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Mouse Anti-CKAP2 Recombinant Antibody (3H1) (CBMAB-C6204-LY)

This product is antibody recognizes CKAP2. The antibody 3H1 immunoassay techniques such as: ELISA, IHC-P, WB.
See all CKAP2 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
3H1
Antibody Isotype
IgG1, κ
Application
ELISA, IHC-P, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
cytoskeleton associated protein 2
Introduction
This gene encodes a cytoskeleton-associated protein that stabalizes microtubules and plays a role in the regulation of cell division. The encoded protein is itself regulated through phosphorylation at multiple serine and threonine residues. There is a pseudogene of this gene on chromosome 14. Alternative splicing results in multiple transcript variations. [provided by RefSeq, Nov 2013]
Entrez Gene ID
26586
UniProt ID
Q8WWK9
Alternative Names
Cytoskeleton Associated Protein 2; Tumor- And Microtubule-Associated Protein; CTCL Tumor Antigen Se20-10; TMAP; LB1; Tumor-Associated Microtubule-Associated Protein; Cytoskeleton-Associated Protein 2; Se20-10;
Function
Possesses microtubule stabilizing properties. Involved in regulating aneuploidy, cell cycling, and cell death in a p53/TP53-dependent manner (By similarity).
Biological Process
Apoptotic process Source: UniProtKB-KW
Mitotic cytokinesis Source: MGI
Positive regulation of transcription by RNA polymerase II Source: MGI
Cellular Location
Cytoskeleton; Spindle; Spindle pole. Contrary to the ectopically expressed protein, endogenous CKAP2 does not colocalize with microtubules in G1, S and early G2. At late G2 and prophase after separation of duplicated centrosomes, colocalizes with gamma-tubulin and centrosome-proximal microtubules. From prometaphase through anaphase B, colocalizes with mitotic spindle poles and spindle microtubules. During cytokinesis, absent from midbody microtubules.

McAlear, T. S., & Bechstedt, S. (2022). The mitotic spindle protein CKAP2 potently increases formation and stability of microtubules. Elife, 11, e72202.

Feng, Y., Wei, G., Zhang, L., Zhou, H., Wang, W., Guo, P., ... & Tu, H. (2021). LncRNA DARS-AS1 aggravates the growth and metastasis of hepatocellular carcinoma via regulating the miR-3200-5p-Cytoskeleton associated protein 2 (CKAP2) axis. Bioengineered, 12(1), 8217-8232.

Chen, X., Xie, J., Cui, Y., Zhang, L., Yu, H., Chen, J., ... & Meng, Q. (2021). Cytoskeleton-associated protein 2 (CKAP2) is regulated by vascular endothelial growth factor and p53 in retinal capillary endothelial cells under high-glucose conditions. Molecular and Cellular Endocrinology, 535, 111378.

Mamoor, S. (2021). Over-expression of cytoskeleton associated protein 2 in human endometrial cancer.

Zhang, C., Zhang, X., Han, Z., Zhu, H., & Wan, T. (2020). Silence of cytoskeleton-associated protein 2 represses cell proliferation and migration and promotes apoptosis in liver cancer cell lines. Zhong nan da xue xue bao. Yi xue ban= Journal of Central South University. Medical Sciences, 45(4), 365-371.

Wang, K., Huang, R., Li, G., Zeng, F., Zhao, Z., Liu, Y., ... & Jiang, T. (2018). CKAP2 expression is associated with glioma tumor growth and acts as a prognostic factor in high‑grade glioma. Oncology reports, 40(4), 2036-2046.

Zhang, S., Wang, Y., Chen, S., & Li, J. (2018). Silencing of cytoskeleton-associated protein 2 represses cell proliferation and induces cell cycle arrest and cell apoptosis in osteosarcoma cells. Biomedicine & Pharmacotherapy, 106, 1396-1403.

Sim, S. H., Bae, C. D., Kwon, Y., Hwang, H. L., Poojan, S., Hong, H. I., ... & Ro, J. (2017). CKAP2 (cytoskeleton-associated protein2) is a new prognostic marker in HER2-negative luminal type breast cancer. PLoS One, 12(8), e0182107.

Guo, Q. S., Song, Y., Hua, K. Q., & Gao, S. J. (2017). Involvement of FAK-ERK2 signaling pathway in CKAP2-induced proliferation and motility in cervical carcinoma cell lines. Scientific reports, 7(1), 1-12.

Zhang, M., & Zhao, L. (2017). CKAP2 promotes ovarian cancer proliferation and tumorigenesis through the FAK-ERK pathway. DNA and cell biology, 36(11), 983-990.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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