Coding gene. Diseases associated with CHERP include Autosomal Dominant Cerebellar Ataxia. Among its related pathways are Gene Expression and mRNA Splicing-Major Pathway. Gene Ontology (GO) annotations related to this gene include nucleic acid binding and RNA binding.
Entrez Gene ID
10523
UniProt ID
Q8IWX8
Alternative Names
Calcium Homeostasis Endoplasmic Reticulum Protein; ERPROT 213-21; SCAF6; SR-Related CTD Associated Factor 6; SR-Related CTD-Associated Factor 6; Protein With Polyglutamine Repeat
Function
Involved in calcium homeostasis, growth and proliferation.
Biological Process
Cellular calcium ion homeostasis Source: MGI
mRNA splicing, via spliceosome Source: Reactome
Negative regulation of cell population proliferation Source: MGI
Nervous system development Source: ProtInc
Positive regulation of calcineurin-NFAT signaling cascade Source: UniProtKB
Release of sequestered calcium ion into cytosol Source: UniProtKB
Cellular Location
Endoplasmic reticulum; Cytoplasm; Perinuclear region. Distributed throughout the cytoplasm and also localizes to the perinuclear region of both human erythroleukemia (HEL) cells and Jurkat cells. Colocalizes with ITPR1.
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For research use only. Not intended for any clinical use.

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