Mouse Anti-CHRNA3 Recombinant Antibody (CBT4015) (V2LY-0625-LY673)

Name: Mouse Anti-CHRNA3 Recombinant Antibody (CBT4015)
SKU: V2LY-0625-LY673
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-CHRNA3 Recombinant Antibody (CBT4015) (V2LY-0625-LY673) in ELISA

Mouse Anti-CHRNA3 Recombinant Antibody (CBT4015) (V2LY-0625-LY673) in WB

Mouse Anti-CHRNA3 Recombinant Antibody (CBT4015) (V2LY-0625-LY673) in IF

Mouse Anti-CHRNA3 Recombinant Antibody (CBT4015) (V2LY-0625-LY673) in FC

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBT4015

Antibody Isotype

IgG1

Application

ICC, FC

Basic Information

Immunogen

Purified recombinant fragment of human CHRNA3 (AA: 32-240) expressed in E. Coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
ICC	1:200-1:1,000
FC	1:200-1:400
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Entrez Gene ID

1136

UniProt ID

P32297

Function

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

Biological Process

Acetylcholine receptor signaling pathway Source: ARUK-UCL
Activation of transmembrane receptor protein tyrosine kinase activity Source: UniProtKB
Behavioral response to nicotine Source: UniProtKB
Chemical synaptic transmission Source: GO_Central
Excitatory postsynaptic potential Source: UniProtKB
Ion transmembrane transport Source: GO_Central
Ion transport Source: UniProtKB
Locomotory behavior Source: UniProtKB
Nervous system development Source: UniProtKB
Nervous system process Source: GO_Central
Regulation of acetylcholine secretion, neurotransmission Source: UniProtKB
Regulation of dendrite morphogenesis Source: UniProtKB
Regulation of membrane potential Source: UniProtKB
Regulation of smooth muscle contraction Source: UniProtKB
Response to acetylcholine Source: ARUK-UCL
Signal transduction Source: UniProtKB
Synaptic transmission, cholinergic Source: UniProtKB
Synaptic transmission involved in micturition Source: UniProtKB

Cellular Location

Postsynaptic cell membrane; Cell membrane

Involvement in disease

Bladder dysfunction, autonomic, with impaired pupillary reflex and secondary CAKUT (BAIPRCK): An autosomal recessive disease characterized by impaired innervation and autonomic dysfunction of the urinary bladder, hydronephrosis, vesicoureteral reflux, small kidneys, recurrent urinary tract infections, and progressive renal insufficiency. Additional autonomic features are impaired pupillary reflex and orthostatic hypotension. The disease manifests in utero or early childhood.

Topology

Extracellular: 32-240
Helical: 241-265
Helical: 273-291
Helical: 307-328
Cytoplasmic: 329-477
Helical: 478-497

More Infomation

References

Shibao, C., Joos, K., Phillips, J. A., Cogan, J., Newman, J. H., Hamid, R., ... & Biaggioni, I. (2021). Familial Autonomic Ganglionopathy Caused by Rare CHRNA3 Genetic Variants. Neurology.

Wu, T., Wang, Y., Shi, W., Zhang, B. Q., Raelson, J., Yao, Y. M., ... & Wu, J. (2020). A variant in the nicotinic acetylcholine receptor alpha 3 subunit gene is associated with hypertension risks in hypogonadic patients. Frontiers in genetics, 11, 1526.

Yang, X., Guo, X., Huang, Z., Da, Y., Xing, W., Li, F., ... & Zhu, Y. (2019). CHRNA5/CHRNA3 gene cluster is a risk factor for lumbar disc herniation: a case-control study. Journal of orthopaedic surgery and research, 14(1), 1-7.

Lee, S. H., Barrie, E. S., Sadee, W., & Smith, R. M. (2019). Nicotine Dependence and the CHRNA5/CHRNA3/CHRNB4 Nicotinic Receptor Regulome. In Neuroscience of Nicotine (pp. 347-353). Academic Press.

Shibao, C. A., Phillips, J., Cogan, J. D., Newman, J. H., Hamid, R., Meiler, J., ... & Biaggioni, I. (2019). Abstract P176: Familial Autonomic Ganglionopathy Caused by Rare CHRNA3 Genetic Variants. Novel Genetic Cause of Neurogenic Orthostatic Hypotension. Hypertension, 74(Suppl_1), AP176-AP176.

Shibao, C., Phillips, J. A., Cogan, J. D., Newman, J. H., Hamid, R., Sheehan, J., ... & Biaggioni, I. (2018). Familial Autonomic Ganglionopathy Caused by Rare CHRNA3 Genetic Variants: Novel Genetic Cause of Neurogenic Orthostatic Hypotension. Available at SSRN 3260799.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

Mouse Anti-CHRNA3 Recombinant Antibody (0072) (CAT#: CBMAB-0072-CN)

Mouse Anti-CHRNA3 Recombinant Antibody (0265) (CAT#: CBMAB-0265-CN)

Mouse Anti-CHRNA3 Recombinant Antibody (CBLNC-158) (CAT#: CBMAB-1371-CN)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

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SDS
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Second antibody and isotype control

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