Mouse Anti-CLCA2 Recombinant Antibody (1D5) (CBMAB-C3845-LY)

Name: Mouse Anti-CLCA2 Recombinant Antibody (1D5)
SKU: CBMAB-C3845-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

1D5

Application

WB, IP, ELISA

Immunogen

Amino acids 300-400 representing partial length CLCA2 of human.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.1 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

chloride channel regulator 2

Introduction

This gene encodes a member of the calcium-activated chloride channel regulator (CLCR) family of proteins. Members of this family regulate the transport of chloride across the plasma membrane. The encoded protein is autoproteolytically processed to generate N- and C- terminal fragments. Expression of this gene is upregulated by the tumor suppressor protein p53 in response to DNA damage. In breast cancer, expression of this gene is downregulated and the encoded protein may inhibit migration and invasion while promoting mesenchymal-to-epithelial transition in cancer cell lines. [provided by RefSeq, Sep 2016]

Entrez Gene ID

9635

UniProt ID

Q9UQC9

Alternative Names

Chloride Channel Accessory 2; Chloride Channel, Calcium Activated, Family Member 2; Calcium-Activated Chloride Channel Protein 3; CaCC-3; CACC3; Calcium-Activated Chloride Channel Family Member 2; CLCA Family Member 2, Chloride Channel Regulator; Calcium-Activated Chloride Channel Regulator 2;

Function

Plays a role in modulating chloride current across the plasma membrane in a calcium-dependent manner, and cell adhesion. Involved in basal cell adhesion and/or stratification of squamous epithelia. May act as a tumor suppressor in breast and colorectal cancer. Plays a key role for cell adhesion in the beginning stages of lung metastasis via the binding to ITGB4.

Biological Process

Cell adhesion Source: UniProtKB-KW
Chloride transport Source: Ensembl
Ion transmembrane transport Source: Reactome

Cellular Location

Cell membrane; Basal cell membrane; Cell junction
Calcium-activated chloride channel regulator 2, 109 kDa form: Secreted. Remains associated to the 35 kDa form until an unidentified event triggers the release.

Topology

Extracellular: 32-901
Helical: 902-922
Cytoplasmic: 923-943

PTM

The 141 kDa mature form is autoproteolytically cleaved by the metalloprotease domain, producing a 109 kDa form and a 35 kDa form. The cleavage is necessary for calcium-activated chloride channel (CaCC) activation activity.
N-glycosylated.

Seltmann, K., Meyer, M., Sulcova, J., Kockmann, T., Wehkamp, U., Weidinger, S., ... & Werner, S. (2018). Humidity-regulated CLCA2 protects the epidermis from hyperosmotic stress. Science translational medicine, 10(440).

Sharma, A., Ramena, G., Yin, Y., Premkumar, L., & Elble, R. C. (2018). CLCA2 is a positive regulator of store-operated calcium entry and TMEM16A. PLoS One, 13(5), e0196512.

Porretti, J., Dalton, G. N., Massillo, C., Scalise, G. D., Farré, P. L., Elble, R., ... & De Siervi, A. (2018). CLCA2 epigenetic regulation by CTBP1, HDACs, ZEB1, EP300 and miR‐196b‐5p impacts prostate cancer cell adhesion and EMT in metabolic syndrome disease. International journal of cancer, 143(4), 897-906.

Yin, Y., & Elble, R. C. (2018). The role of CLCA2 in cell proliferation and survival of HNSCC—potential biomarker for sensitivity to EGFR inhibitors.

Qiang, Y. Y., Li, C. Z., Sun, R., Zheng, L. S., Peng, L. X., Yang, J. P., ... & Qian, C. N. (2018). Along with its favorable prognostic role, CLCA2 inhibits growth and metastasis of nasopharyngeal carcinoma cells via inhibition of FAK/ERK signaling. Journal of Experimental & Clinical Cancer Research, 37(1), 1-14.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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