Mouse Anti-CHP2 Recombinant Antibody (1066) (CBMAB-1066CQ)

Name: Mouse Anti-CHP2 Recombinant Antibody (1066)
SKU: CBMAB-1066CQ
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

1066

Application

Immunogen

Recombinant protein of human CHP2.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

0.09% sodium azide

Concentration

Batch dependent

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Calcineurin Like EF-Hand Protein 2

Introduction

This gene product is a small calcium-binding protein that regulates cell pH by controlling plasma membrane-type Na+/H+ exchange activity. This protein shares sequence similarity with calcineurin B and can bind to and stimulate the protein phosphatase activity of calcineurin A (CnA) and functions in the calcineurin/NFAT (nuclear factor of activated T cells) signaling pathway. Functions as an integral cofactor in cell pH regulation by controlling plasma membrane-type Na(+)/H(+) exchange activity.

Entrez Gene ID

63928

UniProt ID

O43745

Alternative Names

HCA520

Function

Functions as an integral cofactor in cell pH regulation by controlling plasma membrane-type Na+/H+ exchange activity. Binds to and activates SLC9A1/NHE1 in a serum-independent manner, thus increasing pH and protecting cells from serum deprivation-induced death. Also plays a role in the regulation of cell proliferation and tumor growth by increasing the phosphatase activity of PPP3CA in a calcium-dependent manner. Activator of the calcineurin/NFAT signaling pathway. Involved in the cytoplasmic translocation of the transcription factor NFATC3 to the nucleus.

Biological Process

Cellular response to calcium ion Source: UniProtKB
Positive regulation of calcineurin-NFAT signaling cascade Source: UniProtKB
Positive regulation of cell population proliferation Source: UniProtKB
Positive regulation of phosphatase activity Source: UniProtKB
Positive regulation of protein import into nucleus Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: UniProtKB
Protein transport Source: UniProtKB-KW

Cellular Location

Cell membrane; Nucleus; Cytoplasm. Predominantly localized in a juxtanuclear region. Colocalizes with SLC9A3 in the juxtanuclear region and at the plasma membrane (By similarity). Exported from the nucleus to the cytoplasm through a nuclear export signal (NES) pathway. May shuttle between nucleus and cytoplasm.

Liang, S., Fuchs, S., Mymrikov, E. V., Stulz, A., Kaiser, M., Heerklotz, H., & Hunte, C. (2020). Calcium affects CHP1 and CHP2 conformation and their interaction with sodium/proton exchanger 1. The FASEB Journal, 34(2), 3253-3266.

Xu, L., Qin, Y., Sun, B., Wang, H., Gu, J., Tang, Z., ... & Feng, J. (2020). Involvement of CHP2 in the Development of Non–Small Cell Lung Cancer and Patients’ Poor Prognosis. Applied Immunohistochemistry & Molecular Morphology, 28(9), 678.

Zhao, X., Xie, T., Dai, T., Zhao, W., Li, J., Xu, R., ... & Ma, N. (2018). CHP2 promotes cell proliferation in breast cancer via suppression of FOXO3a. Molecular Cancer Research, 16(10), 1512-1522.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Related Products

Mouse Anti-CHP2 Recombinant Antibody (1065) (CAT#: CBMAB-1065CQ)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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