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Mouse Anti-CHTF18 Recombinant Antibody (CBFYC-1866) (CBMAB-C1930-FY)


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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBFYC-1866
Antibody Isotype
IgG2a, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 887-975 mapping at the C-terminal of CTF18 of human.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
CTF18, chromosome transmission fidelity factor 18 homolog (S. cerevisiae)
Introduction
This gene encodes a protein which is a component of a replication factor C (RFC) complex, which loads proliferating cell nuclear antigen (PCNA) on to DNA during the S phase of cell cycle. The encoded protein may interact with other proteins, including RFC complex 3, to form a clamp loader complex that plays a role in sister chromatid cohesion during metaphase-anaphase transition.
Entrez Gene ID
63922
UniProt ID
Q8WVB6
Alternative Names
Chromosome Transmission Fidelity Factor 18; C16orf41; Ctf18; CHL12; CTF18, Chromosome Transmission Fidelity Factor 18 Homolog (S. Cerevisiae); Some Homology With Holliday Junction DNA Helicase RUVB Like; CTF18, Chromosome Transmission Fidelity Factor 18 Homolog; Chromosome Transmission Fidelity Protein 18 Homolog; Chromosome 16 Open Reading Frame 41; C321D2.3 (Novel Protein)
Function
Chromosome cohesion factor involved in sister chromatid cohesion and fidelity of chromosome transmission. Component of one of the cell nuclear antigen loader complexes, CTF18-replication factor C (CTF18-RFC), which consists of CTF18, CTF8, DCC1, RFC2, RFC3, RFC4 and RFC5. The CTF18-RFC complex binds to single-stranded and primed DNAs and has weak ATPase activity that is stimulated by the presence of primed DNA, replication protein A (RPA) and by proliferating cell nuclear antigen (PCNA). The CTF18-RFC complex catalyzes the ATP-dependent loading of PCNA onto primed and gapped DNA. Interacts with and stimulates DNA polymerase POLH. During DNA repair synthesis, involved in loading DNA polymerase POLE at the sites of local damage (PubMed:20227374).
Biological Process
DNA duplex unwinding Source: GOC
Positive regulation of DNA-directed DNA polymerase activity Source: UniProtKB
Telomere maintenance via semi-conservative replication Source: Reactome
Cellular Location
Nucleus. Associates with chromatin during S phase.
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For research use only. Not intended for any clinical use.

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