Mouse Anti-CHMP4A Recombinant Antibody (CBWJC-2692) (CBMAB-C3785WJ)

Name: Mouse Anti-CHMP4A Recombinant Antibody (CBWJC-2692)
SKU: CBMAB-C3785WJ
Rating: 5 (1 reviews)

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Datasheet

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Basic Information

Host Animal

Mouse

Clone

CBWJC-2692

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 171-222 mapping at the C-terminus of CHMP4A of human.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Charged Multivesicular Body Protein 4A

Introduction

CHMP4A belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. These proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies (MVBs). Some CHMPs have both nuclear and cytoplasmic/vesicular distributions, and one such CHMP, CHMP1A (MIM 164010), is required for both MVB formation and regulation of cell cycle progression (Tsang et al., 2006 [PubMed 16730941]).[supplied by OMIM, Mar 2008]

Entrez Gene ID

29082

UniProt ID

Q9BY43

Alternative Names

Charged Multivesicular Body Protein 4A; Vacuolar Protein Sorting-Associated Protein 32-1; SNF7 Homolog Associated With Alix-2; Chromatin Modifying Protein 4A; C14orf123; VPS32-1; SNF7-1; SHAX2; Snf7 Homologue Associated With Alix 2; Chromosome 14 Open Reading Frame 123;

Function

Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. When overexpressed, membrane-assembled circular arrays of CHMP4A filaments can promote or stabilize negative curvature and outward budding. Via its interaction with PDCD6IP involved in HIV-1 p6- and p9-dependent virus release. CHMP4A/B/C are required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413).

Biological Process

Endosomal transport Source: Reactome
Late endosome to vacuole transport via multivesicular body sorting pathway Source: GO_Central
Macroautophagy Source: ParkinsonsUK-UCL
Membrane invagination Source: UniProtKB
Midbody abscission Source: UniProtKB
Mitotic metaphase plate congression Source: UniProtKB
Mitotic nuclear envelope reassembly Source: Reactome
Multivesicular body assembly Source: ParkinsonsUK-UCL
Negative regulation of autophagosome assembly Source: UniProtKB
Negative regulation of neuron death Source: UniProtKB
Nucleus organization Source: UniProtKB
Plasma membrane tubulation Source: UniProtKB
Posttranslational protein targeting to endoplasmic reticulum membrane Source: UniProtKB
Protein polymerization Source: UniProtKB
Vesicle budding from membrane Source: UniProtKB
Viral budding via host ESCRT complex Source: UniProtKB
Viral life cycle Source: Reactome

Cellular Location

Late endosome membrane; Cytoplasmic vesicle membrane. Membrane-associated. Localizes to large vesicle-like structures. Localizes to the midbody of dividing cells. Localized in two distinct rings on either side of the Fleming body.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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