Mouse Anti-CHKB Recombinant Antibody (EG675) (CBMAB-EN816-LY)

Name: Mouse Anti-CHKB Recombinant Antibody (EG675)
SKU: CBMAB-EN816-LY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CHKB Recombinant Antibody (EG675) (CBMAB-EN816-LY) in WB

Western blot of hindlimb (quadriceps) samples from three distinct (lanes 1-3) Chkb+/+, four distinct (lanes 4-7) Chkb+/− and three distinct (lanes 8-10) Chkb−/− mice probed with anti-Para, anti- Pparb, anti-Pparg, anti-Cpt1b, and anti-Gapdh antibodies. Bottom: densitometry of the western blot data shows the ratio of Ppara, Pparb, Pparg, and Cpt1b to Gapdh. ...View More

Tavasoli, M., Lahire, S., Sokolenko, S., Novorolsky, R., Reid, S. A., Lefsay, A., ... & McMaster, C. R. (2022). Mechanism of action and therapeutic route for a muscular dystrophy caused by a genetic defect in lipid metabolism. Nature Communications, 13(1), 1559.

Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CHKB Recombinant Antibody (EG675) (CBMAB-EN816-LY) in WB

Western blot of heart samples from three distinct (lanes 1-3) Chkb+/+, four distinct (lanes 4-7) Chkb+/−, and three distinct (lanes 8-10) Chkb−/− mice probed with anti-Chkb, anti-Chka, and anti-Gapdh antibodies. ...View More

Tavasoli, M., Feridooni, T., Feridooni, H., Sokolenko, S., Mishra, A., Lefsay, A., ... & McMaster, C. R. (2022). A mouse model of inherited choline kinase β-deficiency presents with specific cardiac abnormalities and a predisposition to arrhythmia. Journal of Biological Chemistry, 298(3).

Distributed under Open Access license CC BY 4.0, without modification.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

EG675

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 1-78 mapping at the N-terminus of ChoKB of mouse.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Choline Kinase Beta

Introduction

Choline kinase (CK) and ethanolamine kinase (EK) catalyze the phosphorylation of choline/ethanolamine to phosphocholine/phosphoethanolamine. This is the first enzyme in the biosynthesis of phosphatidylcholine/phosphatidylethanolamine in all animal cells. The highly purified CKs from mammalian sources and their recombinant gene products have been shown to have EK activity also, indicating that both activities reside on the same protein. The choline kinase-like protein encoded by CHKL belongs to the choline/ethanolamine kinase family; however, its exact function is not known. Read-through transcripts are expressed from this locus that include exons from the downstream CPT1B locus. [provided by RefSeq, Jun 2009]

Entrez Gene ID

1120

UniProt ID

Q9Y259

Alternative Names

Choline Kinase Beta; Choline Kinase-Like Protein; Ethanolamine Kinase Beta; CHETK; CKEKB; CHKL; CKB; EKB; CK;

Function

Has a key role in phospholipid metabolism, and catalyzes the first step of phosphatidylethanolamine and phosphatidylcholine biosynthesis.

Biological Process

Phosphatidylcholine biosynthetic process Source: Reactome
Phosphatidylethanolamine biosynthetic process Source: UniProtKB

Cellular Location

Cytosol

Involvement in disease

Muscular dystrophy, congenital, megaconial type (MDCMC): An autosomal recessive, congenital muscular dystrophy characterized by early-onset muscle wasting, mental retardation, and dilated cardiomyopathy in half of affected individuals. Some patients may die from cardiomyopathy in the first or second decade of life. Muscle biopsy shows peculiar enlarged mitochondria that are prevalent toward the periphery of the fibers but are sparse in the center.

Chen, X., Qiu, H., Wang, C., Yuan, Y., Tickner, J., Xu, J., & Zou, J. (2017). Molecular structure and differential function of choline kinases CHKα and CHKβ in musculoskeletal system and cancer. Cytokine & growth factor reviews, 33, 65-72.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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