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Mouse Anti-CLC Recombinant Antibody (CBYCL-112) (CBMAB-L0013-YC)

Provided herein is a Mouse monoclonal antibody against Human CLC. The antibody can be used for immunoassay techniques, such as WB, ICC, IHC-P, IHC-Fr, ELISA.
See all CLC antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYCL-112
Antibody Isotype
IgG
Application
WB, ICC, IHC-P, IHC-Fr, ELISA

Basic Information

Immunogen
Charcot Leyden Crystal Protein
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH7.4, with 0.02% sodium azide, 50% glycerol
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Charcot-Leyden Crystal Galectin
Introduction
Lysophospholipases are enzymes that act on biological membranes to regulate the multifunctional lysophospholipids. The protein encoded by this gene is a lysophospholipase expressed in eosinophils and basophils. It hydrolyzes lysophosphatidylcholine to glycerophosphocholine and a free fatty acid. This protein may possess carbohydrate or IgE-binding activities. It is both structurally and functionally related to the galectin family of beta-galactoside binding proteins. It may be associated with inflammation and some myeloid leukemias.
Entrez Gene ID
1178
UniProt ID
Q05315
Alternative Names
GAL10; Gal-10; LGALS10; LGALS10A; LPPL_HUMAN
Function
Regulates immune responses through the recognition of cell-surface glycans. Essential for the anergy and suppressive function of CD25-positive regulatory T-cells (Treg).
Biological Process
Multicellular organism development Source: ProtInc
Regulation of activated T cell proliferation Source: UniProtKB
Regulation of T cell anergy Source: UniProtKB
Regulation of T cell cytokine production Source: UniProtKB
T cell apoptotic process Source: UniProtKB
Cellular Location
Cytosol; Cytoplasmic granule. Localized in granules from where it may be secreted or transported to other locations in the cell.

Zhang, M., Yan, B., Wang, Y., Wang, C., & Zhang, L. (2020). Charcot-Leyden Crystal Protein in Nasal Secretions of Patients with Nonallergic Rhinitis with Eosinophilia Syndrome. International Archives of Allergy and Immunology, 181(11), 888-896.

Grozdanovic, M. M., Doyle, C. B., Liu, L., Maybruck, B. T., Kwatia, M. A., Thiyagarajan, N., ... & Ackerman, S. J. (2020). Charcot-Leyden crystal protein/galectin-10 interacts with cationic ribonucleases and is required for eosinophil granulogenesis. Journal of Allergy and Clinical Immunology, 146(2), 377-389.

Weller, P. F., Wang, H., & Melo, R. C. (2020). The Charcot–Leyden crystal protein revisited—A lysopalmitoylphospholipase and more. Journal of leukocyte biology, 108(1), 105-112.

Melo, R. C., Wang, H., Silva, T. P., Imoto, Y., Fujieda, S., Fukuchi, M., ... & Weller, P. F. (2020). Galectin‐10, the protein that forms Charcot‐Leyden crystals, is not stored in granules but resides in the peripheral cytoplasm of human eosinophils. Journal of leukocyte biology, 108(1), 139-149.

Nyenhuis, S. M., Alumkal, P., Du, J., Maybruck, B. T., Vinicky, M., & Ackerman, S. J. (2019). Charcot–Leyden crystal protein/galectin-10 is a surrogate biomarker of eosinophilic airway inflammation in asthma. Biomarkers in medicine, 13(9), 715-724.

Ueki, S., Miyabe, Y., Yamamoto, Y., Fukuchi, M., Hirokawa, M., Spencer, L. A., & Weller, P. F. (2019). Charcot-Leyden crystals in eosinophilic inflammation: active cytolysis leads to crystal formation. Current allergy and asthma reports, 19(8), 1-9.

Rodríguez-Alcázar, J. F., Ataide, M. A., Engels, G., Schmitt-Mabmunyo, C., Garbi, N., Kastenmüller, W., ... & Franklin, B. S. (2019). Charcot–Leyden crystals activate the NLRP3 inflammasome and cause IL-1β inflammation in human macrophages. The Journal of Immunology, 202(2), 550-558.

Su, J. (2018). A brief history of Charcot-Leyden crystal protein/galectin-10 research. Molecules, 23(11), 2931.

Ueki, S., Tokunaga, T., Melo, R. C., Saito, H., Honda, K., Fukuchi, M., ... & Weller, P. F. (2018). Charcot-Leyden crystal formation is closely associated with eosinophil extracellular trap cell death. Blood, The Journal of the American Society of Hematology, 132(20), 2183-2187.

Klion, A. D. (2018). Charcot-Leyden crystals: solving an enigma. Blood, The Journal of the American Society of Hematology, 132(20), 2111-2112.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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