Mouse Anti-CHMP6 Recombinant Antibody (CBXC-1748) (CBMAB-C5596-CQ)

Name: Mouse Anti-CHMP6 Recombinant Antibody (CBXC-1748)
SKU: CBMAB-C5596-CQ
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CHMP6 Recombinant Antibody (CBXC-1748) (CBMAB-C5596-CQ) in IF

i3Neurons expressing the guides indicated were fixed and labeled for CHMP6, then visualized by confocal immunofluorescence microscopy. The number of CHMP6 puncta per cell was quantified in 3 biological repeats (≥20 cells per experimental condition in each repeat). Bars indicate mean ± SEM, p values calculated by one-way ANOVA with Tukey's correction for multiple testing. Micrograph scale bar = 10 μm. ...View More

Rodger, C., Flex, E., Allison, R. J., Sanchis-Juan, A., Hasenahuer, M. A., Cecchetti, S., ... & Reid, E. (2020). De novo VPS4A mutations cause multisystem disease with abnormal neurodevelopment. The American Journal of Human Genetics, 107(6), 1129-1148.

Distributed under Open Access license CC BY 4.0, without modification.

Datasheet

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Basic Information

Host Animal

Mouse

Clone

CBXC-1748

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 1-201 representing full length CHMP6 of human.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Charged Multivesicular Body Protein 6

Introduction

This gene encodes a member of the chromatin-modifying protein/charged multivesicular body protein family. Proteins in this family are part of the ESCRT-III (endosomal sorting complex required for transport III) which degrades surface receptors, and in biosynthesis of endosomes.

Entrez Gene ID

79643

UniProt ID

Q96FZ7

Alternative Names

Charged Multivesicular Body Protein 6; Vacuolar Protein Sorting-Associated Protein 20; Chromatin-Modifying Protein 6; HVps20; VPS20; Chromatin Modifying Protein 6;

Function

Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. In the ESCRT-III complex, it probably serves as an acceptor for the ESCRT-II complex on endosomal membranes.

Biological Process

Endosomal transport Source: Reactome
ESCRT III complex assembly Source: ParkinsonsUK-UCL
Late endosome to vacuole transport via multivesicular body sorting pathway Source: GO_Central
Macroautophagy Source: ParkinsonsUK-UCL
Midbody abscission Source: UniProtKB
Mitotic metaphase plate congression Source: UniProtKB
Mitotic nuclear envelope reassembly Source: Reactome
Multivesicular body assembly Source: ParkinsonsUK-UCL
Negative regulation of epidermal growth factor-activated receptor activity Source: UniProtKB
Nucleus organization Source: UniProtKB
Protein transport Source: UniProtKB-KW
Regulation of protein catabolic process Source: UniProtKB
Vesicle budding from membrane Source: GO_Central
Viral budding via host ESCRT complex Source: UniProtKB
Viral life cycle Source: Reactome

Cellular Location

Endosome membrane; Late endosome membrane; Endomembrane system; Membrane. Localizes to endosomal membranes.

PTM

ISGylated in a CHMP5-dependent manner. Isgylation weakens its interaction with VPS4A.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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