Mouse Anti-CKM Recombinant Antibody (CBYY-C0252) (CBMAB-C0736-YY)

Name: Mouse Anti-CKM Recombinant Antibody (CBYY-C0252)
SKU: CBMAB-C0736-YY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CKM Recombinant Antibody (CBYY-C0252) (CBMAB-C0736-YY) in WB

Western blotting of individual proteins in ALS compared to controls normalized to the housekeeper protein transferrin (Transf), and optical density (OD) measurements of the bands. ...View More

Katzeff, J. S., Bright, F., Lo, K., Kril, J. J., Connolly, A., Crossett, B., ... & Kim, W. S. (2020). Altered serum protein levels in frontotemporal dementia and amyotrophic lateral sclerosis indicate calcium and immunity dysregulation. Scientific reports, 10(1), 13741.

Distributed under Open Access license CC BY 4.0, without modification.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYY-C0252

Application

WB, IP, IF, ELISA, IHC-P

Immunogen

Amino acids 9-27 near the N-terminus of creatine kinase-M of human.

Host Species

Mouse

Specificity

Human, Mouse, Rat, Horse

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Creatine Kinase, M-Type

Entrez Gene ID

1158

UniProt ID

P06732

Alternative Names

C-Type Lectin Domain Containing 9A; C-Type Lectin Domain Family 9 Member A; C-Type Lectin Domain Family 9, Member A; HEEE9341; UNQ9341; DNGR-1; CD370; DNGR1;

Function

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

Biological Process

Creatine metabolic process Source: Reactome
Phosphocreatine biosynthetic process Source: GO_Central

Cellular Location

Cytoplasm

Fernández-Torres, J., Martínez-Nava, G. A., Zamudio-Cuevas, Y., Barbier, O. C., Narváez-Morales, J., & Martínez-Flores, K. (2021). Ancestral contribution of the muscle-specific creatine kinase (CKM) polymorphism rs4884 in the knee osteoarthritis risk: a preliminary study. Clinical Rheumatology, 40(1), 279-285.

Dybus, A., Yu, Y. H., Proskura, W., Lanckriet, R., & Cheng, Y. H. (2020). Association of Sequence Variants in the CKM (Creatine Kinase, M-Type) Gene with Racing Performance of Homing Pigeons. Russian Journal of Genetics, 56(8), 1006-1011.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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