Mouse Anti-CHGA Recombinant Antibody (LK2H10) (CBMAB-0236CQ)

Name: Mouse Anti-CHGA Recombinant Antibody (LK2H10)
SKU: CBMAB-0236CQ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

LK2H10

Application

WB, IP, IF, IHC-P

Immunogen

Human pheochromocytoma.

Host Species

Mouse

Specificity

Human, Mouse, Rat, Pig, Monkey

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.1 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Chromogranin A

Introduction

Chromogranin A (CHGA) regulates catecholamine storage and release through intracellular (vesiculogenic) and extracellular (catecholamine release--inhibitory) mechanisms. The nucleotide sequence showed that human chromogranin A is a 439-residue protein preceded by an 18-residue signal peptide. Sequence findings suggested that pancreastatin is derived from chromogranin A itself rather than from a protein that is only similar to chromogranin A.

Entrez Gene ID

Human	1113
Mouse	12652
Rat	24258
Monkey	574388
Pig	397540

UniProt ID

Human	P10645
Mouse	P26339
Rat	P10354
Monkey	H2Q8T7
Pig	P04404

Alternative Names

Chromogranin A; Parathyroid Secretory Protein 1; Pituitary Secretory Protein I; SP-I; CGA; Betagranin (N-Terminal Fragment Of Chromogranin A); Pancreastatin; Chromofungin; Vasostatin; Parastatin; Catestatin

Function

Pancreastatin:
Strongly inhibits glucose induced insulin release from the pancreas.
Catestatin:
Inhibits catecholamine release from chromaffin cells and noradrenergic neurons by acting as a non-competitive nicotinic cholinergic antagonist (PubMed:15326220).
Displays antibacterial activity against Gram-positive bacteria S.aureus and M.luteus, and Gram-negative bacteria E.coli and P.aeruginosa (PubMed:15723172 and PubMed:24723458).
Can induce mast cell migration, degranulation and production of cytokines and chemokines (PubMed:21214543).
Acts as a potent scavenger of free radicals in vitro (PubMed:24723458).
May play a role in the regulation of cardiac function and blood pressure (PubMed:18541522).
Serpinin:
Regulates granule biogenesis in endocrine cells by up-regulating the transcription of protease nexin 1 (SERPINE2) via a cAMP-PKA-SP1 pathway. This leads to inhibition of granule protein degradation in the Golgi complex which in turn promotes granule formation.

Biological Process

Adenylate cyclase-activating adrenergic receptor signaling pathway involved in cardiac muscle relaxation Source: BHF-UCL
Antimicrobial humoral response Source: Reactome
Defense response to bacterium Source: GO_Central
Defense response to fungus Source: UniProtKB-KW
Defense response to Gram-negative bacterium Source: UniProtKB
Defense response to Gram-positive bacterium Source: UniProtKB
Innate immune response Source: UniProtKB
Killing of cells of other organism Source: UniProtKB-KW
Mast cell activation Source: UniProtKB
Mast cell chemotaxis Source: UniProtKB
Mast cell degranulation Source: UniProtKB
Negative regulation of catecholamine secretion Source: UniProtKB
Negative regulation of insulin secretion Source: GO_Central
Negative regulation of neuron death Source: BHF-UCL
Organelle organization Source: BHF-UCL
Positive regulation of cardiac muscle contraction Source: BHF-UCL
Positive regulation of dense core granule biogenesis Source: UniProtKB
Positive regulation of phospholipase C-activating G protein-coupled receptor signaling pathway Source: BHF-UCL
Positive regulation of relaxation of cardiac muscle Source: BHF-UCL
Protein localization to secretory granule Source: Ensembl
Regulation of blood pressure Source: ProtInc
Regulation of the force of heart contraction Source: BHF-UCL

Cellular Location

Serpinin: Secreted; secretory vesicle. Pyroglutaminated serpinin localizes to secretory vesicle.
Secreted; Secretory vesicle; Neuronal dense core vesicle. Associated with the secretory granule membrane through direct interaction to SCG3 that in turn binds to cholesterol-enriched lipid rafts in intragranular conditions. In pituitary gonadotropes, located in large secretory granules.

PTM

Sulfated on tyrosine residues and/or contains sulfated glycans.
O-glycosylated with core 1 or possibly core 8 glycans.
Proteolytic processing gives rise to an additional longer form of catestatin (residues 358-390) which displays a less potent catecholamine release-inhibitory activity (PubMed:10781584). Plasmin-mediated proteolytic processing can give rise to additional shorter and longer forms of catestatin peptides (PubMed:17991725).

Bralewska, M., Biesiada, L., Grzesiak, M., Rybak-Krzyszkowska, M., Huras, H., Gach, A., ... & Sakowicz, A. (2021). Chromogranin A demonstrates higher expression in preeclamptic placentas than in normal pregnancy. BMC Pregnancy and Childbirth, 21(1), 1-10.

Eissa, N., Hussein, H., Tshikudi, D. M., Hendy, G. N., Bernstein, C. N., & Ghia, J. E. (2020). Interdependence between Chromogranin-A, Alternatively Activated Macrophages, Tight Junction Proteins and the Epithelial Functions. A Human and In-Vivo/In-Vitro Descriptive Study. International Journal of Molecular Sciences, 21(21), 7976.

Mahata, S. K., & Corti, A. (2019). Chromogranin A and its fragments in cardiovascular, immunometabolic, and cancer regulation. Annals of the New York Academy of Sciences, 1455(1), 34.

Zhang, X., Zhang, H., Shen, B., & Sun, X. F. (2019). Chromogranin-A expression as a novel biomarker for early diagnosis of colon cancer patients. International journal of molecular sciences, 20(12), 2919.

Guo, Z., Wang, Y., Xiang, S., Wang, S., & Chan, F. L. (2019). Chromogranin A is a predictor of prognosis in patients with prostate cancer: a systematic review and meta-analysis. Cancer management and research, 11, 2747.

Eissa, N., Hussein, H., Kermarrec, L., Ali, A. Y., Marshall, A., Metz-Boutigue, M. H., ... & Ghia, J. E. (2018). Chromogranin-A regulates macrophage function and the apoptotic pathway in murine DSS colitis. Journal of Molecular Medicine, 96(2), 183-198.

Eissa, N., Hussein, H., Hendy, G. N., Bernstein, C. N., & Ghia, J. E. (2018). Chromogranin-A and its derived peptides and their pharmacological effects during intestinal inflammation. Biochemical pharmacology, 152, 315-326.

Wollam, J., Mahata, S., Riopel, M., Hernandez-Carretero, A., Biswas, A., Bandyopadhyay, G. K., ... & Mahata, S. K. (2017). Chromogranin A regulates vesicle storage and mitochondrial dynamics to influence insulin secretion. Cell and tissue research, 368(3), 487-501.

Bandyopadhyay, G. K., & Mahata, S. K. (2017). Chromogranin A regulation of obesity and peripheral insulin sensitivity. Frontiers in endocrinology, 8, 20.

Subramanian, L., Khan, A. A., Allu, P. K., Kiranmayi, M., Sahu, B. S., Sharma, S., ... & Mahapatra, N. R. (2017). A haplotype variant of the human chromogranin A gene (CHGA) promoter increases CHGA expression and the risk for cardiometabolic disorders. Journal of Biological Chemistry, 292(34), 13970-13985.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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