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Mouse Anti-CLSPN Recombinant Antibody (CBXC-1212) (CBMAB-C5752-CQ)


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Published Data
CBMAB-C5752-CQ-1
Mouse Anti-CLSPN Recombinant Antibody (CBXC-1212) (CBMAB-C5752-CQ) in WB
Western blot of CDC45-GFP, MCM4-Halo cell line treated with control siRNA and siRNA against CLASPIN (abbreviated as CLSN), TIMELESS (abbreviated as TIM) and AND1 (5 nM; 24 h) and ATR inhibitor; n = 2 biological replicates. ...View More
Polasek-Sedlackova, H., Miller, T. C., Krejci, J., Rask, M. B., & Lukas, J. (2022). Solving the MCM paradox by visualizing the scaffold of CMG helicase at active replisomes. Nature Communications, 13(1), 6090.
Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-C5752-CQ-2
Mouse Anti-CLSPN Recombinant Antibody (CBXC-1212) (CBMAB-C5752-CQ) in IF
Representative MIP confocal images of chromatin-bound CDC45-GFP, MCM4-Halo and immunostained MCM2 in S5 stage of S phase sub-stratified. Cells were transfected by control siRNA or siRNA against CLASPIN as indicated and treated with ATR inhibitor (ATRi). Scale bar, 10 μm. c, Colocalization of CDC45-GFP and immunostained MCM2 in the S5 stage of S phase. ...View More
Polasek-Sedlackova, H., Miller, T. C., Krejci, J., Rask, M. B., & Lukas, J. (2022). Solving the MCM paradox by visualizing the scaffold of CMG helicase at active replisomes. Nature Communications, 13(1), 6090.
Distributed under Open Access license CC BY 4.0, without modification.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBXC-1212
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 1033-1332 mapping at the C-terminus of Claspin of human.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
0.1% gelatin
Preservative
<0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Claspin
Introduction
The product of this gene is an essential upstream regulator of checkpoint kinase 1 and triggers a checkpoint arrest of the cell cycle in response to replicative stress or DNA damage. The protein is also required for efficient DNA replication during a normal S phase. Multiple transcript variants encoding different isoforms have been found for this gene.
Entrez Gene ID
63967
UniProt ID
Q9HAW4
Alternative Names
Claspin; Claspin Homolog (Xenopus Laevis); Claspin Homolog; HClaspin;
Function
Required for checkpoint mediated cell cycle arrest in response to inhibition of DNA replication or to DNA damage induced by both ionizing and UV irradiation. Adapter protein which binds to BRCA1 and the checkpoint kinase CHEK1 and facilitates the ATR-dependent phosphorylation of both proteins. Can also bind specifically to branched DNA structures and may associate with S-phase chromatin following formation of the pre-replication complex (pre-RC). This may indicate a role for this protein as a sensor which monitors the integrity of DNA replication forks.
Biological Process
Activation of protein kinase activity Source: UniProtKB
DNA damage checkpoint Source: UniProtKB
DNA repair Source: UniProtKB-KW
DNA replication Source: Reactome
DNA replication checkpoint Source: MGI
Mitotic DNA replication checkpoint Source: UniProtKB
Mitotic G2 DNA damage checkpoint Source: UniProtKB
Peptidyl-serine phosphorylation Source: UniProtKB
Protein deubiquitination Source: Reactome
Cellular Location
Nucleus
PTM
Phosphorylated. Undergoes ATR-dependent phosphorylation by CHEK1 during activation of DNA replication or damage checkpoints. Phosphorylation by CSNK1G1/CK1 promotes CHEK1 binding.
Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) during G1 phase, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Following DNA damage, it is deubiquitinated by USP28 in G2 phase, preventing its degradation.
More Infomation

Cai, C., Luo, J., Liu, Q., Liu, Z., Zhao, Y., Wu, X., ... & Liu, Y. (2021). Claspin overexpression promotes tumor progression and predicts poor clinical outcome in prostate cancer. Genetic Testing and Molecular Biomarkers, 25(2), 131-139.

Babasaki, T., Sentani, K., Sekino, Y., Kobayashi, G., Thang Pham, Q., Katsuya, N., ... & Yasui, W. (2021). Overexpression of claspin promotes docetaxel resistance and is associated with prostate‐specific antigen recurrence in prostate cancer. Cancer Medicine, 10(16), 5574-5588.

Mamoor, S. (2021). Differential expression of claspin in triple negative breast cancer.

Kobayashi, G., Sentani, K., Babasaki, T., Sekino, Y., Shigematsu, Y., Hayashi, T., ... & Yasui, W. (2020). Claspin overexpression is associated with high‐grade histology and poor prognosis in renal cell carcinoma. Cancer science, 111(3), 1020.

Azenha, D., Hernandez-Perez, S., Martin, Y., Viegas, M. S., Martins, A., Lopes, M. C., ... & Martins, T. C. (2020). Implications of CLSPN Variants in Cellular Function and Susceptibility to Cancer. Cancers, 12(9), 2396.

Kobayashi, G., Sentani, K., Hattori, T., Yamamoto, Y., Imai, T., Sakamoto, N., ... & Yasui, W. (2019). Clinicopathological significance of claspin overexpression and its association with spheroid formation in gastric cancer. Human pathology, 84, 8-17.

Bianco, J. N., Bergoglio, V., Lin, Y. L., Pillaire, M. J., Schmitz, A. L., Gilhodes, J., ... & Pasero, P. (2019). Overexpression of Claspin and Timeless protects cancer cells from replication stress in a checkpoint-independent manner. Nature communications, 10(1), 1-14.

Smits, V. A., Cabrera, E., Freire, R., & Gillespie, D. A. (2019). Claspin–checkpoint adaptor and DNA replication factor. The FEBS journal, 286(3), 441-455.

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For research use only. Not intended for any clinical use.

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